ENSG00000138814

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000323055 ENSG00000138814 HEK293_OSMI2_2hA HEK293_TMG_2hB PPP3CA protein_coding protein_coding 10.15294 5.267361 18.65725 0.2648805 0.4537851 1.822621 3.092790 2.49773931 4.050195 0.29507361 0.2928375 0.6951615 0.3717333 0.47090000 0.2180667 -0.2528333 1.828470e-05 5.005382e-16 FALSE TRUE
ENST00000394853 ENSG00000138814 HEK293_OSMI2_2hA HEK293_TMG_2hB PPP3CA protein_coding protein_coding 10.15294 5.267361 18.65725 0.2648805 0.4537851 1.822621 1.802946 0.06440032 4.916489 0.04825501 0.2622360 6.0491072 0.1317000 0.01313333 0.2635000 0.2503667 5.005382e-16 5.005382e-16 FALSE TRUE
ENST00000394854 ENSG00000138814 HEK293_OSMI2_2hA HEK293_TMG_2hB PPP3CA protein_coding protein_coding 10.15294 5.267361 18.65725 0.2648805 0.4537851 1.822621 2.489254 2.57876507 4.475038 0.09930931 0.4075605 0.7928563 0.2953333 0.49130000 0.2399667 -0.2513333 1.103088e-05 5.005382e-16 FALSE TRUE
MSTRG.25235.5 ENSG00000138814 HEK293_OSMI2_2hA HEK293_TMG_2hB PPP3CA protein_coding   10.15294 5.267361 18.65725 0.2648805 0.4537851 1.822621 2.424023 0.07921360 4.165351 0.07921360 0.3835185 5.5484900 0.1721000 0.01476667 0.2226000 0.2078333 5.049536e-03 5.005382e-16 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000138814 E001 0.0000000       4 101023409 101023417 9 -      
ENSG00000138814 E002 0.2214452 0.0403678145 1.512263e-01   4 101023418 101023430 13 - 0.000 0.205 12.412
ENSG00000138814 E003 238.9437165 0.0061464194 1.543804e-27 2.111689e-25 4 101023431 101024327 897 - 2.133 2.629 1.656
ENSG00000138814 E004 277.8890522 0.0022604009 7.095244e-25 7.690959e-23 4 101024328 101025155 828 - 2.270 2.617 1.158
ENSG00000138814 E005 70.8349769 0.0003808116 1.395749e-03 5.292750e-03 4 101025156 101025317 162 - 1.745 1.939 0.656
ENSG00000138814 E006 233.6530121 0.0002530057 3.961238e-01 5.399077e-01 4 101025318 101026061 744 - 2.307 2.339 0.104
ENSG00000138814 E007 1.0404339 0.0118751368 4.989615e-01 6.353417e-01 4 101029166 101029195 30 - 0.313 0.204 -0.814
ENSG00000138814 E008 75.3571137 0.0003222071 4.733195e-02 1.044204e-01 4 101032267 101032364 98 - 1.839 1.799 -0.134
ENSG00000138814 E009 82.7658234 0.0003050788 6.330135e-02 1.322101e-01 4 101040482 101040566 85 - 1.876 1.846 -0.100
ENSG00000138814 E010 84.2742130 0.0003557341 2.200591e-01 3.504765e-01 4 101061087 101061161 75 - 1.876 1.875 -0.003
ENSG00000138814 E011 93.3343406 0.0003491268 3.057629e-04 1.402869e-03 4 101063232 101063357 126 - 1.945 1.842 -0.346
ENSG00000138814 E012 76.0382863 0.0003440464 1.727363e-03 6.368965e-03 4 101080532 101080626 95 - 1.856 1.760 -0.324
ENSG00000138814 E013 37.7008276 0.0006468958 5.253027e-03 1.661996e-02 4 101083186 101083194 9 - 1.567 1.429 -0.474
ENSG00000138814 E014 53.3211334 0.0004465180 3.898248e-05 2.253070e-04 4 101083195 101083263 69 - 1.722 1.533 -0.646
ENSG00000138814 E015 67.0416868 0.0003802013 3.537015e-04 1.594487e-03 4 101093776 101093867 92 - 1.806 1.675 -0.444
ENSG00000138814 E016 61.7862515 0.0003921835 1.339200e-03 5.107254e-03 4 101093868 101093915 48 - 1.770 1.652 -0.399
ENSG00000138814 E017 61.3816975 0.0003929631 2.252401e-05 1.380136e-04 4 101098367 101098417 51 - 1.782 1.603 -0.606
ENSG00000138814 E018 61.6867421 0.0006037887 6.867974e-06 4.738744e-05 4 101098418 101098462 45 - 1.787 1.590 -0.668
ENSG00000138814 E019 55.6520607 0.0004089546 1.833790e-05 1.147426e-04 4 101098463 101098512 50 - 1.742 1.548 -0.661
ENSG00000138814 E020 73.8236150 0.0003427383 1.399914e-04 7.035480e-04 4 101099611 101099722 112 - 1.851 1.717 -0.451
ENSG00000138814 E021 0.4375944 0.0249565222 4.426801e-01 5.842623e-01 4 101108074 101108161 88 - 0.184 0.000 -10.739
ENSG00000138814 E022 93.8137526 0.0020210390 7.026802e-06 4.838112e-05 4 101108954 101109078 125 - 1.958 1.791 -0.562
ENSG00000138814 E023 0.0000000       4 101158170 101158319 150 -      
ENSG00000138814 E024 0.9027498 0.4249188766 3.117886e-01 4.542984e-01 4 101171084 101171226 143 - 0.315 0.000 -11.750
ENSG00000138814 E025 114.7622417 0.0050379408 1.300279e-08 1.569506e-07 4 101195916 101196116 201 - 2.060 1.805 -0.859
ENSG00000138814 E026 89.0462746 0.0115232437 5.628929e-06 3.960424e-05 4 101346739 101347041 303 - 1.954 1.679 -0.925
ENSG00000138814 E027 40.6263905 0.0043158630 1.335479e-05 8.616663e-05 4 101347042 101347288 247 - 1.620 1.333 -0.987
ENSG00000138814 E028 22.9834599 0.0008587531 2.473093e-03 8.682891e-03 4 101347289 101347479 191 - 1.372 1.153 -0.768
ENSG00000138814 E029 8.6265449 0.0020080482 3.602517e-01 5.045734e-01 4 101347480 101347560 81 - 0.950 0.882 -0.260
ENSG00000138814 E030 0.0000000       4 101348233 101348278 46 -