ENSG00000138459

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000460615 ENSG00000138459 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC35A5 protein_coding retained_intron 15.31739 2.869767 26.32289 0.5070823 2.334042 3.192842 4.8332654 0.00000000 8.601534 0.00000000 0.6960948 9.750126 0.22928333 0.0000000 0.32760000 0.32760000 3.715636e-25 3.715636e-25 FALSE FALSE
ENST00000468642 ENSG00000138459 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC35A5 protein_coding protein_coding 15.31739 2.869767 26.32289 0.5070823 2.334042 3.192842 0.8851416 0.00000000 1.771878 0.00000000 0.9966833 7.477255 0.03581667 0.0000000 0.06143333 0.06143333 4.789613e-01 3.715636e-25 FALSE FALSE
ENST00000492406 ENSG00000138459 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC35A5 protein_coding protein_coding 15.31739 2.869767 26.32289 0.5070823 2.334042 3.192842 7.2994484 2.64419054 11.291262 0.40137602 0.4624803 2.090140 0.61552083 0.9294333 0.43293333 -0.49650000 2.033000e-05 3.715636e-25 FALSE TRUE
MSTRG.23430.4 ENSG00000138459 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC35A5 protein_coding   15.31739 2.869767 26.32289 0.5070823 2.334042 3.192842 1.0577190 0.03574622 2.818059 0.03574622 0.3219971 5.950016 0.04555833 0.0149000 0.10953333 0.09463333 3.637233e-03 3.715636e-25 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000138459 E001 0.8900644 0.0594482662 3.291780e-01 4.726885e-01 3 112561709 112561822 114 + 0.273 0.000 -10.077
ENSG00000138459 E002 1.0340911 0.0121989077 8.892754e-01 9.332205e-01 3 112562037 112562044 8 + 0.273 0.237 -0.269
ENSG00000138459 E003 15.8509753 0.0014331683 3.486574e-01 4.928930e-01 3 112562045 112562071 27 + 1.145 1.048 -0.351
ENSG00000138459 E004 73.8030054 0.0003833130 2.749594e-02 6.699967e-02 3 112562072 112562239 168 + 1.794 1.683 -0.375
ENSG00000138459 E005 55.1533366 0.0004758724 1.671773e-01 2.845242e-01 3 112562240 112562273 34 + 1.665 1.588 -0.259
ENSG00000138459 E006 67.6869470 0.0003421587 1.116435e-03 4.358325e-03 3 112563385 112563460 76 + 1.770 1.588 -0.616
ENSG00000138459 E007 65.2919087 0.0003618984 1.360253e-02 3.731153e-02 3 112563461 112563533 73 + 1.747 1.612 -0.457
ENSG00000138459 E008 0.4439371 0.0215822517 8.800024e-01 9.270480e-01 3 112564128 112564366 239 + 0.158 0.000 -11.227
ENSG00000138459 E009 66.9588905 0.0004231043 4.757916e-03 1.525527e-02 3 112569171 112569269 99 + 1.760 1.604 -0.528
ENSG00000138459 E010 0.5985731 0.0203995849 6.782346e-01 7.827615e-01 3 112570439 112570539 101 + 0.158 0.237 0.730
ENSG00000138459 E011 91.0386806 0.0022174654 7.466883e-06 5.105307e-05 3 112570540 112570670 131 + 1.904 1.656 -0.840
ENSG00000138459 E012 81.2773454 0.0008188953 3.706056e-04 1.660678e-03 3 112573889 112573956 68 + 1.849 1.663 -0.628
ENSG00000138459 E013 133.8559088 0.0002608439 8.500591e-07 7.169771e-06 3 112580546 112580706 161 + 2.063 1.867 -0.660
ENSG00000138459 E014 211.4899938 0.0006507673 6.697874e-02 1.383501e-01 3 112580707 112581178 472 + 2.239 2.191 -0.159
ENSG00000138459 E015 66.5025670 0.0004673986 8.340129e-01 8.960542e-01 3 112581179 112581244 66 + 1.728 1.750 0.072
ENSG00000138459 E016 77.9937854 0.0003689877 7.710275e-01 8.521855e-01 3 112581245 112581326 82 + 1.797 1.822 0.083
ENSG00000138459 E017 66.6456394 0.0004402716 2.234206e-01 3.544714e-01 3 112582671 112582739 69 + 1.719 1.792 0.249
ENSG00000138459 E018 447.1744737 0.0005087440 2.621674e-32 5.179022e-30 3 112582740 112585579 2840 + 2.504 2.722 0.726
ENSG00000138459 E019 0.7385489 0.0466789931 4.250426e-01 5.679796e-01 3 112585581 112585740 160 + 0.238 0.000 -11.645