ENSG00000140391

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000346495 ENSG00000140391 HEK293_OSMI2_2hA HEK293_TMG_2hB TSPAN3 protein_coding protein_coding 323.1653 285.7544 361.1073 29.11198 1.156847 0.3376415 72.54363 80.900972 63.07520 7.489264 3.267715 -0.3590338 0.23065417 0.2839333 0.1746333 -0.10930000 6.747001e-08 6.747001e-08 FALSE TRUE
ENST00000558394 ENSG00000140391 HEK293_OSMI2_2hA HEK293_TMG_2hB TSPAN3 protein_coding processed_transcript 323.1653 285.7544 361.1073 29.11198 1.156847 0.3376415 32.51911 8.746855 66.36318 3.186339 5.648342 2.9221155 0.09418333 0.0316000 0.1838000 0.15220000 1.089603e-04 6.747001e-08 FALSE FALSE
ENST00000561277 ENSG00000140391 HEK293_OSMI2_2hA HEK293_TMG_2hB TSPAN3 protein_coding protein_coding 323.1653 285.7544 361.1073 29.11198 1.156847 0.3376415 205.43603 183.402885 214.93012 21.287381 5.130372 0.2288398 0.63571667 0.6400667 0.5952000 -0.04486667 2.477139e-01 6.747001e-08   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000140391 E001 454.482210 1.433452e-04 8.852555e-02 1.728694e-01 15 77041404 77046029 4626 - 2.672 2.655 -0.056
ENSG00000140391 E002 186.707596 2.500185e-03 5.988149e-09 7.710561e-08 15 77046030 77046059 30 - 2.139 2.362 0.743
ENSG00000140391 E003 345.111305 8.717071e-03 1.025915e-02 2.940429e-02 15 77046060 77046083 24 - 2.458 2.593 0.450
ENSG00000140391 E004 2549.332500 3.280478e-03 4.421877e-03 1.432358e-02 15 77046084 77046331 248 - 3.357 3.439 0.272
ENSG00000140391 E005 6147.690128 8.355542e-04 1.647718e-05 1.041698e-04 15 77046332 77046927 596 - 3.756 3.810 0.181
ENSG00000140391 E006 1658.031051 4.286164e-04 9.668503e-08 9.843978e-07 15 77052385 77052468 84 - 3.177 3.254 0.256
ENSG00000140391 E007 2218.681375 3.437299e-04 2.783196e-06 2.098746e-05 15 77052777 77052929 153 - 3.313 3.373 0.200
ENSG00000140391 E008 976.025323 1.241074e-04 5.161552e-01 6.507201e-01 15 77054178 77054180 3 - 2.982 3.002 0.064
ENSG00000140391 E009 2141.143973 9.122651e-05 5.860410e-02 1.242362e-01 15 77054181 77054279 99 - 3.336 3.330 -0.019
ENSG00000140391 E010 18.362840 9.760168e-04 4.188787e-05 2.402984e-04 15 77054280 77055788 1509 - 1.434 1.119 -1.107
ENSG00000140391 E011 1521.271433 1.825238e-04 2.014843e-04 9.701266e-04 15 77055789 77055829 41 - 3.201 3.170 -0.103
ENSG00000140391 E012 1160.292695 8.041804e-04 6.985762e-07 6.000053e-06 15 77055830 77055835 6 - 3.107 3.028 -0.261
ENSG00000140391 E013 1434.689877 5.981120e-04 2.341053e-06 1.794261e-05 15 77055836 77055863 28 - 3.191 3.127 -0.213
ENSG00000140391 E014 6.101008 1.277430e-02 1.724875e-01 2.914306e-01 15 77055864 77056063 200 - 0.936 0.763 -0.669
ENSG00000140391 E015 2518.400147 8.985903e-04 8.393259e-13 2.086990e-11 15 77056064 77056255 192 - 3.455 3.350 -0.351
ENSG00000140391 E016 15.483745 1.124768e-03 2.983280e-07 2.762738e-06 15 77069867 77070058 192 - 1.407 0.969 -1.561
ENSG00000140391 E017 1025.813197 4.906808e-03 4.052661e-04 1.795017e-03 15 77070892 77071004 113 - 3.075 2.948 -0.423
ENSG00000140391 E018 527.928579 1.031437e-02 1.152182e-03 4.478406e-03 15 77071005 77071122 118 - 2.809 2.635 -0.577
ENSG00000140391 E019 1.180092 3.564217e-01 7.865327e-01 8.632990e-01 15 77083847 77083984 138 - 0.366 0.344 -0.129