ENSG00000138085

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000380171 ENSG00000138085 HEK293_OSMI2_2hA HEK293_TMG_2hB ATRAID protein_coding protein_coding 148.2814 236.5249 118.6278 9.651528 0.2539722 -0.9954889 79.39100 123.3808 66.83686 5.875021 1.128014 -0.8843032 0.5372667 0.5214667 0.5634667 0.0420 0.174613931 0.0007431552 FALSE  
ENST00000405489 ENSG00000138085 HEK293_OSMI2_2hA HEK293_TMG_2hB ATRAID protein_coding protein_coding 148.2814 236.5249 118.6278 9.651528 0.2539722 -0.9954889 65.39961 110.8293 48.89290 4.227240 0.731360 -1.1804771 0.4356042 0.4687333 0.4121333 -0.0566 0.007435833 0.0007431552 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000138085 E001 250.139964 0.0096903619 2.084448e-01 0.3366057481 2 27212041 27212176 136 + 2.392 2.325 -0.221
ENSG00000138085 E002 620.916718 0.0040263812 8.413447e-01 0.9010404996 2 27212177 27212369 193 + 2.742 2.736 -0.017
ENSG00000138085 E003 948.775052 0.0022664034 9.351465e-01 0.9631638702 2 27212370 27212467 98 + 2.920 2.923 0.011
ENSG00000138085 E004 373.803205 0.0056008163 3.347194e-01 0.4784516188 2 27212468 27212607 140 + 2.487 2.528 0.138
ENSG00000138085 E005 19.833736 0.0082145341 2.310000e-03 0.0081823092 2 27212866 27213176 311 + 1.443 1.175 -0.938
ENSG00000138085 E006 742.114637 0.0015912454 7.137481e-01 0.8101429091 2 27213177 27213194 18 + 2.808 2.818 0.032
ENSG00000138085 E007 1408.565472 0.0009256098 7.162625e-01 0.8119687474 2 27213195 27213298 104 + 3.097 3.092 -0.016
ENSG00000138085 E008 6.122430 0.0228021531 7.980159e-01 0.8711103967 2 27213299 27213433 135 + 0.775 0.817 0.166
ENSG00000138085 E009 1356.717529 0.0004429001 1.216795e-02 0.0339321577 2 27215321 27215392 72 + 3.102 3.070 -0.105
ENSG00000138085 E010 1.027647 0.3165122865 2.374888e-01 0.3712486110 2 27215393 27215414 22 + 0.473 0.208 -1.682
ENSG00000138085 E011 1570.702618 0.0008443023 1.513556e-03 0.0056799034 2 27215474 27215545 72 + 3.176 3.129 -0.156
ENSG00000138085 E012 1861.739741 0.0007071649 6.874311e-01 0.7897811549 2 27215632 27215753 122 + 3.219 3.215 -0.011
ENSG00000138085 E013 4.495197 0.1495353267 6.421527e-01 0.7550093904 2 27216374 27216522 149 + 0.774 0.675 -0.402
ENSG00000138085 E014 1399.956233 0.0005129666 4.969546e-05 0.0002802565 2 27216523 27216620 98 + 3.047 3.108 0.202
ENSG00000138085 E015 1826.760060 0.0017337467 4.650575e-02 0.1029514072 2 27216844 27217178 335 + 3.176 3.218 0.140