ENSG00000137812

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000399668 ENSG00000137812 HEK293_OSMI2_2hA HEK293_TMG_2hB KNL1 protein_coding protein_coding 6.53868 3.702911 8.718687 0.7150582 0.3553742 1.233214 4.4455967 0.4978022 7.8780210 0.08255663 0.2006576 3.9573248 0.5367792 0.1461333 0.9047667 0.7586333 5.644824e-18 5.644824e-18 FALSE TRUE
ENST00000526913 ENSG00000137812 HEK293_OSMI2_2hA HEK293_TMG_2hB KNL1 protein_coding nonsense_mediated_decay 6.53868 3.702911 8.718687 0.7150582 0.3553742 1.233214 0.6537763 0.3607693 0.2339432 0.09741059 0.2339432 -0.6039763 0.1207042 0.1114000 0.0259000 -0.0855000 1.890695e-01 5.644824e-18 TRUE TRUE
ENST00000531626 ENSG00000137812 HEK293_OSMI2_2hA HEK293_TMG_2hB KNL1 protein_coding processed_transcript 6.53868 3.702911 8.718687 0.7150582 0.3553742 1.233214 0.9025662 2.8116919 0.0000000 0.84211801 0.0000000 -8.1404166 0.2466500 0.7313667 0.0000000 -0.7313667 2.115504e-17 5.644824e-18   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000137812 E001 0.2966881 0.0290785164 1.000000e+00   15 40594020 40594033 14 + 0.089 0.000 -9.496
ENSG00000137812 E002 0.2966881 0.0290785164 1.000000e+00   15 40594034 40594034 1 + 0.089 0.000 -10.176
ENSG00000137812 E003 1.6607648 0.0084064749 7.907293e-01 8.661688e-01 15 40594035 40594248 214 + 0.331 0.285 -0.301
ENSG00000137812 E004 0.9180632 0.0133415057 7.104053e-01 8.075905e-01 15 40594249 40594264 16 + 0.196 0.285 0.700
ENSG00000137812 E005 1.9489201 0.0142899312 6.435271e-01 7.560481e-01 15 40594265 40594271 7 + 0.375 0.285 -0.563
ENSG00000137812 E006 20.1058952 0.0010003402 4.017898e-05 2.314950e-04 15 40594272 40594392 121 + 1.206 0.578 -2.438
ENSG00000137812 E007 17.4311116 0.0084393355 7.376251e-05 3.981902e-04 15 40602915 40602966 52 + 1.151 0.456 -2.825
ENSG00000137812 E008 17.2648344 0.0013534994 4.584068e-05 2.606826e-04 15 40605110 40605149 40 + 1.147 0.456 -2.812
ENSG00000137812 E009 14.3427421 0.0011679530 1.687067e-06 1.333819e-05 15 40606393 40606432 40 + 1.082 0.000 -15.340
ENSG00000137812 E010 13.8903859 0.0012221411 2.497932e-06 1.903410e-05 15 40606433 40606452 20 + 1.070 0.000 -15.299
ENSG00000137812 E011 15.2285401 0.0011068619 7.776101e-07 6.613509e-06 15 40608847 40608908 62 + 1.106 0.000 -15.418
ENSG00000137812 E012 14.1075647 0.0012237624 4.916753e-05 2.775353e-04 15 40610245 40610297 53 + 1.070 0.285 -3.533
ENSG00000137812 E013 0.2987644 0.0295014058 1.000000e+00   15 40610799 40610876 78 + 0.089 0.000 -10.177
ENSG00000137812 E014 10.4120528 0.0017630602 7.029738e-03 2.130241e-02 15 40611478 40611483 6 + 0.943 0.456 -2.066
ENSG00000137812 E015 12.3266314 0.0017620322 1.771930e-03 6.509975e-03 15 40611484 40611511 28 + 1.011 0.456 -2.319
ENSG00000137812 E016 9.3779737 0.0052590396 5.976817e-02 1.262195e-01 15 40615341 40615378 38 + 0.895 0.578 -1.301
ENSG00000137812 E017 15.1730027 0.0012533170 5.430938e-03 1.710117e-02 15 40618959 40619011 53 + 1.086 0.673 -1.594
ENSG00000137812 E018 253.0923978 0.0565127918 8.480963e-06 5.727757e-05 15 40620640 40623131 2492 + 2.271 1.801 -1.579
ENSG00000137812 E019 268.6983091 0.0042941876 6.621755e-07 5.716956e-06 15 40623132 40625640 2509 + 2.278 2.087 -0.638
ENSG00000137812 E020 1.7254091 0.0329332499 1.733157e-01 2.925045e-01 15 40625641 40626042 402 + 0.282 0.578 1.608
ENSG00000137812 E021 14.1977017 0.0014311607 5.381851e-01 6.696297e-01 15 40628070 40628073 4 + 1.025 0.971 -0.201
ENSG00000137812 E022 36.1723682 0.0055390003 6.288499e-01 7.443252e-01 15 40628074 40628208 135 + 1.401 1.385 -0.057
ENSG00000137812 E023 30.3596088 0.0128161267 8.215748e-01 8.874738e-01 15 40628611 40628678 68 + 1.329 1.332 0.013
ENSG00000137812 E024 28.9036155 0.0020792781 9.683604e-01 9.842426e-01 15 40629273 40629323 51 + 1.305 1.331 0.090
ENSG00000137812 E025 29.4196500 0.0055737116 3.160598e-01 4.588254e-01 15 40629324 40629371 48 + 1.324 1.249 -0.264
ENSG00000137812 E026 41.1776237 0.0005664659 9.032124e-03 2.635922e-02 15 40640912 40641027 116 + 1.477 1.270 -0.719
ENSG00000137812 E027 38.4386667 0.0006090095 3.594336e-01 5.037666e-01 15 40644997 40645087 91 + 1.432 1.384 -0.168
ENSG00000137812 E028 45.1622006 0.0006244216 2.111004e-01 3.398328e-01 15 40645656 40645772 117 + 1.501 1.431 -0.242
ENSG00000137812 E029 0.0000000       15 40646886 40646986 101 +      
ENSG00000137812 E030 52.4432084 0.0005441513 2.683144e-01 4.067604e-01 15 40646987 40647074 88 + 1.564 1.512 -0.176
ENSG00000137812 E031 0.5997190 0.1578298281 1.000000e+00 1.000000e+00 15 40650127 40650300 174 + 0.164 0.000 -11.311
ENSG00000137812 E032 44.8546648 0.0010651577 3.051385e-01 4.471848e-01 15 40650301 40650378 78 + 1.498 1.446 -0.181
ENSG00000137812 E033 34.6520812 0.0006304360 7.325871e-01 8.240300e-01 15 40650544 40650583 40 + 1.384 1.384 0.000
ENSG00000137812 E034 47.0628440 0.0016201643 9.937331e-01 1.000000e+00 15 40651471 40651572 102 + 1.508 1.537 0.100
ENSG00000137812 E035 45.0652854 0.0029831101 6.438327e-01 7.562853e-01 15 40652005 40652105 101 + 1.495 1.487 -0.026
ENSG00000137812 E036 35.7347406 0.0211339747 6.235512e-01 7.401159e-01 15 40654909 40654977 69 + 1.387 1.462 0.261
ENSG00000137812 E037 35.4124618 0.0187357527 6.381269e-01 7.518516e-01 15 40657042 40657151 110 + 1.397 1.369 -0.096
ENSG00000137812 E038 24.7560650 0.0188918011 7.782892e-01 8.573405e-01 15 40657355 40657473 119 + 1.238 1.292 0.190
ENSG00000137812 E039 38.8673198 0.0005652809 1.586494e-01 2.733301e-01 15 40659339 40659461 123 + 1.409 1.548 0.476
ENSG00000137812 E040 102.5441076 0.0039225444 5.071218e-04 2.186850e-03 15 40662074 40662572 499 + 1.809 2.025 0.724
ENSG00000137812 E041 38.4781035 0.0005839177 4.072629e-05 2.342978e-04 15 40662573 40662678 106 + 1.373 1.692 1.092
ENSG00000137812 E042 18.6325163 0.0072299435 4.259502e-03 1.386822e-02 15 40662679 40662683 5 + 1.074 1.399 1.151
ENSG00000137812 E043 78.3640866 0.0003919823 1.476348e-04 7.369175e-04 15 40662684 40663334 651 + 1.694 1.922 0.769
ENSG00000137812 E044 18.1157427 0.0567608394 1.222885e-02 3.408342e-02 15 40663335 40663341 7 + 1.044 1.460 1.470
ENSG00000137812 E045 142.4234205 0.0582450284 5.902316e-09 7.607675e-08 15 40663342 40664342 1001 + 1.808 2.499 2.314