ENSG00000138071

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000260641 ENSG00000138071 HEK293_OSMI2_2hA HEK293_TMG_2hB ACTR2 protein_coding protein_coding 84.72864 33.75967 142.9642 3.484047 5.485182 2.081955 63.96924 29.793462 105.74343 3.4254469 5.402057 1.827153 0.7972667 0.8806333 0.7390000 -0.1416333 1.536030e-06 1.099309e-06 FALSE TRUE
MSTRG.18542.1 ENSG00000138071 HEK293_OSMI2_2hA HEK293_TMG_2hB ACTR2 protein_coding   84.72864 33.75967 142.9642 3.484047 5.485182 2.081955 20.65959 3.966211 37.01518 0.2606199 1.487133 3.219041 0.2011167 0.1193667 0.2594667 0.1401000 1.099309e-06 1.099309e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000138071 E001 0.2966881 0.0269255783 1.000000e+00   2 65227700 65227702 3 + 0.109 0.000 -9.319
ENSG00000138071 E002 3.2760273 0.0264730858 4.429271e-03 1.434282e-02 2 65227703 65227787 85 + 0.616 0.000 -12.773
ENSG00000138071 E003 83.7533158 0.0051453077 3.515856e-04 1.586482e-03 2 65227788 65227830 43 + 1.840 1.732 -0.363
ENSG00000138071 E004 83.3772321 0.0051474293 6.180038e-04 2.599617e-03 2 65227831 65227832 2 + 1.836 1.738 -0.331
ENSG00000138071 E005 83.6675775 0.0048966854 4.927034e-04 2.132343e-03 2 65227833 65227836 4 + 1.838 1.738 -0.337
ENSG00000138071 E006 461.7433393 0.0007559047 5.535305e-23 4.902510e-21 2 65227837 65227957 121 + 2.581 2.444 -0.455
ENSG00000138071 E007 678.9721053 0.0020874854 2.587786e-18 1.355400e-16 2 65239852 65239962 111 + 2.747 2.613 -0.447
ENSG00000138071 E008 0.5975289 0.0373191572 4.229868e-01 5.661110e-01 2 65241972 65242048 77 + 0.195 0.000 -10.312
ENSG00000138071 E009 0.1515154 0.0438308011 1.000000e+00   2 65242049 65242063 15 + 0.058 0.000 -8.312
ENSG00000138071 E010 0.4418608 0.0247446323 6.223123e-01 7.391363e-01 2 65246185 65246307 123 + 0.154 0.000 -9.897
ENSG00000138071 E011 950.6511467 0.0001234918 8.116232e-42 3.170301e-39 2 65246524 65246739 216 + 2.887 2.798 -0.295
ENSG00000138071 E012 479.5433285 0.0012076353 1.267093e-07 1.262010e-06 2 65251027 65251099 73 + 2.579 2.558 -0.071
ENSG00000138071 E013 640.0197314 0.0001619709 1.247721e-08 1.511651e-07 2 65253728 65253864 137 + 2.697 2.716 0.063
ENSG00000138071 E014 624.0986684 0.0001311382 8.625003e-01 9.153288e-01 2 65255545 65255694 150 + 2.664 2.783 0.396
ENSG00000138071 E015 839.8696098 0.0013919237 3.866070e-10 6.182285e-09 2 65261247 65261392 146 + 2.822 2.795 -0.093
ENSG00000138071 E016 861.3831772 0.0001211166 4.047757e-10 6.449957e-09 2 65265043 65265175 133 + 2.824 2.849 0.085
ENSG00000138071 E017 5408.8641797 0.0033167758 8.021772e-20 4.989168e-18 2 65268564 65271253 2690 + 3.567 3.815 0.824