ENSG00000138592

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000307179 ENSG00000138592 HEK293_OSMI2_2hA HEK293_TMG_2hB USP8 protein_coding protein_coding 14.08589 1.201736 26.21299 0.3083042 2.647615 4.435685 0.660712162 0.23067372 1.2943570 0.09644025 0.15670660 2.4381883 0.067766667 0.16730000 0.050533333 -0.11676667 6.692648e-03 9.96934e-07 FALSE TRUE
ENST00000396444 ENSG00000138592 HEK293_OSMI2_2hA HEK293_TMG_2hB USP8 protein_coding protein_coding 14.08589 1.201736 26.21299 0.3083042 2.647615 4.435685 0.140773776 0.07789944 0.1550474 0.07789944 0.15504737 0.9089543 0.046629167 0.13030000 0.005266667 -0.12503333 6.326487e-01 9.96934e-07 FALSE TRUE
ENST00000558091 ENSG00000138592 HEK293_OSMI2_2hA HEK293_TMG_2hB USP8 protein_coding protein_coding 14.08589 1.201736 26.21299 0.3083042 2.647615 4.435685 2.382928193 0.00000000 4.3451771 0.00000000 2.17271330 8.7665876 0.114525000 0.00000000 0.150733333 0.15073333 5.229601e-01 9.96934e-07 FALSE FALSE
ENST00000558892 ENSG00000138592 HEK293_OSMI2_2hA HEK293_TMG_2hB USP8 protein_coding processed_transcript 14.08589 1.201736 26.21299 0.3083042 2.647615 4.435685 1.234137057 0.07873451 2.0714436 0.07873451 0.08246879 4.5519455 0.053075000 0.04886667 0.080866667 0.03200000 6.897137e-01 9.96934e-07   FALSE
ENST00000559329 ENSG00000138592 HEK293_OSMI2_2hA HEK293_TMG_2hB USP8 protein_coding non_stop_decay 14.08589 1.201736 26.21299 0.3083042 2.647615 4.435685 2.032179864 0.43216467 3.8294203 0.24520577 0.76505344 3.1182328 0.195716667 0.29526667 0.156133333 -0.13913333 8.436047e-01 9.96934e-07 FALSE TRUE
ENST00000560954 ENSG00000138592 HEK293_OSMI2_2hA HEK293_TMG_2hB USP8 protein_coding processed_transcript 14.08589 1.201736 26.21299 0.3083042 2.647615 4.435685 0.653482417 0.00000000 1.9618793 0.00000000 0.29706207 7.6234274 0.025008333 0.00000000 0.079133333 0.07913333 1.426452e-02 9.96934e-07   FALSE
ENST00000561206 ENSG00000138592 HEK293_OSMI2_2hA HEK293_TMG_2hB USP8 protein_coding retained_intron 14.08589 1.201736 26.21299 0.3083042 2.647615 4.435685 2.693702769 0.00000000 5.4271620 0.00000000 0.69787455 9.0867100 0.158370833 0.00000000 0.208800000 0.20880000 9.969340e-07 9.96934e-07 FALSE FALSE
ENST00000625664 ENSG00000138592 HEK293_OSMI2_2hA HEK293_TMG_2hB USP8 protein_coding protein_coding 14.08589 1.201736 26.21299 0.3083042 2.647615 4.435685 3.084773717 0.13449911 6.0742209 0.13449911 0.93996987 5.3959400 0.203570833 0.08346667 0.228933333 0.14546667 2.761019e-01 9.96934e-07 FALSE TRUE
MSTRG.10801.6 ENSG00000138592 HEK293_OSMI2_2hA HEK293_TMG_2hB USP8 protein_coding   14.08589 1.201736 26.21299 0.3083042 2.647615 4.435685 0.050355441 0.13116519 0.0000000 0.06580569 0.00000000 -3.8193125 0.029437500 0.15366667 0.000000000 -0.15366667 5.934267e-03 9.96934e-07 FALSE TRUE
MSTRG.10801.9 ENSG00000138592 HEK293_OSMI2_2hA HEK293_TMG_2hB USP8 protein_coding   14.08589 1.201736 26.21299 0.3083042 2.647615 4.435685 0.005465755 0.04372604 0.0000000 0.04372604 0.00000000 -2.4256215 0.009141667 0.07313333 0.000000000 -0.07313333 2.530680e-01 9.96934e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000138592 E001 0.0000000       15 50424380 50424381 2 +      
ENSG00000138592 E002 0.0000000       15 50424382 50424386 5 +      
ENSG00000138592 E003 0.0000000       15 50424387 50424391 5 +      
ENSG00000138592 E004 0.7469680 0.0170608924 4.875333e-01 6.252021e-01 15 50424392 50424398 7 + 0.212 0.000 -9.961
ENSG00000138592 E005 1.0436561 0.0157804035 2.962321e-01 4.374315e-01 15 50424399 50424404 6 + 0.275 0.000 -11.969
ENSG00000138592 E006 4.0435055 0.0125606925 2.539356e-01 3.905640e-01 15 50424405 50424414 10 + 0.618 0.428 -0.906
ENSG00000138592 E007 7.6104298 0.0021938835 4.856857e-02 1.066246e-01 15 50424415 50424426 12 + 0.848 0.547 -1.261
ENSG00000138592 E008 11.0363340 0.0017093357 1.152984e-02 3.241803e-02 15 50424427 50424447 21 + 0.992 0.640 -1.390
ENSG00000138592 E009 26.5822037 0.0231211969 2.463342e-02 6.112523e-02 15 50424448 50424493 46 + 1.335 1.105 -0.814
ENSG00000138592 E010 28.5327622 0.0108016397 5.093193e-03 1.618536e-02 15 50424494 50424514 21 + 1.367 1.106 -0.923
ENSG00000138592 E011 23.7158371 0.0008516840 5.192813e-02 1.125973e-01 15 50424515 50424595 81 + 1.279 1.134 -0.514
ENSG00000138592 E012 24.3740469 0.0007733441 3.910245e-02 8.937709e-02 15 50424596 50424654 59 + 1.290 1.134 -0.553
ENSG00000138592 E013 0.0000000       15 50426894 50426920 27 +      
ENSG00000138592 E014 5.5409868 0.0383606463 5.275767e-01 6.606111e-01 15 50432338 50432499 162 + 0.712 0.639 -0.310
ENSG00000138592 E015 25.6568567 0.0008388321 1.996906e-03 7.216447e-03 15 50439009 50439025 17 + 1.323 1.045 -0.988
ENSG00000138592 E016 24.4558350 0.0007698080 4.108926e-03 1.344799e-02 15 50439026 50439042 17 + 1.301 1.045 -0.914
ENSG00000138592 E017 49.0289274 0.0004444019 5.489725e-03 1.726104e-02 15 50439043 50439177 135 + 1.580 1.432 -0.506
ENSG00000138592 E018 56.2670736 0.0026497179 1.713425e-02 4.523773e-02 15 50441349 50441493 145 + 1.633 1.518 -0.391
ENSG00000138592 E019 0.2214452 0.0391017797 6.353513e-02   15 50444571 50444616 46 + 0.000 0.266 12.568
ENSG00000138592 E020 46.2470331 0.0005006052 5.012542e-02 1.093883e-01 15 50449400 50449485 86 + 1.546 1.459 -0.298
ENSG00000138592 E021 0.0000000       15 50458503 50458583 81 +      
ENSG00000138592 E022 74.4060790 0.0010647933 9.853012e-04 3.908750e-03 15 50459000 50459162 163 + 1.754 1.608 -0.496
ENSG00000138592 E023 41.4136933 0.0008063018 1.972500e-04 9.520375e-04 15 50462280 50462322 43 + 1.517 1.251 -0.921
ENSG00000138592 E024 72.3328537 0.0003735777 5.259969e-05 2.947397e-04 15 50465047 50465191 145 + 1.747 1.550 -0.671
ENSG00000138592 E025 61.1886859 0.0004229646 3.951457e-04 1.755844e-03 15 50471633 50471795 163 + 1.678 1.495 -0.622
ENSG00000138592 E026 45.2692233 0.0039884647 6.479943e-02 1.347137e-01 15 50476849 50476993 145 + 1.538 1.446 -0.315
ENSG00000138592 E027 0.1451727 0.0428788683 1.000000e+00   15 50476994 50476997 4 + 0.051 0.000 -9.245
ENSG00000138592 E028 62.0547160 0.0003857507 1.609734e-01 2.763890e-01 15 50477276 50477499 224 + 1.662 1.634 -0.096
ENSG00000138592 E029 155.3778315 0.0002208016 5.929459e-04 2.507911e-03 15 50481481 50482065 585 + 2.063 1.986 -0.259
ENSG00000138592 E030 38.2612647 0.0005589237 2.015949e-01 3.281685e-01 15 50484275 50484361 87 + 1.462 1.419 -0.148
ENSG00000138592 E031 0.2944980 0.3807453525 1.000000e+00   15 50489494 50489800 307 + 0.097 0.000 -9.704
ENSG00000138592 E032 62.2235930 0.0003695079 9.007635e-01 9.407063e-01 15 50489801 50489881 81 + 1.653 1.704 0.175
ENSG00000138592 E033 102.0777881 0.0004076549 6.529108e-01 7.635261e-01 15 50490263 50490525 263 + 1.865 1.903 0.130
ENSG00000138592 E034 84.6310526 0.0025035391 9.181397e-01 9.522649e-01 15 50492701 50492913 213 + 1.781 1.835 0.182
ENSG00000138592 E035 5.4710450 0.0658264089 3.067337e-02 7.327934e-02 15 50493185 50494069 885 + 0.743 0.264 -2.436
ENSG00000138592 E036 79.3812483 0.0004342258 3.223544e-02 7.630581e-02 15 50494070 50494280 211 + 1.733 1.899 0.561
ENSG00000138592 E037 92.9330785 0.0004158721 1.080284e-02 3.069420e-02 15 50495848 50496084 237 + 1.798 1.975 0.595
ENSG00000138592 E038 66.8005652 0.0003532340 3.459582e-01 4.901012e-01 15 50497089 50497231 143 + 1.672 1.783 0.378
ENSG00000138592 E039 1.2532576 0.0837856880 3.058387e-02 7.311254e-02 15 50498497 50498595 99 + 0.177 0.638 2.729
ENSG00000138592 E040 56.8771883 0.0004214756 1.293376e-02 3.575668e-02 15 50498596 50498728 133 + 1.587 1.789 0.685
ENSG00000138592 E041 246.9306161 0.0106106319 1.931309e-15 7.033463e-14 15 50498903 50514421 15519 + 2.150 2.590 1.468