ENSG00000138175

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000260746 ENSG00000138175 HEK293_OSMI2_2hA HEK293_TMG_2hB ARL3 protein_coding protein_coding 32.54636 35.00617 36.77872 5.568204 0.3253165 0.07124238 1.718906 1.689005 2.127328 0.3305967 0.08597906 0.3311180 0.05752917 0.04773333 0.05786667 0.01013333 0.539107542 2.676635e-06 FALSE TRUE
MSTRG.4558.1 ENSG00000138175 HEK293_OSMI2_2hA HEK293_TMG_2hB ARL3 protein_coding   32.54636 35.00617 36.77872 5.568204 0.3253165 0.07124238 3.960202 4.020301 5.616150 0.1916231 0.71038177 0.4812605 0.11606250 0.11930000 0.15280000 0.03350000 0.593891068 2.676635e-06 FALSE TRUE
MSTRG.4558.5 ENSG00000138175 HEK293_OSMI2_2hA HEK293_TMG_2hB ARL3 protein_coding   32.54636 35.00617 36.77872 5.568204 0.3253165 0.07124238 20.361412 25.317632 20.377903 3.8740712 0.33553622 -0.3129988 0.62673750 0.72550000 0.55420000 -0.17130000 0.002646496 2.676635e-06 FALSE TRUE
MSTRG.4558.7 ENSG00000138175 HEK293_OSMI2_2hA HEK293_TMG_2hB ARL3 protein_coding   32.54636 35.00617 36.77872 5.568204 0.3253165 0.07124238 5.313065 2.702668 7.107562 1.4670951 0.81006787 1.3916706 0.16185417 0.07060000 0.19296667 0.12236667 0.528298875 2.676635e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000138175 E001 3.2679603 0.0702124833 1.399174e-02 0.0382076268 10 102665306 102665458 153 - 0.307 0.756 2.195
ENSG00000138175 E002 2.1995301 0.1537765933 5.258292e-02 0.1137580213 10 102665459 102665507 49 - 0.226 0.619 2.210
ENSG00000138175 E003 141.8543128 0.0002788533 2.049303e-02 0.0525312613 10 102673731 102676645 2915 - 2.195 2.131 -0.212
ENSG00000138175 E004 5.6268813 0.0109099797 4.408269e-01 0.5824818158 10 102676646 102676662 17 - 0.876 0.774 -0.398
ENSG00000138175 E005 9.8598305 0.0026867689 2.694338e-01 0.4079460153 10 102676663 102676687 25 - 0.965 1.084 0.436
ENSG00000138175 E006 235.4884109 0.0002249627 2.373723e-04 0.0011223850 10 102676688 102676941 254 - 2.327 2.410 0.277
ENSG00000138175 E007 1.1479395 0.0119010187 8.170842e-01 0.8843156672 10 102685347 102685386 40 - 0.307 0.350 0.266
ENSG00000138175 E008 331.1008791 0.0007018681 4.064722e-03 0.0133231147 10 102685816 102686001 186 - 2.490 2.547 0.190
ENSG00000138175 E009 211.1302783 0.0002859947 5.327171e-03 0.0168219568 10 102689893 102689943 51 - 2.292 2.361 0.229
ENSG00000138175 E010 284.3972326 0.0001982214 2.073233e-01 0.3352939142 10 102699373 102699489 117 - 2.472 2.450 -0.073
ENSG00000138175 E011 238.5755064 0.0002325312 2.095921e-05 0.0001293998 10 102705346 102705489 144 - 2.431 2.341 -0.300
ENSG00000138175 E012 1.0267017 0.4113684876 9.280193e-01 0.9586803529 10 102713795 102713842 48 - 0.306 0.291 -0.102
ENSG00000138175 E013 0.7406253 0.0155918816 1.315171e-02 0.0362592124 10 102714148 102714272 125 - 0.433 0.000 -11.442
ENSG00000138175 E014 86.1429817 0.0003260817 3.509716e-06 0.0000258800 10 102714273 102714397 125 - 2.032 1.863 -0.566