ENSG00000140044

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000267569 ENSG00000140044 HEK293_OSMI2_2hA HEK293_TMG_2hB JDP2 protein_coding protein_coding 0.9192291 1.144725 1.112599 0.09371047 0.04871634 -0.0407061 0.14050867 0.17968277 0.13266750 0.09347825 0.08278783 -0.4109319 0.18771667 0.14970000 0.12330000 -0.02640000 8.804900e-01 1.411733e-06 FALSE TRUE
ENST00000419727 ENSG00000140044 HEK293_OSMI2_2hA HEK293_TMG_2hB JDP2 protein_coding protein_coding 0.9192291 1.144725 1.112599 0.09371047 0.04871634 -0.0407061 0.05399111 0.00000000 0.00000000 0.00000000 0.00000000 0.0000000 0.08281250 0.00000000 0.00000000 0.00000000   1.411733e-06 FALSE TRUE
ENST00000435893 ENSG00000140044 HEK293_OSMI2_2hA HEK293_TMG_2hB JDP2 protein_coding protein_coding 0.9192291 1.144725 1.112599 0.09371047 0.04871634 -0.0407061 0.01746871 0.09317427 0.00000000 0.09317427 0.00000000 -3.3670113 0.01382083 0.07236667 0.00000000 -0.07236667 7.508985e-01 1.411733e-06 FALSE TRUE
ENST00000437176 ENSG00000140044 HEK293_OSMI2_2hA HEK293_TMG_2hB JDP2 protein_coding protein_coding 0.9192291 1.144725 1.112599 0.09371047 0.04871634 -0.0407061 0.14995463 0.00000000 0.00000000 0.00000000 0.00000000 0.0000000 0.15352917 0.00000000 0.00000000 0.00000000   1.411733e-06 FALSE TRUE
ENST00000558068 ENSG00000140044 HEK293_OSMI2_2hA HEK293_TMG_2hB JDP2 protein_coding processed_transcript 0.9192291 1.144725 1.112599 0.09371047 0.04871634 -0.0407061 0.12215876 0.03755645 0.15898546 0.03755645 0.15898546 1.8291863 0.11965000 0.02916667 0.14586667 0.11670000 1.000000e+00 1.411733e-06 FALSE FALSE
ENST00000559060 ENSG00000140044 HEK293_OSMI2_2hA HEK293_TMG_2hB JDP2 protein_coding protein_coding 0.9192291 1.144725 1.112599 0.09371047 0.04871634 -0.0407061 0.10941985 0.50546127 0.00000000 0.08502968 0.00000000 -5.6877921 0.13863750 0.45550000 0.00000000 -0.45550000 1.411733e-06 1.411733e-06 FALSE TRUE
ENST00000559773 ENSG00000140044 HEK293_OSMI2_2hA HEK293_TMG_2hB JDP2 protein_coding processed_transcript 0.9192291 1.144725 1.112599 0.09371047 0.04871634 -0.0407061 0.10860661 0.02782382 0.53027424 0.02782382 0.15879099 3.8363248 0.08884583 0.02363333 0.47180000 0.44816667 6.331570e-03 1.411733e-06   FALSE
ENST00000651602 ENSG00000140044 HEK293_OSMI2_2hA HEK293_TMG_2hB JDP2 protein_coding protein_coding 0.9192291 1.144725 1.112599 0.09371047 0.04871634 -0.0407061 0.08722334 0.00000000 0.05701440 0.00000000 0.05701440 2.7444712 0.07081667 0.00000000 0.04726667 0.04726667 7.694669e-01 1.411733e-06 FALSE TRUE
MSTRG.9926.10 ENSG00000140044 HEK293_OSMI2_2hA HEK293_TMG_2hB JDP2 protein_coding   0.9192291 1.144725 1.112599 0.09371047 0.04871634 -0.0407061 0.05272959 0.22413791 0.01546819 0.22413791 0.01546819 -3.2005904 0.05542917 0.19026667 0.01283333 -0.17743333 9.182825e-01 1.411733e-06 FALSE TRUE
MSTRG.9926.8 ENSG00000140044 HEK293_OSMI2_2hA HEK293_TMG_2hB JDP2 protein_coding   0.9192291 1.144725 1.112599 0.09371047 0.04871634 -0.0407061 0.07716778 0.07688830 0.21818941 0.07688830 0.07380843 1.3929980 0.08875000 0.07940000 0.19890000 0.11950000 3.919109e-01 1.411733e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000140044 E001 0.0000000       14 75427716 75427805 90 +      
ENSG00000140044 E002 0.3030308 0.4199930891 1.063650e-01   14 75427806 75427849 44 + 0.308 0.000 -11.721
ENSG00000140044 E003 1.0611596 0.0144998638 2.070279e-03 7.442420e-03 14 75428022 75428045 24 + 0.611 0.081 -3.900
ENSG00000140044 E004 0.9159869 0.0144205461 6.376469e-03 1.960089e-02 14 75428046 75428095 50 + 0.552 0.081 -3.638
ENSG00000140044 E005 2.1714320 0.0071574679 4.752980e-01 6.140131e-01 14 75428096 75428216 121 + 0.552 0.423 -0.638
ENSG00000140044 E006 2.4754925 0.0127452618 9.576469e-01 9.774490e-01 14 75428217 75428252 36 + 0.485 0.487 0.008
ENSG00000140044 E007 0.3634088 0.3199724745 6.554303e-01   14 75428357 75428606 250 + 0.000 0.151 10.093
ENSG00000140044 E008 1.4341202 0.0108782012 2.859034e-03 9.850423e-03 14 75431826 75431901 76 + 0.662 0.150 -3.122
ENSG00000140044 E009 1.8164554 0.1105849911 3.391878e-01 4.831185e-01 14 75432134 75432336 203 + 0.553 0.350 -1.058
ENSG00000140044 E010 0.2965864 0.1623637065 6.235620e-01   14 75436335 75436409 75 + 0.000 0.148 10.260
ENSG00000140044 E011 21.7211980 0.0011234556 5.109318e-02 1.111201e-01 14 75437898 75438121 224 + 1.400 1.248 -0.530
ENSG00000140044 E012 2.6979764 0.0056888070 1.930548e-06 1.506576e-05 14 75445090 75445379 290 + 0.940 0.209 -3.639
ENSG00000140044 E013 24.8317378 0.0011612881 8.693413e-01 9.200171e-01 14 75461426 75461530 105 + 1.353 1.355 0.005
ENSG00000140044 E014 97.0329048 0.0004922366 1.356035e-06 1.094622e-05 14 75469290 75474111 4822 + 1.841 1.960 0.402