ENSG00000140396

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000452400 ENSG00000140396 HEK293_OSMI2_2hA HEK293_TMG_2hB NCOA2 protein_coding protein_coding 4.075828 1.562573 6.643595 0.05120447 0.2180253 2.081007 3.1357320 1.519582376 4.7327774 0.009342641 0.1015678 1.632594 0.83430417 0.974166667 0.71483333 -0.25933333 1.186999e-04 3.926516e-05 FALSE TRUE
ENST00000518363 ENSG00000140396 HEK293_OSMI2_2hA HEK293_TMG_2hB NCOA2 protein_coding protein_coding 4.075828 1.562573 6.643595 0.05120447 0.2180253 2.081007 0.3381404 0.000000000 0.8332592 0.000000000 0.1475052 6.397904 0.04977917 0.000000000 0.12433333 0.12433333 3.926516e-05 3.926516e-05 FALSE TRUE
MSTRG.31613.10 ENSG00000140396 HEK293_OSMI2_2hA HEK293_TMG_2hB NCOA2 protein_coding   4.075828 1.562573 6.643595 0.05120447 0.2180253 2.081007 0.1681956 0.007604763 0.3910727 0.007604763 0.1616587 4.509826 0.02760417 0.004566667 0.05743333 0.05286667 1.657187e-01 3.926516e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000140396 E001 271.1633093 0.0070438004 1.084710e-41 4.193777e-39 8 70109782 70112069 2288 - 2.141 2.730 1.965
ENSG00000140396 E002 93.6208667 0.0069528396 1.346187e-02 3.699265e-02 8 70112070 70113332 1263 - 1.812 2.070 0.870
ENSG00000140396 E003 29.8090211 0.0035557422 7.928218e-01 8.675230e-01 8 70113333 70113568 236 - 1.371 1.475 0.360
ENSG00000140396 E004 20.2185074 0.0009583730 6.315530e-01 7.465359e-01 8 70113569 70113643 75 - 1.215 1.296 0.282
ENSG00000140396 E005 19.6152435 0.0044882632 5.873266e-01 7.106278e-01 8 70121302 70121391 90 - 1.208 1.279 0.252
ENSG00000140396 E006 0.4355181 0.6346947599 9.358317e-01 9.636296e-01 8 70123804 70123883 80 - 0.158 0.000 -9.473
ENSG00000140396 E007 31.6421073 0.0008365552 1.882182e-01 3.114969e-01 8 70123884 70124082 199 - 1.414 1.442 0.096
ENSG00000140396 E008 35.1308363 0.0056193361 8.170362e-03 2.421506e-02 8 70124688 70124865 178 - 1.482 1.393 -0.309
ENSG00000140396 E009 1.1940256 0.0188177897 5.152288e-01 6.499201e-01 8 70126609 70126812 204 - 0.307 0.236 -0.514
ENSG00000140396 E010 42.9491932 0.0005710022 4.187225e-04 1.846045e-03 8 70126813 70127047 235 - 1.568 1.453 -0.393
ENSG00000140396 E011 19.4779628 0.0009803944 3.081510e-02 7.355485e-02 8 70128433 70128510 78 - 1.234 1.146 -0.316
ENSG00000140396 E012 32.9559282 0.0006093352 2.099405e-03 7.532261e-03 8 70128702 70128980 279 - 1.454 1.341 -0.393
ENSG00000140396 E013 25.5882286 0.0078242438 4.728745e-02 1.043468e-01 8 70131837 70132002 166 - 1.343 1.279 -0.223
ENSG00000140396 E014 0.0000000       8 70137973 70138202 230 -      
ENSG00000140396 E015 20.8331965 0.0026154624 1.265693e-03 4.862643e-03 8 70138203 70138332 130 - 1.280 1.072 -0.741
ENSG00000140396 E016 0.0000000       8 70141179 70141183 5 -      
ENSG00000140396 E017 27.6595589 0.0131481831 4.688112e-04 2.040924e-03 8 70141184 70141399 216 - 1.400 1.147 -0.889
ENSG00000140396 E018 30.3986586 0.0006258730 1.576877e-03 5.883847e-03 8 70144642 70144848 207 - 1.427 1.295 -0.455
ENSG00000140396 E019 26.0737770 0.0007993936 3.565192e-02 8.290759e-02 8 70148273 70148483 211 - 1.348 1.296 -0.184
ENSG00000140396 E020 50.7203354 0.0320396238 1.684400e-02 4.458698e-02 8 70155971 70156640 670 - 1.638 1.518 -0.407
ENSG00000140396 E021 8.6777286 0.0019702924 1.317052e-01 2.365084e-01 8 70156641 70156665 25 - 0.921 0.830 -0.349
ENSG00000140396 E022 32.7094553 0.0005998409 2.336262e-05 1.426125e-04 8 70156666 70157124 459 - 1.468 1.245 -0.773
ENSG00000140396 E023 16.4456112 0.0017041619 6.308193e-04 2.646634e-03 8 70157125 70157240 116 - 1.191 0.914 -1.011
ENSG00000140396 E024 18.6060744 0.0009804706 5.312287e-04 2.276452e-03 8 70159505 70159652 148 - 1.238 0.985 -0.914
ENSG00000140396 E025 22.7456893 0.0019097886 5.378180e-07 4.727763e-06 8 70162711 70162854 144 - 1.340 0.914 -1.533
ENSG00000140396 E026 22.9537210 0.0008032887 1.482293e-08 1.770257e-07 8 70163465 70163566 102 - 1.348 0.831 -1.884
ENSG00000140396 E027 27.2543517 0.0067108993 1.177416e-09 1.721657e-08 8 70166566 70166754 189 - 1.424 0.831 -2.145
ENSG00000140396 E028 23.0562980 0.0012195901 7.010936e-08 7.333264e-07 8 70170202 70170379 178 - 1.348 0.874 -1.714
ENSG00000140396 E029 16.9420779 0.0010909369 6.634865e-06 4.593967e-05 8 70174756 70174859 104 - 1.219 0.782 -1.625
ENSG00000140396 E030 23.9816496 0.0009845486 2.306607e-02 5.788902e-02 8 70213903 70214075 173 - 1.322 1.245 -0.269
ENSG00000140396 E031 13.8138292 0.0012957031 1.703999e-02 4.502400e-02 8 70216660 70216695 36 - 1.110 0.951 -0.585
ENSG00000140396 E032 11.9177864 0.0014648635 8.403013e-02 1.658480e-01 8 70216696 70216720 25 - 1.041 0.951 -0.333
ENSG00000140396 E033 12.2091639 0.0014641038 3.255387e-02 7.690968e-02 8 70216721 70216764 44 - 1.058 0.914 -0.532
ENSG00000140396 E034 1.0675023 0.0116489732 6.322875e-01 7.471601e-01 8 70245007 70245375 369 - 0.274 0.236 -0.289
ENSG00000140396 E035 11.9399997 0.0016217085 7.984588e-04 3.254004e-03 8 70296744 70296800 57 - 1.069 0.727 -1.309
ENSG00000140396 E036 0.2924217 0.0271295728 1.000000e+00   8 70401814 70402079 266 - 0.112 0.000 -9.578
ENSG00000140396 E037 0.9244060 0.0136968632 7.759401e-01 8.557111e-01 8 70402163 70402587 425 - 0.239 0.236 -0.026
ENSG00000140396 E038 2.9172196 0.0663172194 2.779214e-01 4.175599e-01 8 70403198 70403421 224 - 0.544 0.389 -0.784
ENSG00000140396 E039 7.0632628 0.0023916008 5.018495e-02 1.094880e-01 8 70403700 70403837 138 - 0.855 0.663 -0.775