ENSG00000137574

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000260129 ENSG00000137574 HEK293_OSMI2_2hA HEK293_TMG_2hB TGS1 protein_coding protein_coding 12.9602 3.271034 23.66181 0.1895794 0.8784691 2.850948 9.759632 3.114984 16.90509 0.1494066 0.3602017 2.436390 0.8318333 0.9534333 0.7155667 -0.2378667 1.076260e-10 3.293488e-15 FALSE TRUE
ENST00000519494 ENSG00000137574 HEK293_OSMI2_2hA HEK293_TMG_2hB TGS1 protein_coding retained_intron 12.9602 3.271034 23.66181 0.1895794 0.8784691 2.850948 2.729894 0.000000 5.91203 0.0000000 0.7624585 9.209948 0.1171125 0.0000000 0.2486333 0.2486333 3.293488e-15 3.293488e-15 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000137574 E001 7.6127336 0.0022039608 2.588207e-02 6.369965e-02 8 55773446 55773469 24 + 0.876 0.524 -1.477
ENSG00000137574 E002 69.4158423 0.0011197108 1.789546e-02 4.691376e-02 8 55773470 55773667 198 + 1.742 1.643 -0.334
ENSG00000137574 E003 63.2818658 0.0004634607 3.695462e-04 1.656283e-03 8 55773668 55773719 52 + 1.714 1.529 -0.631
ENSG00000137574 E004 75.7631581 0.0003406101 1.036464e-05 6.865556e-05 8 55782748 55782812 65 + 1.796 1.576 -0.746
ENSG00000137574 E005 92.2316740 0.0002803646 5.556461e-05 3.094769e-04 8 55785719 55785891 173 + 1.875 1.707 -0.567
ENSG00000137574 E006 0.1515154 0.0428722037 1.000000e+00   8 55786221 55786237 17 + 0.055 0.001 -6.797
ENSG00000137574 E007 351.0746741 0.0003475612 1.912974e-07 1.840191e-06 8 55786238 55787060 823 + 2.439 2.351 -0.292
ENSG00000137574 E008 0.1515154 0.0428722037 1.000000e+00   8 55790097 55790181 85 + 0.055 0.001 -6.797
ENSG00000137574 E009 130.3864556 0.0005975643 1.548572e-03 5.793062e-03 8 55790182 55790299 118 + 2.012 1.919 -0.313
ENSG00000137574 E010 105.7859503 0.0004169748 1.571493e-03 5.866297e-03 8 55792698 55792784 87 + 1.925 1.818 -0.361
ENSG00000137574 E011 135.3852613 0.0002188510 1.627300e-04 8.037308e-04 8 55795978 55796152 175 + 2.033 1.919 -0.383
ENSG00000137574 E012 192.5480961 0.0002651350 4.901908e-01 6.275395e-01 8 55798914 55799220 307 + 2.161 2.183 0.072
ENSG00000137574 E013 135.3717653 0.0010528073 2.873914e-01 4.278889e-01 8 55802457 55802606 150 + 1.997 2.084 0.290
ENSG00000137574 E014 130.7510462 0.0007799073 6.491457e-02 1.348939e-01 8 55804893 55805036 144 + 1.978 2.094 0.390
ENSG00000137574 E015 113.7053392 0.0002461191 2.806567e-02 6.814427e-02 8 55810881 55811097 217 + 1.913 2.045 0.446
ENSG00000137574 E016 34.1185706 0.0007897076 2.096705e-02 5.352142e-02 8 55813040 55813054 15 + 1.386 1.593 0.711
ENSG00000137574 E017 70.9161658 0.0004075235 7.364620e-03 2.216476e-02 8 55813055 55813118 64 + 1.701 1.880 0.604
ENSG00000137574 E018 280.1752767 0.0087858625 2.137798e-12 4.969318e-11 8 55824581 55826445 1865 + 2.242 2.601 1.194