ENSG00000137825

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000260386 ENSG00000137825 HEK293_OSMI2_2hA HEK293_TMG_2hB ITPKA protein_coding protein_coding 5.13063 9.963482 2.656233 0.5673825 0.05291186 -1.903295 2.2439558 4.0540066 1.0715554 0.44874982 0.2282222 -1.90979523 0.4181958 0.4073333 0.4053333 -0.0020000 1.000000e+00 1.826147e-06 FALSE TRUE
ENST00000425927 ENSG00000137825 HEK293_OSMI2_2hA HEK293_TMG_2hB ITPKA protein_coding protein_coding 5.13063 9.963482 2.656233 0.5673825 0.05291186 -1.903295 0.6078778 0.8449145 0.8549250 0.09376524 0.1140535 0.01679478 0.1435750 0.0848000 0.3226333 0.2378333 1.826147e-06 1.826147e-06 FALSE FALSE
ENST00000462816 ENSG00000137825 HEK293_OSMI2_2hA HEK293_TMG_2hB ITPKA protein_coding retained_intron 5.13063 9.963482 2.656233 0.5673825 0.05291186 -1.903295 2.1791100 4.8250501 0.7297522 0.41611702 0.3661036 -2.70841680 0.4217667 0.4845667 0.2720333 -0.2125333 6.270279e-01 1.826147e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000137825 E001 6.9034119 0.0026244224 0.063172310 0.13199299 15 41493393 41493494 102 + 0.975 0.730 -0.951
ENSG00000137825 E002 22.1049309 0.0008753354 0.260059677 0.39754194 15 41493874 41494272 399 + 1.301 1.206 -0.334
ENSG00000137825 E003 39.1238211 0.0006363068 0.356447141 0.50081830 15 41494273 41494416 144 + 1.504 1.447 -0.198
ENSG00000137825 E004 0.4720498 0.1761201784 0.082884884 0.16406115 15 41501439 41501462 24 + 0.381 0.061 -3.217
ENSG00000137825 E005 35.5394173 0.0007963815 0.103810841 0.19631708 15 41501463 41501559 97 + 1.504 1.395 -0.378
ENSG00000137825 E006 44.3731783 0.0006603802 0.091026803 0.17675688 15 41501635 41501793 159 + 1.591 1.489 -0.347
ENSG00000137825 E007 32.0183734 0.0007330357 0.538182730 0.66962966 15 41501794 41501851 58 + 1.408 1.367 -0.143
ENSG00000137825 E008 65.6001038 0.0004724938 0.613840951 0.73245714 15 41501997 41502201 205 + 1.695 1.674 -0.069
ENSG00000137825 E009 52.2430157 0.0005367017 0.110294944 0.20598226 15 41502402 41502503 102 + 1.485 1.596 0.379
ENSG00000137825 E010 1.0965559 0.0487656298 0.291494884 0.43236742 15 41502504 41502645 142 + 0.001 0.280 9.297
ENSG00000137825 E011 47.2685311 0.0005252182 0.007346843 0.02212004 15 41502788 41502859 72 + 1.358 1.559 0.694
ENSG00000137825 E012 117.8215048 0.0066942885 0.145928253 0.25613258 15 41502963 41503551 589 + 1.858 1.941 0.280