ENSG00000140265

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000396976 ENSG00000140265 HEK293_OSMI2_2hA HEK293_TMG_2hB ZSCAN29 protein_coding protein_coding 6.298572 3.671627 8.158821 0.2692469 0.4078626 1.149784 1.4028633 1.41406259 1.4166612 0.15341021 0.3339739 0.00263018 0.25134167 0.38390000 0.1705333 -0.21336667 0.009827637 0.009827637 FALSE TRUE
ENST00000566849 ENSG00000140265 HEK293_OSMI2_2hA HEK293_TMG_2hB ZSCAN29 protein_coding nonsense_mediated_decay 6.298572 3.671627 8.158821 0.2692469 0.4078626 1.149784 0.4654286 0.03028836 0.6536704 0.01835445 0.2167036 4.04203188 0.05747083 0.00810000 0.0785000 0.07040000 0.019004685 0.009827637 FALSE TRUE
ENST00000684362 ENSG00000140265 HEK293_OSMI2_2hA HEK293_TMG_2hB ZSCAN29 protein_coding protein_coding 6.298572 3.671627 8.158821 0.2692469 0.4078626 1.149784 3.7897760 1.82604592 5.4234495 0.13885945 0.1577664 1.56526625 0.59297083 0.50663333 0.6697667 0.16313333 0.255543239 0.009827637 FALSE TRUE
MSTRG.10658.1 ENSG00000140265 HEK293_OSMI2_2hA HEK293_TMG_2hB ZSCAN29 protein_coding   6.298572 3.671627 8.158821 0.2692469 0.4078626 1.149784 0.1091725 0.26790018 0.0000000 0.16252724 0.0000000 -4.79649486 0.02316250 0.06733333 0.0000000 -0.06733333 0.044851818 0.009827637 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000140265 E001 0.6234634 0.0176444663 9.760010e-02 1.868266e-01 15 43358096 43358171 76 - 0.087 0.386 2.692
ENSG00000140265 E002 1.0694743 0.0121989077 6.470687e-04 2.705658e-03 15 43358172 43358174 3 - 0.000 0.587 12.898
ENSG00000140265 E003 11.5664895 0.0023342628 3.895131e-04 1.734091e-03 15 43358175 43358220 46 - 0.908 1.293 1.394
ENSG00000140265 E004 414.4262629 0.0032393553 6.272729e-13 1.590829e-11 15 43358221 43360917 2697 - 2.519 2.721 0.674
ENSG00000140265 E005 35.8169456 0.0043900501 6.900480e-02 1.417239e-01 15 43360918 43361017 100 - 1.577 1.493 -0.287
ENSG00000140265 E006 30.5896373 0.0159183139 1.730905e-01 2.921969e-01 15 43361018 43361072 55 - 1.508 1.423 -0.291
ENSG00000140265 E007 28.7900807 0.0009260368 2.356271e-01 3.691090e-01 15 43361073 43361161 89 - 1.468 1.429 -0.135
ENSG00000140265 E008 146.5732391 0.0019349639 6.559499e-01 7.657334e-01 15 43361162 43361941 780 - 2.142 2.168 0.086
ENSG00000140265 E009 0.6558305 0.0883345636 5.262489e-01 6.594934e-01 15 43363749 43363914 166 - 0.159 0.292 1.114
ENSG00000140265 E010 52.4290652 0.0004287277 8.561012e-01 9.110508e-01 15 43363915 43364125 211 - 1.693 1.744 0.174
ENSG00000140265 E011 52.7441280 0.0011096083 1.644110e-02 4.370428e-02 15 43364126 43364382 257 - 1.744 1.662 -0.279
ENSG00000140265 E012 3.9753964 0.0709630541 3.705594e-01 5.149823e-01 15 43364383 43364487 105 - 0.734 0.586 -0.632
ENSG00000140265 E013 114.6315075 0.0054184227 4.047717e-03 1.327473e-02 15 43366110 43366774 665 - 2.076 1.980 -0.322
ENSG00000140265 E014 26.4682306 0.0046686045 1.538467e-01 2.668992e-01 15 43366775 43366808 34 - 1.441 1.371 -0.240
ENSG00000140265 E015 45.4324195 0.0004559563 1.198176e-02 3.348838e-02 15 43368923 43369127 205 - 1.683 1.588 -0.321
ENSG00000140265 E016 66.3040579 0.0004275397 1.304346e-05 8.432447e-05 15 43369596 43369997 402 - 1.863 1.705 -0.535
ENSG00000140265 E017 19.9567079 0.0009104610 5.875629e-07 5.128878e-06 15 43369998 43370025 28 - 1.412 1.004 -1.450
ENSG00000140265 E018 14.0790580 0.0327669324 2.495703e-01 3.854145e-01 15 43370026 43370348 323 - 1.205 1.092 -0.403
ENSG00000140265 E019 42.1581532 0.0010313152 8.359725e-06 5.653535e-05 15 43370558 43371043 486 - 1.685 1.458 -0.773