ENSG00000140474

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000440863 ENSG00000140474 HEK293_OSMI2_2hA HEK293_TMG_2hB ULK3 protein_coding protein_coding 60.03109 87.41029 43.05647 6.003267 1.427992 -1.021403 17.370899 31.297492 11.000898 2.7988992 1.9538690 -1.5075758 0.27365833 0.35716667 0.25610000 -0.10106667 2.866753e-01 8.427338e-06 FALSE TRUE
ENST00000567472 ENSG00000140474 HEK293_OSMI2_2hA HEK293_TMG_2hB ULK3 protein_coding retained_intron 60.03109 87.41029 43.05647 6.003267 1.427992 -1.021403 3.178530 4.714136 1.509996 0.9878233 0.4404120 -1.6359832 0.05030833 0.05610000 0.03556667 -0.02053333 6.078204e-01 8.427338e-06 FALSE TRUE
ENST00000568718 ENSG00000140474 HEK293_OSMI2_2hA HEK293_TMG_2hB ULK3 protein_coding retained_intron 60.03109 87.41029 43.05647 6.003267 1.427992 -1.021403 3.271396 2.942820 2.065571 0.3183919 0.4045428 -0.5085853 0.06008333 0.03346667 0.04746667 0.01400000 3.274123e-01 8.427338e-06 FALSE FALSE
ENST00000569437 ENSG00000140474 HEK293_OSMI2_2hA HEK293_TMG_2hB ULK3 protein_coding protein_coding 60.03109 87.41029 43.05647 6.003267 1.427992 -1.021403 17.486400 32.397018 11.415839 1.2879875 0.9531945 -1.5040062 0.27322917 0.37220000 0.26443333 -0.10776667 2.023116e-03 8.427338e-06 FALSE TRUE
ENST00000570276 ENSG00000140474 HEK293_OSMI2_2hA HEK293_TMG_2hB ULK3 protein_coding retained_intron 60.03109 87.41029 43.05647 6.003267 1.427992 -1.021403 7.713784 5.283827 8.091964 0.5651622 0.8812070 0.6139606 0.14726667 0.06190000 0.18700000 0.12510000 8.427338e-06 8.427338e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000140474 E001 4.014725 0.0041054058 1.722442e-01 2.911285e-01 15 74836118 74836131 14 - 0.475 0.704 1.031
ENSG00000140474 E002 1295.962019 0.0020279114 1.065703e-04 5.522424e-04 15 74836132 74836632 501 - 2.982 3.080 0.328
ENSG00000140474 E003 776.751408 0.0021318770 2.357772e-04 1.115706e-03 15 74836633 74836804 172 - 2.755 2.859 0.346
ENSG00000140474 E004 417.877347 0.0033670095 2.403972e-03 8.473467e-03 15 74836805 74836892 88 - 2.482 2.592 0.368
ENSG00000140474 E005 397.029646 0.0037439761 2.700238e-02 6.600160e-02 15 74836893 74836963 71 - 2.479 2.565 0.287
ENSG00000140474 E006 282.595556 0.0007525992 2.334090e-04 1.105832e-03 15 74836964 74836972 9 - 2.315 2.421 0.353
ENSG00000140474 E007 755.059734 0.0004063856 1.743536e-03 6.419350e-03 15 74836973 74837198 226 - 2.776 2.835 0.195
ENSG00000140474 E008 308.971166 0.0004689612 7.671218e-02 1.542315e-01 15 74837199 74837212 14 - 2.397 2.445 0.160
ENSG00000140474 E009 366.376422 0.0009686142 7.365843e-02 1.493868e-01 15 74837213 74837244 32 - 2.469 2.519 0.169
ENSG00000140474 E010 436.509152 0.0001653991 3.127511e-02 7.443531e-02 15 74837369 74837435 67 - 2.546 2.593 0.155
ENSG00000140474 E011 127.346541 0.0002474644 2.917252e-01 4.326244e-01 15 74837751 74837756 6 - 2.074 2.041 -0.111
ENSG00000140474 E012 322.132196 0.0001763413 4.619797e-01 6.019013e-01 15 74837757 74837798 42 - 2.434 2.454 0.069
ENSG00000140474 E013 36.330663 0.0098918957 3.604145e-08 3.994585e-07 15 74837799 74837956 158 - 1.772 1.365 -1.392
ENSG00000140474 E014 285.964507 0.0001642616 3.028042e-01 4.447236e-01 15 74838152 74838173 22 - 2.376 2.404 0.095
ENSG00000140474 E015 276.201718 0.0001792895 2.494329e-01 3.852495e-01 15 74838174 74838192 19 - 2.358 2.390 0.107
ENSG00000140474 E016 415.062239 0.0003749566 1.529788e-01 2.657184e-01 15 74838266 74838344 79 - 2.531 2.566 0.118
ENSG00000140474 E017 403.841976 0.0001483777 8.502581e-01 9.071288e-01 15 74838440 74838504 65 - 2.540 2.548 0.026
ENSG00000140474 E018 8.686178 0.0023351603 9.901246e-08 1.006148e-06 15 74838505 74838512 8 - 1.276 0.704 -2.139
ENSG00000140474 E019 450.563321 0.0001591062 1.336299e-01 2.391930e-01 15 74838643 74838745 103 - 2.570 2.603 0.110
ENSG00000140474 E020 363.461758 0.0001320705 2.309403e-01 3.636005e-01 15 74839010 74839050 41 - 2.479 2.508 0.098
ENSG00000140474 E021 478.705711 0.0001753531 9.086790e-01 9.459847e-01 15 74839268 74839373 106 - 2.617 2.619 0.007
ENSG00000140474 E022 11.221040 0.0017790887 3.749864e-07 3.399696e-06 15 74839523 74839557 35 - 1.329 0.831 -1.818
ENSG00000140474 E023 484.100130 0.0001831534 6.281207e-01 7.437596e-01 15 74839558 74839713 156 - 2.615 2.628 0.044
ENSG00000140474 E024 180.300907 0.0002164579 1.995481e-01 3.256646e-01 15 74840234 74840241 8 - 2.223 2.189 -0.114
ENSG00000140474 E025 194.618302 0.0002075609 9.635640e-02 1.848999e-01 15 74840242 74840264 23 - 2.263 2.219 -0.145
ENSG00000140474 E026 238.419051 0.0001941800 5.135514e-05 2.885425e-04 15 74840265 74840296 32 - 2.388 2.290 -0.328
ENSG00000140474 E027 225.962446 0.0001886165 7.762034e-02 1.556441e-01 15 74840297 74840316 20 - 2.325 2.282 -0.142
ENSG00000140474 E028 44.269877 0.0190208894 6.724988e-08 7.061853e-07 15 74840317 74840318 2 - 1.872 1.428 -1.510
ENSG00000140474 E029 101.564277 0.0138163747 8.876031e-08 9.098736e-07 15 74840319 74840497 179 - 2.177 1.831 -1.163
ENSG00000140474 E030 326.476515 0.0001734862 6.944541e-01 7.952841e-01 15 74840498 74840586 89 - 2.457 2.452 -0.016
ENSG00000140474 E031 186.560059 0.0002111170 8.625507e-01 9.153618e-01 15 74840587 74840594 8 - 2.213 2.212 -0.004
ENSG00000140474 E032 231.081514 0.0002048268 5.675212e-01 6.942959e-01 15 74840595 74840614 20 - 2.313 2.301 -0.037
ENSG00000140474 E033 240.609188 0.0008325095 2.205133e-01 3.510259e-01 15 74840615 74840641 27 - 2.343 2.313 -0.099
ENSG00000140474 E034 274.426156 0.0034167184 6.142715e-01 7.327940e-01 15 74841405 74841448 44 - 2.386 2.378 -0.026
ENSG00000140474 E035 290.975712 0.0045585443 4.219231e-01 5.650747e-01 15 74841449 74841493 45 - 2.422 2.401 -0.071
ENSG00000140474 E036 212.689006 0.0025561518 4.925347e-01 6.296934e-01 15 74841494 74841505 12 - 2.282 2.265 -0.057
ENSG00000140474 E037 202.577395 0.0015621609 1.251453e-01 2.273467e-01 15 74841506 74841509 4 - 2.281 2.237 -0.149
ENSG00000140474 E038 280.192339 0.0011435283 7.603467e-02 1.531681e-01 15 74842075 74842118 44 - 2.422 2.378 -0.147
ENSG00000140474 E039 319.155596 0.0007003813 8.038741e-04 3.272905e-03 15 74842119 74842195 77 - 2.501 2.424 -0.258
ENSG00000140474 E040 52.297824 0.0051760169 1.234648e-03 4.758424e-03 15 74842196 74842279 84 - 1.796 1.605 -0.649
ENSG00000140474 E041 374.106140 0.0027212438 1.034161e-05 6.851891e-05 15 74842280 74842417 138 - 2.606 2.477 -0.432
ENSG00000140474 E042 130.194238 0.0009926959 1.501800e-09 2.154574e-08 15 74842418 74842420 3 - 2.199 1.990 -0.702
ENSG00000140474 E043 11.262792 0.0094182995 5.206244e-04 2.236981e-03 15 74842421 74842449 29 - 1.264 0.896 -1.339
ENSG00000140474 E044 9.083984 0.0020520228 2.425068e-03 8.537061e-03 15 74842450 74842534 85 - 1.158 0.830 -1.216
ENSG00000140474 E045 6.489370 0.0025630882 3.739593e-04 1.673449e-03 15 74842535 74842538 4 - 1.094 0.666 -1.653
ENSG00000140474 E046 9.542343 0.0018991904 2.844057e-03 9.806671e-03 15 74842539 74842545 7 - 1.173 0.857 -1.165
ENSG00000140474 E047 21.766218 0.0083499683 5.103337e-08 5.491377e-07 15 74842546 74842650 105 - 1.582 1.128 -1.583
ENSG00000140474 E048 16.633903 0.0129444505 1.008248e-06 8.356354e-06 15 74842651 74842674 24 - 1.481 1.009 -1.670
ENSG00000140474 E049 13.767203 0.0287636847 4.469999e-07 3.992776e-06 15 74842675 74842698 24 - 1.460 0.861 -2.154
ENSG00000140474 E050 197.213070 0.0076430465 3.874801e-04 1.726532e-03 15 74843004 74843346 343 - 2.352 2.188 -0.546