ENSG00000138750

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000264883 ENSG00000138750 HEK293_OSMI2_2hA HEK293_TMG_2hB NUP54 protein_coding protein_coding 34.39039 10.66101 61.76001 0.9890248 0.1956957 2.53321 16.9566810 7.0905421 32.112933 0.9411914 1.3565474 2.177603 0.52168750 0.6611667 0.51993333 -0.14123333 6.092336e-02 2.634409e-29 FALSE TRUE
ENST00000507257 ENSG00000138750 HEK293_OSMI2_2hA HEK293_TMG_2hB NUP54 protein_coding nonsense_mediated_decay 34.39039 10.66101 61.76001 0.9890248 0.1956957 2.53321 0.2870449 1.2596135 0.000000 0.2782758 0.0000000 -6.988246 0.02662500 0.1161667 0.00000000 -0.11616667 2.634409e-29 2.634409e-29 FALSE TRUE
ENST00000512151 ENSG00000138750 HEK293_OSMI2_2hA HEK293_TMG_2hB NUP54 protein_coding nonsense_mediated_decay 34.39039 10.66101 61.76001 0.9890248 0.1956957 2.53321 2.5040488 0.0000000 4.931158 0.0000000 0.8440298 8.948705 0.04617083 0.0000000 0.07976667 0.07976667 6.646197e-12 2.634409e-29 FALSE TRUE
ENST00000514987 ENSG00000138750 HEK293_OSMI2_2hA HEK293_TMG_2hB NUP54 protein_coding protein_coding 34.39039 10.66101 61.76001 0.9890248 0.1956957 2.53321 2.3155400 0.6474274 3.894471 0.3385080 0.5693977 2.570224 0.07499167 0.0636000 0.06310000 -0.00050000 8.918480e-01 2.634409e-29 FALSE TRUE
MSTRG.25082.20 ENSG00000138750 HEK293_OSMI2_2hA HEK293_TMG_2hB NUP54 protein_coding   34.39039 10.66101 61.76001 0.9890248 0.1956957 2.53321 4.9073939 0.0000000 9.108700 0.0000000 0.9800426 9.832684 0.09390833 0.0000000 0.14746667 0.14746667 1.302760e-20 2.634409e-29 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000138750 E001 0.5943067 0.0225012989 6.758871e-01 7.810132e-01 4 76107562 76112622 5061 - 0.158 0.237 0.728
ENSG00000138750 E002 11.0488282 0.0015598261 1.569066e-04 7.779706e-04 4 76112792 76114629 1838 - 0.870 1.282 1.499
ENSG00000138750 E003 0.7437457 0.0153787590 8.676055e-01 9.188474e-01 4 76114630 76114658 29 - 0.200 0.237 0.313
ENSG00000138750 E004 0.2965864 0.1132292211 1.806309e-02   4 76114659 76114663 5 - 0.000 0.386 12.674
ENSG00000138750 E005 0.2965864 0.1132292211 1.806309e-02   4 76114664 76114678 15 - 0.000 0.386 12.674
ENSG00000138750 E006 0.4438354 0.0700294245 6.449536e-02 1.342097e-01 4 76114679 76114691 13 - 0.059 0.387 3.303
ENSG00000138750 E007 0.5921286 0.2837181615 4.638105e-02 1.027288e-01 4 76114692 76114707 16 - 0.059 0.491 3.844
ENSG00000138750 E008 8.3503441 0.0186949825 3.375901e-09 4.539709e-08 4 76114708 76114743 36 - 0.609 1.356 2.822
ENSG00000138750 E009 30.2372539 0.0044682398 1.080123e-23 1.034510e-21 4 76114744 76114867 124 - 1.141 1.861 2.479
ENSG00000138750 E010 213.8975793 0.0041349788 1.054638e-13 2.986071e-12 4 76114868 76115086 219 - 2.153 2.464 1.039
ENSG00000138750 E011 97.4251580 0.0006257540 5.316564e-12 1.157177e-10 4 76115087 76115103 17 - 1.820 2.114 0.989
ENSG00000138750 E012 297.7464855 0.0031951164 3.204476e-05 1.892920e-04 4 76115104 76115300 197 - 2.341 2.500 0.530
ENSG00000138750 E013 188.3926746 0.0015338185 6.056923e-01 7.260512e-01 4 76115301 76115343 43 - 2.172 2.200 0.091
ENSG00000138750 E014 250.2928834 0.0009439414 1.832125e-01 3.052312e-01 4 76115344 76115437 94 - 2.290 2.340 0.165
ENSG00000138750 E015 204.8532117 0.0002652800 3.937305e-01 5.376204e-01 4 76115438 76115494 57 - 2.207 2.242 0.117
ENSG00000138750 E016 240.4105764 0.0002001941 4.416181e-02 9.868739e-02 4 76117664 76117742 79 - 2.270 2.335 0.218
ENSG00000138750 E017 176.7055047 0.0002310774 1.156040e-01 2.136979e-01 4 76117743 76117774 32 - 2.138 2.198 0.202
ENSG00000138750 E018 6.3379365 0.0027229825 9.892909e-01 9.973187e-01 4 76117775 76118074 300 - 0.775 0.784 0.035
ENSG00000138750 E019 287.9893876 0.0001809134 3.706570e-02 8.559535e-02 4 76118075 76118194 120 - 2.348 2.410 0.208
ENSG00000138750 E020 4.9291846 0.0141498197 2.384694e-01 3.723661e-01 4 76118195 76118437 243 - 0.639 0.834 0.793
ENSG00000138750 E021 1.3244365 0.0485303060 6.418637e-01 7.548101e-01 4 76124580 76124648 69 - 0.336 0.236 -0.688
ENSG00000138750 E022 229.4752196 0.0001606882 4.477791e-01 5.891328e-01 4 76124649 76124756 108 - 2.265 2.251 -0.048
ENSG00000138750 E023 1.9949125 0.0239993000 1.124537e-02 3.175040e-02 4 76129846 76129860 15 - 0.273 0.729 2.316
ENSG00000138750 E024 203.4018604 0.0001955689 5.683430e-03 1.777539e-02 4 76130656 76130718 63 - 2.226 2.143 -0.278
ENSG00000138750 E025 166.2721268 0.0001991202 6.984425e-04 2.893458e-03 4 76130719 76130749 31 - 2.144 2.026 -0.394
ENSG00000138750 E026 4.1866019 0.0039033485 1.267334e-01 2.296086e-01 4 76130750 76130792 43 - 0.681 0.389 -1.386
ENSG00000138750 E027 12.0303431 0.0019907679 8.680404e-02 1.702886e-01 4 76130793 76131229 437 - 1.055 0.833 -0.835
ENSG00000138750 E028 165.2667035 0.0003132751 1.244752e-01 2.263595e-01 4 76131230 76131284 55 - 2.132 2.084 -0.158
ENSG00000138750 E029 16.7373890 0.0010341757 4.870497e-01 6.247511e-01 4 76131285 76132522 1238 - 1.168 1.101 -0.240
ENSG00000138750 E030 276.2499296 0.0016241698 3.649496e-01 5.092859e-01 4 76132523 76132714 192 - 2.348 2.326 -0.072
ENSG00000138750 E031 102.1033390 0.0015830010 4.510595e-01 5.920630e-01 4 76132715 76132719 5 - 1.920 1.891 -0.097
ENSG00000138750 E032 247.7334657 0.0007574125 2.722666e-01 4.111245e-01 4 76134175 76134355 181 - 2.300 2.273 -0.089
ENSG00000138750 E033 94.6457256 0.0002909361 2.784712e-01 4.181405e-01 4 76134356 76134358 3 - 1.888 1.845 -0.146
ENSG00000138750 E034 92.9771860 0.0002848626 1.118647e-01 2.083060e-01 4 76134359 76134362 4 - 1.886 1.817 -0.232
ENSG00000138750 E035 92.0004439 0.0003189853 5.114563e-02 1.112074e-01 4 76136186 76136187 2 - 1.885 1.797 -0.295
ENSG00000138750 E036 180.0540113 0.0002149674 8.826187e-04 3.553188e-03 4 76136188 76136281 94 - 2.176 2.066 -0.369
ENSG00000138750 E037 117.9207958 0.0002874782 2.419588e-02 6.021303e-02 4 76136282 76136320 39 - 1.990 1.900 -0.303
ENSG00000138750 E038 171.2376850 0.0002364121 1.230252e-04 6.277406e-04 4 76136321 76136412 92 - 2.158 2.023 -0.450
ENSG00000138750 E039 171.3129178 0.0099598817 4.125529e-02 9.335065e-02 4 76144149 76144183 35 - 2.154 2.039 -0.387
ENSG00000138750 E040 140.6688901 0.0073559684 3.861999e-02 8.848251e-02 4 76144184 76144196 13 - 2.069 1.957 -0.376
ENSG00000138750 E041 219.4358911 0.0056443832 3.832391e-03 1.267187e-02 4 76144197 76144292 96 - 2.264 2.131 -0.447
ENSG00000138750 E042 209.8752993 0.0006100738 8.998828e-05 4.749411e-04 4 76144390 76144473 84 - 2.244 2.115 -0.432
ENSG00000138750 E043 5.4437340 0.0096167144 9.605942e-01 9.791964e-01 4 76144474 76145192 719 - 0.730 0.728 -0.008
ENSG00000138750 E044 5.4690929 0.0029498312 3.255053e-01 4.689435e-01 4 76145193 76145554 362 - 0.753 0.591 -0.685
ENSG00000138750 E045 1.9722147 0.0788644894 3.825851e-01 5.267940e-01 4 76145555 76145577 23 - 0.440 0.237 -1.272
ENSG00000138750 E046 4.8323037 0.1284157339 1.758014e-01 2.957242e-01 4 76145578 76145691 114 - 0.732 0.390 -1.593
ENSG00000138750 E047 5.9225950 0.0033201610 9.237423e-02 1.788180e-01 4 76145692 76145982 291 - 0.796 0.502 -1.270
ENSG00000138750 E048 6.1980769 0.0026420225 3.969026e-01 5.406696e-01 4 76145983 76146129 147 - 0.796 0.665 -0.533
ENSG00000138750 E049 2.8861373 0.0273804311 6.595429e-01 7.683444e-01 4 76146130 76146149 20 - 0.504 0.591 0.404
ENSG00000138750 E050 8.3117350 0.0582718696 8.138216e-01 8.819925e-01 4 76146841 76147478 638 - 0.886 0.837 -0.190
ENSG00000138750 E051 6.3065922 0.0221331844 2.352290e-02 5.882650e-02 4 76147479 76147640 162 - 0.835 0.390 -2.004
ENSG00000138750 E052 5.2652399 0.0050445381 4.711448e-02 1.040291e-01 4 76147641 76147755 115 - 0.764 0.390 -1.729
ENSG00000138750 E053 7.6727452 0.0428578217 4.378304e-02 9.799354e-02 4 76147756 76148089 334 - 0.903 0.503 -1.678
ENSG00000138750 E054 8.2768312 0.0019586410 1.000726e-02 2.877943e-02 4 76148090 76148307 218 - 0.934 0.502 -1.801
ENSG00000138750 E055 159.7525719 0.0002714372 3.232862e-05 1.907665e-04 4 76148308 76148485 178 - 2.130 1.976 -0.516