ENSG00000138400

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000374412 ENSG00000138400 HEK293_OSMI2_2hA HEK293_TMG_2hB MDH1B protein_coding protein_coding 0.627017 0.6462523 0.7523499 0.1776226 0.02277398 0.2162028 0.04819335 0.01982473 0.13327597 0.01982473 0.10313412 2.2642156 0.08607500 0.01983333 0.16966667 0.14983333 7.068461e-01 4.902063e-06 FALSE TRUE
ENST00000432911 ENSG00000138400 HEK293_OSMI2_2hA HEK293_TMG_2hB MDH1B protein_coding nonsense_mediated_decay 0.627017 0.6462523 0.7523499 0.1776226 0.02277398 0.2162028 0.06615132 0.08354349 0.01589421 0.06082132 0.01589421 -1.8530076 0.11254583 0.15130000 0.02170000 -0.12960000 6.084320e-01 4.902063e-06 TRUE TRUE
ENST00000436472 ENSG00000138400 HEK293_OSMI2_2hA HEK293_TMG_2hB MDH1B protein_coding nonsense_mediated_decay 0.627017 0.6462523 0.7523499 0.1776226 0.02277398 0.2162028 0.04375336 0.00000000 0.09946380 0.00000000 0.09946380 3.4523819 0.05710417 0.00000000 0.13693333 0.13693333 8.483702e-01 4.902063e-06 TRUE TRUE
ENST00000449792 ENSG00000138400 HEK293_OSMI2_2hA HEK293_TMG_2hB MDH1B protein_coding protein_coding 0.627017 0.6462523 0.7523499 0.1776226 0.02277398 0.2162028 0.33831992 0.29818487 0.46034014 0.10556143 0.05505926 0.6099084 0.51654167 0.44716667 0.61253333 0.16536667 4.489834e-01 4.902063e-06 FALSE TRUE
ENST00000454776 ENSG00000138400 HEK293_OSMI2_2hA HEK293_TMG_2hB MDH1B protein_coding protein_coding 0.627017 0.6462523 0.7523499 0.1776226 0.02277398 0.2162028 0.04662426 0.00000000 0.04337578 0.00000000 0.04337578 2.4161852 0.10491667 0.00000000 0.05916667 0.05916667 8.200224e-01 4.902063e-06 FALSE TRUE
ENST00000471988 ENSG00000138400 HEK293_OSMI2_2hA HEK293_TMG_2hB MDH1B protein_coding retained_intron 0.627017 0.6462523 0.7523499 0.1776226 0.02277398 0.2162028 0.08397476 0.24469916 0.00000000 0.07509587 0.00000000 -4.6707223 0.12282500 0.38170000 0.00000000 -0.38170000 4.902063e-06 4.902063e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000138400 E001 0.0000000       2 206737763 206737827 65 -      
ENSG00000138400 E002 1.9134275 0.062554467 0.258388982 0.39563092 2 206737828 206738224 397 - 0.333 0.554 1.164
ENSG00000138400 E003 0.6653823 0.018127780 0.411816821 0.55537138 2 206738225 206738256 32 - 0.271 0.136 -1.237
ENSG00000138400 E004 3.4364211 0.004843932 0.367542068 0.51191941 2 206738257 206738511 255 - 0.703 0.595 -0.462
ENSG00000138400 E005 2.2936904 0.008464559 0.956279023 0.97661584 2 206739593 206739658 66 - 0.520 0.552 0.150
ENSG00000138400 E006 2.0002245 0.008273504 0.987179424 0.99604088 2 206739659 206739661 3 - 0.480 0.505 0.121
ENSG00000138400 E007 2.5819474 0.006263924 0.164869789 0.28153274 2 206741054 206741104 51 - 0.650 0.452 -0.919
ENSG00000138400 E008 0.8126288 0.232105336 0.046557206 0.10304484 2 206741105 206741382 278 - 0.000 0.400 11.894
ENSG00000138400 E009 2.4219984 0.088107868 0.768483312 0.85039298 2 206745622 206745673 52 - 0.555 0.506 -0.230
ENSG00000138400 E010 2.4638287 0.010392735 0.670330641 0.77681004 2 206746287 206746426 140 - 0.556 0.506 -0.237
ENSG00000138400 E011 2.0554928 0.011910416 0.736268896 0.82680174 2 206749020 206749183 164 - 0.436 0.506 0.349
ENSG00000138400 E012 0.7718584 0.016791263 0.806907382 0.87734089 2 206750934 206751075 142 - 0.271 0.239 -0.240
ENSG00000138400 E013 9.1996515 0.003464927 0.590621329 0.71345686 2 206755009 206755505 497 - 0.985 1.065 0.293
ENSG00000138400 E014 1.2177675 0.011538270 0.001795621 0.00658590 2 206756619 206756897 279 - 0.000 0.552 12.658
ENSG00000138400 E015 2.6570977 0.068451886 0.203914906 0.33108843 2 206756898 206756911 14 - 0.437 0.668 1.078
ENSG00000138400 E016 6.5456558 0.003038917 0.027487083 0.06698067 2 206756912 206757040 129 - 0.727 1.007 1.081
ENSG00000138400 E017 7.6110738 0.002706566 0.456617850 0.59700947 2 206757237 206757371 135 - 0.972 0.920 -0.192
ENSG00000138400 E018 3.3819065 0.005117639 0.073913369 0.14976754 2 206760901 206761013 113 - 0.750 0.505 -1.070
ENSG00000138400 E019 5.4134538 0.003406866 0.032993092 0.07779020 2 206765250 206765328 79 - 0.915 0.669 -0.976