ENSG00000137831

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000322954 ENSG00000137831 HEK293_OSMI2_2hA HEK293_TMG_2hB UACA protein_coding protein_coding 10.05892 5.612363 12.6308 0.600289 1.136529 1.168839 1.1876126 0.7281534 1.9668848 0.11233180 0.1306412 1.421236 0.11966667 0.13040000 0.16000000 0.02960000 7.218010e-01 3.7292e-18 FALSE TRUE
ENST00000379983 ENSG00000137831 HEK293_OSMI2_2hA HEK293_TMG_2hB UACA protein_coding protein_coding 10.05892 5.612363 12.6308 0.600289 1.136529 1.168839 1.2892113 0.0000000 1.4252058 0.00000000 0.9904171 7.165114 0.10881667 0.00000000 0.11100000 0.11100000 3.076264e-01 3.7292e-18 FALSE TRUE
ENST00000539319 ENSG00000137831 HEK293_OSMI2_2hA HEK293_TMG_2hB UACA protein_coding protein_coding 10.05892 5.612363 12.6308 0.600289 1.136529 1.168839 3.6973339 1.8605875 4.6281874 0.36523331 0.9202804 1.310070 0.36602917 0.32946667 0.36066667 0.03120000 8.683635e-01 3.7292e-18 FALSE TRUE
ENST00000558308 ENSG00000137831 HEK293_OSMI2_2hA HEK293_TMG_2hB UACA protein_coding retained_intron 10.05892 5.612363 12.6308 0.600289 1.136529 1.168839 0.3686403 0.0000000 0.9360629 0.00000000 0.9360629 6.563864 0.02792917 0.00000000 0.06363333 0.06363333 9.463290e-01 3.7292e-18 FALSE TRUE
ENST00000560441 ENSG00000137831 HEK293_OSMI2_2hA HEK293_TMG_2hB UACA protein_coding protein_coding 10.05892 5.612363 12.6308 0.600289 1.136529 1.168839 0.5459264 0.1182918 0.8782140 0.11829178 0.8782140 2.791479 0.05802083 0.02566667 0.08140000 0.05573333 9.444731e-01 3.7292e-18 FALSE TRUE
ENST00000560523 ENSG00000137831 HEK293_OSMI2_2hA HEK293_TMG_2hB UACA protein_coding retained_intron 10.05892 5.612363 12.6308 0.600289 1.136529 1.168839 0.5331452 0.0000000 0.8187218 0.00000000 0.5520429 6.372816 0.03590833 0.00000000 0.06840000 0.06840000 3.368909e-01 3.7292e-18 FALSE TRUE
ENST00000560831 ENSG00000137831 HEK293_OSMI2_2hA HEK293_TMG_2hB UACA protein_coding processed_transcript 10.05892 5.612363 12.6308 0.600289 1.136529 1.168839 1.1873946 2.7115972 0.0000000 0.56456974 0.0000000 -8.088310 0.17502500 0.47850000 0.00000000 -0.47850000 3.729200e-18 3.7292e-18   FALSE
MSTRG.11134.9 ENSG00000137831 HEK293_OSMI2_2hA HEK293_TMG_2hB UACA protein_coding   10.05892 5.612363 12.6308 0.600289 1.136529 1.168839 0.6273906 0.0448588 1.2956864 0.02293877 0.5236541 4.572942 0.05021250 0.00880000 0.09793333 0.08913333 2.279681e-03 3.7292e-18 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000137831 E001 64.6421842 0.0030019587 8.677926e-03 2.547861e-02 15 70654554 70656801 2248 - 1.694 1.887 0.651
ENSG00000137831 E002 9.7400937 0.0020546785 3.389202e-02 7.953868e-02 15 70656802 70656930 129 - 1.012 0.780 -0.888
ENSG00000137831 E003 1.4987540 0.0855447732 3.345295e-01 4.782775e-01 15 70656931 70656931 1 - 0.401 0.212 -1.273
ENSG00000137831 E004 1.6470472 0.0637325993 7.249509e-01 8.183170e-01 15 70656932 70656933 2 - 0.401 0.352 -0.279
ENSG00000137831 E005 1.6470472 0.0637325993 7.249509e-01 8.183170e-01 15 70656934 70656934 1 - 0.401 0.352 -0.279
ENSG00000137831 E006 10.1007606 0.0220273068 1.011424e-02 2.904257e-02 15 70656935 70656976 42 - 0.860 1.225 1.338
ENSG00000137831 E007 46.3111915 0.0115736563 8.849979e-09 1.101651e-07 15 70656977 70657127 151 - 1.442 1.908 1.583
ENSG00000137831 E008 51.0924871 0.0126089373 6.064439e-08 6.423762e-07 15 70660151 70660216 66 - 1.494 1.939 1.508
ENSG00000137831 E009 1.7296599 0.0100533803 1.468921e-02 3.978764e-02 15 70660217 70661696 1480 - 0.224 0.678 2.479
ENSG00000137831 E010 78.2796473 0.0072462515 1.013506e-13 2.879077e-12 15 70664662 70664814 153 - 1.662 2.132 1.581
ENSG00000137831 E011 0.1817044 0.0406470765 1.383922e-01   15 70664815 70664944 130 - 0.000 0.211 12.066
ENSG00000137831 E012 417.2094667 0.0029719524 6.210724e-06 4.328133e-05 15 70666724 70668917 2194 - 2.505 2.675 0.566
ENSG00000137831 E013 59.3048212 0.0011569457 8.425449e-06 5.693106e-05 15 70668918 70669093 176 - 1.754 1.543 -0.717
ENSG00000137831 E014 34.1720845 0.0033670994 8.858110e-04 3.564524e-03 15 70669094 70669152 59 - 1.524 1.311 -0.735
ENSG00000137831 E015 69.0308216 0.0004088300 2.311507e-06 1.773769e-05 15 70669153 70669462 310 - 1.819 1.621 -0.670
ENSG00000137831 E016 25.5206486 0.0040627668 5.475143e-02 1.175620e-01 15 70671039 70671091 53 - 1.386 1.269 -0.409
ENSG00000137831 E017 0.9286724 0.0147960368 6.954813e-01 7.960658e-01 15 70671092 70671877 786 - 0.266 0.211 -0.430
ENSG00000137831 E018 0.7406253 0.0153430315 2.162375e-01 3.458818e-01 15 70671878 70671964 87 - 0.266 0.000 -12.973
ENSG00000137831 E019 17.2926096 0.0186015187 1.160341e-01 2.142944e-01 15 70671965 70671970 6 - 1.232 1.089 -0.507
ENSG00000137831 E020 19.5028295 0.0172728499 8.781942e-02 1.718143e-01 15 70671971 70672001 31 - 1.280 1.133 -0.524
ENSG00000137831 E021 0.7792333 0.0332557861 8.695728e-01 9.201484e-01 15 70676307 70676492 186 - 0.224 0.211 -0.108
ENSG00000137831 E022 30.6224327 0.0171577737 5.767686e-02 1.226411e-01 15 70676493 70676591 99 - 1.468 1.324 -0.501
ENSG00000137831 E023 21.3785947 0.0009605914 5.519623e-02 1.183280e-01 15 70677108 70677140 33 - 1.310 1.188 -0.429
ENSG00000137831 E024 36.5302288 0.0008238487 5.787646e-05 3.210224e-04 15 70678099 70678206 108 - 1.558 1.310 -0.854
ENSG00000137831 E025 36.2298416 0.0006009768 1.257156e-05 8.165543e-05 15 70679608 70679676 69 - 1.560 1.283 -0.957
ENSG00000137831 E026 1.7253960 0.0082964941 7.656626e-01 8.483593e-01 15 70679677 70680034 358 - 0.371 0.460 0.478
ENSG00000137831 E027 0.7384352 0.0216076509 2.167947e-01 3.465527e-01 15 70681537 70682028 492 - 0.266 0.000 -12.905
ENSG00000137831 E028 29.0364840 0.0029713941 7.671806e-05 4.125346e-04 15 70682758 70682795 38 - 1.468 1.171 -1.039
ENSG00000137831 E029 33.2182998 0.0006309873 3.914997e-04 1.742030e-03 15 70684265 70684356 92 - 1.515 1.297 -0.756
ENSG00000137831 E030 30.2599158 0.0007583620 1.225919e-04 6.257318e-04 15 70684357 70684446 90 - 1.483 1.222 -0.907
ENSG00000137831 E031 37.1602320 0.0005945318 2.941880e-04 1.356200e-03 15 70687540 70687646 107 - 1.558 1.349 -0.720
ENSG00000137831 E032 28.1649918 0.0006816572 4.311728e-04 1.894219e-03 15 70687750 70687820 71 - 1.448 1.205 -0.845
ENSG00000137831 E033 23.3664690 0.0008198816 2.354487e-04 1.114458e-03 15 70690454 70690511 58 - 1.380 1.089 -1.025
ENSG00000137831 E034 21.5404576 0.0008479452 6.801616e-04 2.826626e-03 15 70691299 70691363 65 - 1.341 1.067 -0.974
ENSG00000137831 E035 26.4067482 0.0007003202 1.826346e-03 6.684617e-03 15 70695017 70695105 89 - 1.418 1.205 -0.742
ENSG00000137831 E036 28.4306833 0.0008589557 1.862124e-03 6.795875e-03 15 70699527 70699660 134 - 1.442 1.238 -0.710
ENSG00000137831 E037 0.1472490 0.0442550901 1.000000e+00   15 70699661 70699666 6 - 0.068 0.000 -10.444
ENSG00000137831 E038 0.2903454 0.2880747501 1.000000e+00   15 70701877 70702308 432 - 0.126 0.000 -10.444
ENSG00000137831 E039 0.0000000       15 70703141 70703275 135 -      
ENSG00000137831 E040 15.9869840 0.0306551403 3.648711e-01 5.092101e-01 15 70763330 70763558 229 - 1.177 1.116 -0.218