ENSG00000138777

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000351450 ENSG00000138777 HEK293_OSMI2_2hA HEK293_TMG_2hB PPA2 protein_coding nonsense_mediated_decay 87.14239 57.36243 120.976 8.578785 3.069295 1.076411 11.071011 10.599672 13.046385 2.0513237 1.0486169 0.2993754 0.12350000 0.18260000 0.10753333 -0.075066667 2.348478e-05 3.224332e-06 FALSE TRUE
ENST00000457404 ENSG00000138777 HEK293_OSMI2_2hA HEK293_TMG_2hB PPA2 protein_coding processed_transcript 87.14239 57.36243 120.976 8.578785 3.069295 1.076411 12.228399 1.145821 19.908084 1.1458212 0.4839930 4.1070887 0.10115833 0.01540000 0.16493333 0.149533333 3.797630e-02 3.224332e-06 FALSE FALSE
ENST00000499847 ENSG00000138777 HEK293_OSMI2_2hA HEK293_TMG_2hB PPA2 protein_coding retained_intron 87.14239 57.36243 120.976 8.578785 3.069295 1.076411 8.330839 11.028442 8.966950 1.4160206 1.2681003 -0.2982393 0.12370833 0.19416667 0.07380000 -0.120366667 3.224332e-06 3.224332e-06 FALSE TRUE
ENST00000505713 ENSG00000138777 HEK293_OSMI2_2hA HEK293_TMG_2hB PPA2 protein_coding processed_transcript 87.14239 57.36243 120.976 8.578785 3.069295 1.076411 3.604974 2.764818 4.896507 0.2804679 0.2259607 0.8223028 0.05195833 0.05026667 0.04060000 -0.009666667 7.318629e-01 3.224332e-06 FALSE FALSE
MSTRG.25266.10 ENSG00000138777 HEK293_OSMI2_2hA HEK293_TMG_2hB PPA2 protein_coding   87.14239 57.36243 120.976 8.578785 3.069295 1.076411 24.254200 12.581051 33.930252 2.0139536 1.1526414 1.4305985 0.26752500 0.21873333 0.28130000 0.062566667 5.521076e-02 3.224332e-06 FALSE TRUE
MSTRG.25266.19 ENSG00000138777 HEK293_OSMI2_2hA HEK293_TMG_2hB PPA2 protein_coding   87.14239 57.36243 120.976 8.578785 3.069295 1.076411 6.207643 3.579958 10.649597 0.3168422 0.6233349 1.5701141 0.07416667 0.06556667 0.08813333 0.022566667 4.337122e-01 3.224332e-06 FALSE TRUE
MSTRG.25266.3 ENSG00000138777 HEK293_OSMI2_2hA HEK293_TMG_2hB PPA2 protein_coding   87.14239 57.36243 120.976 8.578785 3.069295 1.076411 5.939444 4.228059 6.319827 2.3451400 2.2204836 0.5787622 0.06969583 0.06623333 0.05136667 -0.014866667 9.838816e-01 3.224332e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000138777 E001 3.3077037 0.0529186837 3.657548e-02 8.466661e-02 4 105369077 105369242 166 - 0.435 0.794 1.600
ENSG00000138777 E002 8.7411204 0.0235724038 2.248083e-04 1.069571e-03 4 105369243 105369520 278 - 0.715 1.196 1.812
ENSG00000138777 E003 5.5096944 0.0030771052 1.383785e-01 2.458351e-01 4 105369521 105369560 40 - 0.715 0.920 0.806
ENSG00000138777 E004 4.8786551 0.0033638233 1.146119e-01 2.122678e-01 4 105369561 105369564 4 - 0.671 0.896 0.898
ENSG00000138777 E005 10.2928755 0.0198123796 2.120267e-01 3.409137e-01 4 105369565 105369579 15 - 0.983 1.162 0.651
ENSG00000138777 E006 398.3282612 0.0052438883 2.010296e-03 7.258055e-03 4 105369580 105369753 174 - 2.536 2.675 0.462
ENSG00000138777 E007 299.6261794 0.0039334410 1.915947e-03 6.964930e-03 4 105370837 105370873 37 - 2.417 2.550 0.443
ENSG00000138777 E008 321.4374889 0.0029652854 1.513603e-04 7.531320e-04 4 105386567 105386612 46 - 2.444 2.586 0.473
ENSG00000138777 E009 234.7514006 0.0029461632 7.003887e-03 2.123358e-02 4 105386613 105386636 24 - 2.323 2.435 0.374
ENSG00000138777 E010 389.1620478 0.0001959269 7.066062e-09 8.959536e-08 4 105396249 105396325 77 - 2.538 2.661 0.412
ENSG00000138777 E011 239.2255473 0.0004501283 7.274636e-05 3.933997e-04 4 105396326 105396334 9 - 2.331 2.443 0.377
ENSG00000138777 E012 8.1346085 0.0021247428 3.334804e-01 4.772088e-01 4 105398304 105398867 564 - 0.994 0.897 -0.364
ENSG00000138777 E013 355.6467291 0.0010419068 4.156079e-02 9.392858e-02 4 105399037 105399078 42 - 2.525 2.586 0.204
ENSG00000138777 E014 427.9451372 0.0004236103 3.743389e-02 8.628362e-02 4 105399079 105399150 72 - 2.608 2.660 0.176
ENSG00000138777 E015 261.6012952 0.0002308580 7.293964e-01 8.215825e-01 4 105399151 105399164 14 - 2.407 2.430 0.078
ENSG00000138777 E016 5.3198505 0.1738009156 7.025714e-01 8.013827e-01 4 105399165 105399636 472 - 0.756 0.858 0.402
ENSG00000138777 E017 11.3206897 0.0149235612 5.408764e-01 6.719483e-01 4 105405637 105405837 201 - 1.116 1.067 -0.177
ENSG00000138777 E018 8.2304529 0.0037606758 2.057666e-02 5.270354e-02 4 105405838 105405948 111 - 1.054 0.790 -1.002
ENSG00000138777 E019 1.3639869 0.0100023125 2.797688e-01 4.195435e-01 4 105422155 105422155 1 - 0.298 0.498 1.125
ENSG00000138777 E020 23.2902195 0.0011892934 1.572488e-07 1.537803e-06 4 105422156 105422433 278 - 1.509 1.101 -1.429
ENSG00000138777 E021 10.8396182 0.0016728020 3.150537e-01 4.578128e-01 4 105423204 105423381 178 - 1.108 1.020 -0.320
ENSG00000138777 E022 425.4507719 0.0001457616 1.696903e-03 6.273513e-03 4 105424196 105424248 53 - 2.644 2.603 -0.135
ENSG00000138777 E023 462.2785765 0.0001632011 2.700523e-04 1.257883e-03 4 105424249 105424322 74 - 2.683 2.636 -0.156
ENSG00000138777 E024 291.7031481 0.0015702470 2.795248e-02 6.792256e-02 4 105437950 105437976 27 - 2.483 2.436 -0.155
ENSG00000138777 E025 357.2673344 0.0003482514 8.400343e-05 4.470280e-04 4 105437977 105438014 38 - 2.578 2.512 -0.219
ENSG00000138777 E026 312.3894023 0.0002167913 7.489321e-06 5.119355e-05 4 105438015 105438036 22 - 2.525 2.446 -0.263
ENSG00000138777 E027 35.4572484 0.0009335137 9.452017e-14 2.697178e-12 4 105446202 105446382 181 - 1.321 1.780 1.572
ENSG00000138777 E028 514.3525274 0.0002116001 6.251285e-03 1.927331e-02 4 105446383 105446502 120 - 2.723 2.693 -0.100
ENSG00000138777 E029 0.2966881 0.0270525199 4.060248e-01   4 105447995 105448093 99 - 0.174 0.000 -9.984
ENSG00000138777 E030 329.3283584 0.0002377265 1.876429e-03 6.842148e-03 4 105449350 105449403 54 - 2.537 2.488 -0.163
ENSG00000138777 E031 331.4266719 0.0001721456 1.511178e-06 1.207458e-05 4 105453598 105453642 45 - 2.551 2.469 -0.275
ENSG00000138777 E032 0.1451727 0.0444842071 8.598566e-01   4 105453781 105453908 128 - 0.096 0.000 -8.983
ENSG00000138777 E033 0.3289534 0.0360522488 6.963244e-01   4 105456081 105456247 167 - 0.096 0.156 0.807
ENSG00000138777 E034 3.9889858 0.0088326773 1.773840e-01 2.978469e-01 4 105456248 105456291 44 - 0.754 0.555 -0.854
ENSG00000138777 E035 0.8952612 0.0140730478 2.762086e-01 4.156325e-01 4 105456292 105456680 389 - 0.348 0.155 -1.517
ENSG00000138777 E036 237.0559048 0.0002141517 1.776544e-08 2.091090e-07 4 105456681 105456695 15 - 2.419 2.297 -0.406
ENSG00000138777 E037 293.7595358 0.0001703886 1.005876e-07 1.020512e-06 4 105456696 105456745 50 - 2.505 2.405 -0.333
ENSG00000138777 E038 100.4259257 0.0398284694 9.314433e-01 9.608134e-01 4 105473145 105473339 195 - 1.996 2.000 0.014
ENSG00000138777 E039 46.3432127 0.0331563619 4.195479e-01 5.628007e-01 4 105473340 105473357 18 - 1.631 1.713 0.278
ENSG00000138777 E040 39.5525516 0.0238220513 4.939471e-01 6.309711e-01 4 105473358 105473378 21 - 1.575 1.635 0.204
ENSG00000138777 E041 93.2275592 0.0155206436 6.381771e-01 7.518796e-01 4 105473379 105473775 397 - 1.957 1.982 0.084
ENSG00000138777 E042 293.3274978 0.0025045368 1.309659e-01 2.354866e-01 4 105473894 105474076 183 - 2.482 2.439 -0.141