ENSG00000137872

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000316364 ENSG00000137872 HEK293_OSMI2_2hA HEK293_TMG_2hB SEMA6D protein_coding protein_coding 0.7400886 0.4041592 0.7274321 0.06773455 0.01473629 0.8323248 0.051064401 0.00000000 0.08227445 0.00000000 0.08227445 3.205931 0.07300000 0.0000000 0.11033333 0.11033333 0.9072259684 0.0002532172 FALSE TRUE
ENST00000354744 ENSG00000137872 HEK293_OSMI2_2hA HEK293_TMG_2hB SEMA6D protein_coding protein_coding 0.7400886 0.4041592 0.7274321 0.06773455 0.01473629 0.8323248 0.007393222 0.00000000 0.05914578 0.00000000 0.05914578 2.789641 0.01001250 0.0000000 0.08010000 0.08010000 0.9000971604 0.0002532172 FALSE TRUE
ENST00000355997 ENSG00000137872 HEK293_OSMI2_2hA HEK293_TMG_2hB SEMA6D protein_coding protein_coding 0.7400886 0.4041592 0.7274321 0.06773455 0.01473629 0.8323248 0.014845635 0.00000000 0.11876508 0.00000000 0.11876508 3.686669 0.02126250 0.0000000 0.17010000 0.17010000 0.9223630156 0.0002532172 FALSE TRUE
ENST00000389425 ENSG00000137872 HEK293_OSMI2_2hA HEK293_TMG_2hB SEMA6D protein_coding protein_coding 0.7400886 0.4041592 0.7274321 0.06773455 0.01473629 0.8323248 0.031845909 0.00000000 0.04517603 0.00000000 0.04517603 2.464042 0.02873333 0.0000000 0.06116667 0.06116667 0.8979569500 0.0002532172 FALSE TRUE
ENST00000536845 ENSG00000137872 HEK293_OSMI2_2hA HEK293_TMG_2hB SEMA6D protein_coding protein_coding 0.7400886 0.4041592 0.7274321 0.06773455 0.01473629 0.8323248 0.266909981 0.06119085 0.33329066 0.03166693 0.03830897 2.269667 0.31450000 0.1401333 0.45653333 0.31640000 0.0566915374 0.0002532172 FALSE TRUE
ENST00000558014 ENSG00000137872 HEK293_OSMI2_2hA HEK293_TMG_2hB SEMA6D protein_coding protein_coding 0.7400886 0.4041592 0.7274321 0.06773455 0.01473629 0.8323248 0.040603081 0.00000000 0.04015663 0.00000000 0.02739346 2.326440 0.05026250 0.0000000 0.05426667 0.05426667 0.6370039759 0.0002532172 FALSE TRUE
ENST00000558816 ENSG00000137872 HEK293_OSMI2_2hA HEK293_TMG_2hB SEMA6D protein_coding protein_coding 0.7400886 0.4041592 0.7274321 0.06773455 0.01473629 0.8323248 0.039277811 0.06001982 0.02367100 0.06001982 0.02367100 -1.056257 0.06952083 0.1837667 0.03173333 -0.15203333 0.8871387051 0.0002532172 FALSE TRUE
ENST00000560006 ENSG00000137872 HEK293_OSMI2_2hA HEK293_TMG_2hB SEMA6D protein_coding protein_coding 0.7400886 0.4041592 0.7274321 0.06773455 0.01473629 0.8323248 0.154909708 0.19987821 0.00000000 0.04980959 0.00000000 -4.391480 0.28064583 0.5046000 0.00000000 -0.50460000 0.0002532172 0.0002532172 FALSE TRUE
ENST00000560636 ENSG00000137872 HEK293_OSMI2_2hA HEK293_TMG_2hB SEMA6D protein_coding protein_coding 0.7400886 0.4041592 0.7274321 0.06773455 0.01473629 0.8323248 0.014178260 0.06861594 0.00000000 0.06861594 0.00000000 -2.974822 0.02127083 0.1272667 0.00000000 -0.12726667 0.6001020929 0.0002532172   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000137872 E001 0.1515154 0.042978140 1.000000e+00   15 47184101 47184418 318 + 0.077 0.000 -10.297
ENSG00000137872 E002 0.0000000       15 47185663 47185772 110 +      
ENSG00000137872 E003 0.1482932 0.041981449 1.704763e-01   15 47241240 47241370 131 + 0.000 0.190 13.282
ENSG00000137872 E004 0.0000000       15 47339067 47339219 153 +      
ENSG00000137872 E005 0.4428904 0.549293338 1.058123e-01 1.993281e-01 15 47412393 47412472 80 + 0.000 0.336 13.754
ENSG00000137872 E006 0.5169874 0.022062482 1.493224e-01 2.607419e-01 15 47470474 47470545 72 + 0.076 0.322 2.514
ENSG00000137872 E007 0.2955422 0.028978523 4.573391e-01   15 47600865 47600896 32 + 0.076 0.190 1.511
ENSG00000137872 E008 0.1482932 0.041981449 1.704763e-01   15 47603375 47603458 84 + 0.000 0.190 13.282
ENSG00000137872 E009 0.0000000       15 47716998 47717087 90 +      
ENSG00000137872 E010 0.0000000       15 47717402 47717456 55 +      
ENSG00000137872 E011 0.0000000       15 47717457 47717504 48 +      
ENSG00000137872 E012 1.1383625 0.013100080 3.559168e-01 5.002846e-01 15 47717505 47717692 188 + 0.248 0.423 1.099
ENSG00000137872 E013 0.1472490 0.042976382 1.000000e+00   15 47718461 47718488 28 + 0.076 0.000 -12.469
ENSG00000137872 E014 0.8793415 0.127465992 1.444304e-01 2.540519e-01 15 47718489 47718873 385 + 0.333 0.000 -14.327
ENSG00000137872 E015 0.0000000       15 47759723 47759744 22 +      
ENSG00000137872 E016 3.4343207 0.004918182 3.528327e-01 4.970855e-01 15 47759745 47759907 163 + 0.650 0.505 -0.656
ENSG00000137872 E017 0.0000000       15 47759908 47759983 76 +      
ENSG00000137872 E018 3.3184066 0.005117639 4.176736e-01 5.609974e-01 15 47760304 47760415 112 + 0.631 0.505 -0.573
ENSG00000137872 E019 2.3563361 0.006451638 5.527055e-01 6.819484e-01 15 47760978 47761038 61 + 0.520 0.423 -0.486
ENSG00000137872 E020 1.9446537 0.083119393 1.215062e-01 2.220817e-01 15 47761158 47761220 63 + 0.519 0.190 -2.068
ENSG00000137872 E021 2.1672699 0.034100192 6.606681e-01 7.692879e-01 15 47761330 47761431 102 + 0.494 0.424 -0.359
ENSG00000137872 E022 1.5458948 0.056917512 6.392761e-01 7.526982e-01 15 47761661 47761751 91 + 0.405 0.322 -0.484
ENSG00000137872 E023 2.3208617 0.007747326 5.517348e-01 6.811544e-01 15 47762200 47762319 120 + 0.520 0.423 -0.486
ENSG00000137872 E024 2.4119992 0.006874558 3.542696e-02 8.247354e-02 15 47763016 47763104 89 + 0.590 0.190 -2.394
ENSG00000137872 E025 3.5191286 0.004735265 2.995537e-02 7.187127e-02 15 47763850 47764067 218 + 0.703 0.322 -1.879
ENSG00000137872 E026 3.4415965 0.048039428 5.176366e-02 1.122985e-01 15 47764174 47764305 132 + 0.703 0.322 -1.883
ENSG00000137872 E027 3.8803342 0.004486201 2.056419e-01 3.332384e-01 15 47764638 47764793 156 + 0.703 0.505 -0.878
ENSG00000137872 E028 4.1866124 0.004669011 3.220050e-01 4.652730e-01 15 47764883 47765056 174 + 0.719 0.574 -0.624
ENSG00000137872 E029 0.4502799 0.025351670 3.906878e-01 5.346449e-01 15 47765057 47765480 424 + 0.198 0.000 -13.716
ENSG00000137872 E030 0.4439371 0.021817820 3.889517e-01 5.330276e-01 15 47765822 47765868 47 + 0.198 0.000 -13.719
ENSG00000137872 E031 2.3595704 0.007395946 9.966000e-01 1.000000e+00 15 47765869 47766009 141 + 0.494 0.505 0.054
ENSG00000137872 E032 3.1106910 0.005408745 4.911205e-01 6.284197e-01 15 47766105 47766182 78 + 0.611 0.505 -0.487
ENSG00000137872 E033 0.4783925 0.022805932 7.877874e-01 8.640653e-01 15 47766577 47766579 3 + 0.142 0.190 0.514
ENSG00000137872 E034 0.9297165 0.016428450 7.195703e-01 8.143558e-01 15 47766580 47766615 36 + 0.248 0.322 0.512
ENSG00000137872 E035 2.5676793 0.042621737 4.145404e-01 5.579521e-01 15 47766616 47766677 62 + 0.568 0.422 -0.717
ENSG00000137872 E036 1.9420378 0.116861158 4.071832e-01 5.507850e-01 15 47767037 47767093 57 + 0.495 0.319 -0.970
ENSG00000137872 E037 0.1451727 0.044345267 1.000000e+00   15 47767094 47767515 422 + 0.076 0.000 -12.467
ENSG00000137872 E038 1.9324608 0.008142538 8.371566e-02 1.653600e-01 15 47768581 47768615 35 + 0.520 0.190 -2.073
ENSG00000137872 E039 2.7386477 0.006923974 6.696781e-01 7.763834e-01 15 47768616 47768748 133 + 0.568 0.505 -0.295
ENSG00000137872 E040 49.6889016 0.005746056 1.603741e-06 1.273734e-05 15 47770497 47774228 3732 + 1.595 1.803 0.708