ENSG00000138738

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000264808 ENSG00000138738 HEK293_OSMI2_2hA HEK293_TMG_2hB PRDM5 protein_coding protein_coding 3.384221 2.456093 5.420257 0.2483484 0.3502045 1.138793 0.4050801 0.1017933 0.6271843 0.02262461 0.1063963 2.51087693 0.1148083 0.04113333 0.1168000 0.07566667 0.095973884 0.008906366 FALSE TRUE
ENST00000394435 ENSG00000138738 HEK293_OSMI2_2hA HEK293_TMG_2hB PRDM5 protein_coding protein_coding 3.384221 2.456093 5.420257 0.2483484 0.3502045 1.138793 0.5181526 0.2555463 0.9406917 0.02605499 0.1328141 1.84001436 0.1446042 0.10776667 0.1720667 0.06430000 0.262171999 0.008906366 FALSE FALSE
ENST00000512845 ENSG00000138738 HEK293_OSMI2_2hA HEK293_TMG_2hB PRDM5 protein_coding retained_intron 3.384221 2.456093 5.420257 0.2483484 0.3502045 1.138793 0.5253261 0.2273636 1.0393657 0.04809525 0.1444481 2.14434698 0.1350625 0.09723333 0.1965000 0.09926667 0.275341204 0.008906366 FALSE TRUE
MSTRG.25390.14 ENSG00000138738 HEK293_OSMI2_2hA HEK293_TMG_2hB PRDM5 protein_coding   3.384221 2.456093 5.420257 0.2483484 0.3502045 1.138793 1.2629597 1.6655997 1.5790096 0.29589357 0.4764621 -0.07654968 0.4315917 0.66850000 0.2848333 -0.38366667 0.008906366 0.008906366 TRUE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000138738 E001 0.0000000       4 120684919 120685025 107 -      
ENSG00000138738 E002 28.1006756 0.0450459693 2.084563e-01 3.366186e-01 4 120691915 120694822 2908 - 1.453 1.276 -0.615
ENSG00000138738 E003 4.9928018 0.0039294569 2.781597e-01 4.177925e-01 4 120694823 120695110 288 - 0.782 0.595 -0.786
ENSG00000138738 E004 2.5079548 0.0061159791 5.952262e-03 1.848284e-02 4 120695111 120695157 47 - 0.610 0.000 -12.920
ENSG00000138738 E005 3.6224711 0.0042755006 6.247954e-02 1.308167e-01 4 120695158 120695275 118 - 0.697 0.337 -1.760
ENSG00000138738 E006 3.1011155 0.0051764706 1.144754e-01 2.120793e-01 4 120710309 120710413 105 - 0.646 0.337 -1.549
ENSG00000138738 E007 0.2924217 0.0290785164 8.597028e-01   4 120731618 120731790 173 - 0.134 0.000 -10.423
ENSG00000138738 E008 2.4210706 0.0121989077 3.087994e-01 4.510669e-01 4 120754553 120754638 86 - 0.547 0.337 -1.107
ENSG00000138738 E009 0.1515154 0.0421590421 1.000000e+00   4 120762380 120762766 387 - 0.072 0.000 -9.492
ENSG00000138738 E010 2.9698734 0.0135107840 1.783754e-01 2.991006e-01 4 120777188 120777281 94 - 0.610 0.338 -1.385
ENSG00000138738 E011 3.5785630 0.0046276255 2.423328e-01 3.769229e-01 4 120781143 120781303 161 - 0.664 0.441 -1.036
ENSG00000138738 E012 1.8498310 0.0299676176 2.025763e-01 3.294317e-01 4 120784998 120785023 26 - 0.475 0.201 -1.757
ENSG00000138738 E013 2.2980345 0.0072356054 9.509107e-02 1.830132e-01 4 120785024 120785030 7 - 0.548 0.201 -2.107
ENSG00000138738 E014 3.5065569 0.0044893865 7.700043e-02 1.546875e-01 4 120785031 120785091 61 - 0.680 0.337 -1.692
ENSG00000138738 E015 3.8848307 0.0052477502 6.988869e-03 2.119484e-02 4 120798267 120798424 158 - 0.741 0.200 -2.944
ENSG00000138738 E016 1.9272640 0.0073940978 1.874141e-02 4.876665e-02 4 120799661 120799745 85 - 0.525 0.000 -12.638
ENSG00000138738 E017 0.3729606 0.0274060617 3.692623e-01 5.136819e-01 4 120807994 120808021 28 - 0.072 0.201 1.702
ENSG00000138738 E018 2.2938819 0.0064513110 9.491918e-02 1.827589e-01 4 120811370 120811449 80 - 0.548 0.201 -2.107
ENSG00000138738 E019 0.5933762 0.0219368802 3.358834e-01 4.797065e-01 4 120812551 120812814 264 - 0.236 0.000 -11.296
ENSG00000138738 E020 0.1515154 0.0421590421 1.000000e+00   4 120812815 120813006 192 - 0.072 0.000 -9.492
ENSG00000138738 E021 6.8831271 0.1594132629 9.725013e-01 9.868531e-01 4 120816063 120816452 390 - 0.854 0.884 0.117
ENSG00000138738 E022 10.4736633 0.0018466823 3.435498e-02 8.043908e-02 4 120816453 120816574 122 - 1.080 0.798 -1.062
ENSG00000138738 E023 4.3994496 0.0050289444 3.105184e-01 4.529248e-01 4 120816575 120816831 257 - 0.664 0.797 0.545
ENSG00000138738 E024 5.8994513 0.0200539379 4.959022e-01 6.326489e-01 4 120816832 120816924 93 - 0.831 0.707 -0.496
ENSG00000138738 E025 0.3666179 0.0281666312 3.686995e-01 5.130977e-01 4 120818171 120818352 182 - 0.072 0.201 1.703
ENSG00000138738 E026 14.9333316 0.0081333665 1.131273e-02 3.191129e-02 4 120818353 120818527 175 - 1.219 0.905 -1.142
ENSG00000138738 E027 17.5004921 0.0012273283 7.282963e-03 2.195912e-02 4 120821171 120821345 175 - 1.280 0.991 -1.037
ENSG00000138738 E028 0.0000000       4 120821346 120821348 3 -      
ENSG00000138738 E029 30.8833039 0.0013292609 8.152091e-01 8.829622e-01 4 120837345 120839274 1930 - 1.459 1.456 -0.013
ENSG00000138738 E030 17.6952156 0.0010066065 3.383152e-01 4.821706e-01 4 120853418 120853540 123 - 1.250 1.143 -0.380
ENSG00000138738 E031 11.2821112 0.0202617737 1.470227e-03 5.540130e-03 4 120881228 120881395 168 - 0.915 1.258 1.247
ENSG00000138738 E032 4.7460057 0.0037343067 3.529224e-02 8.221143e-02 4 120884548 120884622 75 - 0.628 0.905 1.114
ENSG00000138738 E033 0.2987644 0.0295337781 8.568972e-01   4 120885505 120885785 281 - 0.134 0.000 -10.423
ENSG00000138738 E034 29.6076749 0.0006883616 1.848017e-07 1.782593e-06 4 120907474 120907557 84 - 1.330 1.636 1.050
ENSG00000138738 E035 28.6928339 0.0006993184 2.289170e-07 2.170227e-06 4 120922516 120922870 355 - 1.316 1.624 1.061