ENSG00000138336

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000373644 ENSG00000138336 HEK293_OSMI2_2hA HEK293_TMG_2hB TET1 protein_coding protein_coding 2.071364 1.700996 2.011864 0.1544228 0.0616189 0.2408501 1.0265519 0.3664519 1.2880468 0.1005087 0.07275374 1.785805 0.4735708 0.2178667 0.6416 0.4237333 0.0003144568 0.0003144568 FALSE TRUE
MSTRG.4071.2 ENSG00000138336 HEK293_OSMI2_2hA HEK293_TMG_2hB TET1 protein_coding   2.071364 1.700996 2.011864 0.1544228 0.0616189 0.2408501 0.5535037 0.1982491 0.5724767 0.1013113 0.05094760 1.483890 0.2503083 0.1074667 0.2836 0.1761333 0.2114662230 0.0003144568 FALSE TRUE
MSTRG.4071.3 ENSG00000138336 HEK293_OSMI2_2hA HEK293_TMG_2hB TET1 protein_coding   2.071364 1.700996 2.011864 0.1544228 0.0616189 0.2408501 0.4913080 1.1362946 0.1513407 0.1387719 0.09351562 -2.828795 0.2761083 0.6746667 0.0748 -0.5998667 0.0016085210 0.0003144568 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000138336 E001 7.2894385 0.0232001647 1.123906e-03 4.383428e-03 10 68560337 68560742 406 + 1.009 0.633 -1.481
ENSG00000138336 E002 48.9453653 0.0011719254 9.900101e-12 2.063759e-10 10 68572217 68574252 2036 + 1.757 1.507 -0.853
ENSG00000138336 E003 6.3493367 0.1013918599 3.020482e-01 4.438817e-01 10 68600981 68601034 54 + 0.900 0.757 -0.556
ENSG00000138336 E004 72.9684939 0.0122623394 9.219239e-06 6.178129e-05 10 68644698 68647005 2308 + 1.899 1.751 -0.498
ENSG00000138336 E005 0.1515154 0.0429966588 7.420794e-01   10 68648237 68648337 101 + 0.088 0.000 -8.391
ENSG00000138336 E006 8.0866627 0.0020970962 1.768979e-01 2.972204e-01 10 68651846 68651936 91 + 0.959 0.907 -0.195
ENSG00000138336 E007 9.8060817 0.0023027285 1.213794e-01 2.218926e-01 10 68652501 68652594 94 + 1.037 0.977 -0.221
ENSG00000138336 E008 16.3427549 0.0038299743 2.707565e-01 4.094088e-01 10 68667045 68667256 212 + 1.218 1.231 0.048
ENSG00000138336 E009 14.0615134 0.0167108651 2.965462e-02 7.128697e-02 10 68672895 68673045 151 + 1.205 1.074 -0.470
ENSG00000138336 E010 5.7357018 0.0075492185 1.658676e-02 4.403093e-02 10 68673401 68673487 87 + 0.876 0.634 -0.979
ENSG00000138336 E011 10.3790199 0.0018983750 2.990083e-02 7.176586e-02 10 68681399 68681488 90 + 1.080 0.955 -0.459
ENSG00000138336 E012 12.4287537 0.0016092438 3.035274e-03 1.036975e-02 10 68682836 68682973 138 + 1.168 0.977 -0.692
ENSG00000138336 E013 23.5116194 0.0008263661 1.211350e-01 2.215591e-01 10 68686356 68686707 352 + 1.371 1.373 0.009
ENSG00000138336 E014 197.1594602 0.0113127519 1.064973e-18 5.866705e-17 10 68690808 68694487 3680 + 2.076 2.496 1.402