ENSG00000138035

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000415374 ENSG00000138035 HEK293_OSMI2_2hA HEK293_TMG_2hB PNPT1 protein_coding nonsense_mediated_decay 25.83479 6.344485 43.32309 0.3990589 0.4740363 2.769622 3.1414867 0.5368909 3.4328830 0.27138059 0.36013790 2.6542923 0.11638333 0.08916667 0.07920000 -0.009966667 0.9284182439 3.744739e-05 TRUE TRUE
ENST00000415489 ENSG00000138035 HEK293_OSMI2_2hA HEK293_TMG_2hB PNPT1 protein_coding nonsense_mediated_decay 25.83479 6.344485 43.32309 0.3990589 0.4740363 2.769622 0.3755677 0.3575828 0.1878753 0.35758278 0.09934134 -0.8934778 0.01888333 0.05586667 0.00430000 -0.051566667 1.0000000000 3.744739e-05 TRUE FALSE
ENST00000447944 ENSG00000138035 HEK293_OSMI2_2hA HEK293_TMG_2hB PNPT1 protein_coding protein_coding 25.83479 6.344485 43.32309 0.3990589 0.4740363 2.769622 5.6519403 2.7028094 11.2208854 0.19141354 0.38549189 2.0496121 0.30040417 0.43150000 0.25913333 -0.172366667 0.0200249555 3.744739e-05 FALSE TRUE
ENST00000481066 ENSG00000138035 HEK293_OSMI2_2hA HEK293_TMG_2hB PNPT1 protein_coding retained_intron 25.83479 6.344485 43.32309 0.3990589 0.4740363 2.769622 1.1620254 0.6046909 1.2286803 0.08529515 0.15263016 1.0108707 0.07521667 0.09776667 0.02843333 -0.069333333 0.0006582296 3.744739e-05   FALSE
MSTRG.18434.4 ENSG00000138035 HEK293_OSMI2_2hA HEK293_TMG_2hB PNPT1 protein_coding   25.83479 6.344485 43.32309 0.3990589 0.4740363 2.769622 2.1628373 0.6198610 3.5871783 0.61986104 0.38471088 2.5137602 0.08490417 0.08846667 0.08270000 -0.005766667 0.4034956076 3.744739e-05 FALSE TRUE
MSTRG.18434.5 ENSG00000138035 HEK293_OSMI2_2hA HEK293_TMG_2hB PNPT1 protein_coding   25.83479 6.344485 43.32309 0.3990589 0.4740363 2.769622 10.7150594 1.3631212 19.9053669 0.31842493 0.99000107 3.8583511 0.33897917 0.21020000 0.46006667 0.249866667 0.0020684447 3.744739e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000138035 E001 85.4567913 0.0003938326 6.794322e-08 7.130707e-07 2 55634061 55634568 508 - 1.765 2.015 0.843
ENSG00000138035 E002 73.4036166 0.0025648736 7.749528e-05 4.162059e-04 2 55634569 55634849 281 - 1.707 1.925 0.736
ENSG00000138035 E003 63.1773839 0.0004215278 2.257438e-06 1.735864e-05 2 55634850 55635093 244 - 1.634 1.888 0.856
ENSG00000138035 E004 34.5826477 0.0053838658 1.983863e-01 3.241957e-01 2 55635094 55635141 48 - 1.416 1.526 0.379
ENSG00000138035 E005 42.5937771 0.0005905499 8.369364e-01 8.980187e-01 2 55635142 55635216 75 - 1.527 1.517 -0.037
ENSG00000138035 E006 104.8838701 0.0003032456 1.383714e-01 2.458282e-01 2 55635217 55635561 345 - 1.918 1.851 -0.224
ENSG00000138035 E007 36.2368739 0.0018942977 2.065285e-01 3.343748e-01 2 55635562 55635578 17 - 1.471 1.372 -0.344
ENSG00000138035 E008 220.1261357 0.0003696134 6.161475e-07 5.354463e-06 2 55635579 55636311 733 - 2.193 2.349 0.520
ENSG00000138035 E009 163.6787892 0.0235188924 1.894430e-01 3.130400e-01 2 55636312 55636369 58 - 2.071 2.207 0.455
ENSG00000138035 E010 125.3567901 0.0071133735 1.273637e-01 2.304685e-01 2 55636370 55636392 23 - 1.964 2.062 0.332
ENSG00000138035 E011 152.0269594 0.0002846099 3.996444e-03 1.313386e-02 2 55637552 55637599 48 - 2.044 2.154 0.368
ENSG00000138035 E012 174.1758773 0.0105905802 5.356060e-02 1.154842e-01 2 55640627 55640705 79 - 2.098 2.230 0.441
ENSG00000138035 E013 155.7349381 0.0011681006 7.297642e-05 3.944862e-04 2 55643158 55643213 56 - 2.045 2.201 0.523
ENSG00000138035 E014 184.9664268 0.0002207345 3.613069e-05 2.104748e-04 2 55643319 55643412 94 - 2.123 2.262 0.465
ENSG00000138035 E015 104.9701007 0.0002894374 5.760048e-04 2.444443e-03 2 55643413 55643425 13 - 1.876 2.028 0.511
ENSG00000138035 E016 169.2399231 0.0002936526 8.405442e-03 2.480158e-02 2 55644637 55644720 84 - 2.094 2.190 0.322
ENSG00000138035 E017 10.3318225 0.0091258558 5.360178e-01 6.678041e-01 2 55644721 55645348 628 - 0.929 1.014 0.317
ENSG00000138035 E018 198.5682486 0.0002481008 9.299975e-01 9.599440e-01 2 55645349 55645432 84 - 2.179 2.186 0.024
ENSG00000138035 E019 170.4065965 0.0017280283 1.661293e-01 2.831715e-01 2 55646259 55646322 64 - 2.122 2.069 -0.179
ENSG00000138035 E020 185.5082272 0.0006715142 2.847517e-02 6.896443e-02 2 55646415 55646486 72 - 2.164 2.085 -0.264
ENSG00000138035 E021 177.2849891 0.0002127536 8.032851e-01 8.747609e-01 2 55647347 55647400 54 - 2.132 2.127 -0.016
ENSG00000138035 E022 166.5905262 0.0018978922 6.748615e-01 7.803041e-01 2 55647401 55647453 53 - 2.100 2.123 0.074
ENSG00000138035 E023 0.1451727 0.0426874245 1.000000e+00   2 55654453 55654500 48 - 0.055 0.000 -7.797
ENSG00000138035 E024 150.4520120 0.0022236212 3.875378e-01 5.316801e-01 2 55654900 55654953 54 - 2.054 2.096 0.140
ENSG00000138035 E025 147.1716368 0.0002568845 6.183551e-01 7.360144e-01 2 55656131 55656220 90 - 2.047 2.071 0.079
ENSG00000138035 E026 121.3300866 0.0016285290 3.126373e-01 4.551673e-01 2 55656305 55656371 67 - 1.976 1.933 -0.146
ENSG00000138035 E027 116.1912910 0.0002936746 2.385622e-01 3.724650e-01 2 55660157 55660193 37 - 1.958 1.909 -0.164
ENSG00000138035 E028 121.0312185 0.0022518472 5.464167e-01 6.766083e-01 2 55661956 55662026 71 - 1.960 1.993 0.111
ENSG00000138035 E029 175.2142179 0.0081969017 3.636688e-01 5.080898e-01 2 55666991 55667093 103 - 2.133 2.087 -0.157
ENSG00000138035 E030 153.1410796 0.0093351561 1.153836e-01 2.134095e-01 2 55667862 55667958 97 - 2.082 1.991 -0.307
ENSG00000138035 E031 126.6105208 0.0002488645 1.779468e-01 2.985616e-01 2 55671319 55671376 58 - 1.995 1.941 -0.182
ENSG00000138035 E032 120.1872693 0.0003590979 4.706891e-02 1.039479e-01 2 55671995 55672046 52 - 1.978 1.892 -0.290
ENSG00000138035 E033 196.7550415 0.0036160006 6.510198e-03 1.994820e-02 2 55672893 55673079 187 - 2.195 2.072 -0.411
ENSG00000138035 E034 152.7467338 0.0013434761 2.851488e-05 1.705163e-04 2 55679682 55679795 114 - 2.093 1.906 -0.629
ENSG00000138035 E035 110.0560836 0.0002899840 1.209421e-05 7.883061e-05 2 55680712 55680759 48 - 1.956 1.738 -0.735
ENSG00000138035 E036 0.5911862 0.0184964063 6.365345e-01 7.505324e-01 2 55680760 55680854 95 - 0.189 0.000 -9.797
ENSG00000138035 E037 105.4633449 0.0006704993 1.091060e-03 4.273624e-03 2 55680855 55680918 64 - 1.931 1.768 -0.550
ENSG00000138035 E038 90.4307003 0.0003209951 1.677252e-02 4.443565e-02 2 55683785 55683834 50 - 1.860 1.738 -0.414
ENSG00000138035 E039 77.4958993 0.0003801837 5.144309e-02 1.117169e-01 2 55684943 55684993 51 - 1.792 1.685 -0.360
ENSG00000138035 E040 80.8959075 0.0003208011 1.397215e-03 5.297672e-03 2 55684994 55685048 55 - 1.821 1.641 -0.609
ENSG00000138035 E041 83.2852070 0.0006093676 8.470672e-07 7.146486e-06 2 55686370 55686440 71 - 1.845 1.547 -1.012
ENSG00000138035 E042 47.4632855 0.0004639076 6.208202e-04 2.609377e-03 2 55686441 55686444 4 - 1.605 1.342 -0.902
ENSG00000138035 E043 80.6380516 0.0003067699 1.105802e-06 9.094850e-06 2 55687645 55687705 61 - 1.833 1.537 -1.003
ENSG00000138035 E044 122.8874300 0.0010495615 1.926516e-09 2.707111e-08 2 55693663 55693863 201 - 2.013 1.699 -1.056