ENSG00000138433

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000342016 ENSG00000138433 HEK293_OSMI2_2hA HEK293_TMG_2hB CIR1 protein_coding protein_coding 11.97212 6.973531 17.19586 0.2972203 0.9083296 1.300871 10.9443931 5.8807809 16.5299907 0.04242325 1.0506221 1.4894276 0.90480833 0.8457333 0.9602 0.11446667 0.005032429 0.00111582 FALSE TRUE
ENST00000377973 ENSG00000138433 HEK293_OSMI2_2hA HEK293_TMG_2hB CIR1 protein_coding protein_coding 11.97212 6.973531 17.19586 0.2972203 0.9083296 1.300871 0.8165179 0.8892952 0.5846915 0.16235009 0.1182796 -0.5966532 0.07031667 0.1260667 0.0348 -0.09126667 0.001115820 0.00111582 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000138433 E001 273.7087841 0.0002745691 0.0002821073 0.00130706 2 174348022 174349134 1113 - 2.387 2.459 0.242
ENSG00000138433 E002 31.2593573 0.0014983156 0.0155422998 0.04171721 2 174349135 174349172 38 - 1.427 1.583 0.535
ENSG00000138433 E003 66.4936937 0.0003561938 0.2919415752 0.43287069 2 174350660 174350745 86 - 1.788 1.835 0.159
ENSG00000138433 E004 105.5454772 0.0009458548 0.1237797468 0.22534573 2 174351619 174351725 107 - 2.018 1.961 -0.192
ENSG00000138433 E005 1.0663419 0.4805482640 0.3618893157 0.50630563 2 174351726 174351963 238 - 0.210 0.417 1.378
ENSG00000138433 E006 6.2979089 0.0025877615 0.0002422474 0.00114267 2 174358173 174358536 364 - 0.635 1.088 1.759
ENSG00000138433 E007 0.5838113 0.3488547262 1.0000000000 1.00000000 2 174378687 174378922 236 - 0.209 0.176 -0.305
ENSG00000138433 E008 111.4510542 0.0011625489 0.1198062011 0.21970153 2 174378923 174379040 118 - 2.042 1.985 -0.192
ENSG00000138433 E009 71.3013294 0.0003741427 0.0647644256 0.13465869 2 174380202 174380250 49 - 1.858 1.776 -0.277
ENSG00000138433 E010 72.5531223 0.0004146698 0.6242912844 0.74065098 2 174380651 174380715 65 - 1.849 1.828 -0.070
ENSG00000138433 E011 48.1172667 0.0005758596 0.9365564807 0.96409822 2 174380804 174380814 11 - 1.667 1.672 0.016
ENSG00000138433 E012 74.0954813 0.0007143499 0.8182256690 0.88511114 2 174381704 174381777 74 - 1.854 1.844 -0.033
ENSG00000138433 E013 0.1515154 0.0433689585 1.0000000000   2 174387263 174387684 422 - 0.082 0.001 -6.959
ENSG00000138433 E014 73.2860424 0.0003425717 0.0681574198 0.14031055 2 174387685 174387779 95 - 1.870 1.791 -0.269
ENSG00000138433 E015 62.5661353 0.0004734297 0.0185758937 0.04839694 2 174395551 174395712 162 - 1.809 1.695 -0.387