ENSG00000137221

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000259751 ENSG00000137221 HEK293_OSMI2_2hA HEK293_TMG_2hB TJAP1 protein_coding protein_coding 33.51173 46.13269 27.04748 3.711588 0.9257658 -0.7700746 3.645195 4.595643 2.546500 0.8327317 0.34511919 -0.8492325 0.10949583 0.10403333 0.09443333 -0.00960000 9.692501e-01 7.347481e-23 FALSE  
ENST00000372444 ENSG00000137221 HEK293_OSMI2_2hA HEK293_TMG_2hB TJAP1 protein_coding protein_coding 33.51173 46.13269 27.04748 3.711588 0.9257658 -0.7700746 2.877242 4.300295 2.072959 0.2099703 0.33789534 -1.0491520 0.08667917 0.09413333 0.07633333 -0.01780000 6.273580e-01 7.347481e-23 FALSE  
ENST00000372452 ENSG00000137221 HEK293_OSMI2_2hA HEK293_TMG_2hB TJAP1 protein_coding protein_coding 33.51173 46.13269 27.04748 3.711588 0.9257658 -0.7700746 6.441355 10.299991 4.849591 1.7853101 1.32676791 -1.0851364 0.18115000 0.22066667 0.17653333 -0.04413333 7.141382e-01 7.347481e-23 FALSE  
ENST00000454762 ENSG00000137221 HEK293_OSMI2_2hA HEK293_TMG_2hB TJAP1 protein_coding protein_coding 33.51173 46.13269 27.04748 3.711588 0.9257658 -0.7700746 1.388265 2.438552 0.000000 0.3314044 0.00000000 -7.9357852 0.03690833 0.05240000 0.00000000 -0.05240000 7.347481e-23 7.347481e-23 FALSE  
ENST00000490050 ENSG00000137221 HEK293_OSMI2_2hA HEK293_TMG_2hB TJAP1 protein_coding processed_transcript 33.51173 46.13269 27.04748 3.711588 0.9257658 -0.7700746 1.759025 0.852129 1.808063 0.1334368 0.05142815 1.0764264 0.05956667 0.01826667 0.06696667 0.04870000 5.633909e-11 7.347481e-23 TRUE  
MSTRG.28329.18 ENSG00000137221 HEK293_OSMI2_2hA HEK293_TMG_2hB TJAP1 protein_coding   33.51173 46.13269 27.04748 3.711588 0.9257658 -0.7700746 3.243782 2.841703 2.621714 0.5381525 0.09333837 -0.1158206 0.10409167 0.06126667 0.09736667 0.03610000 1.292241e-01 7.347481e-23 FALSE  
MSTRG.28329.4 ENSG00000137221 HEK293_OSMI2_2hA HEK293_TMG_2hB TJAP1 protein_coding   33.51173 46.13269 27.04748 3.711588 0.9257658 -0.7700746 6.663745 13.885862 4.472609 2.4014310 1.71814676 -1.6322449 0.17827083 0.29690000 0.16966667 -0.12723333 4.584789e-01 7.347481e-23 FALSE  
MSTRG.28329.7 ENSG00000137221 HEK293_OSMI2_2hA HEK293_TMG_2hB TJAP1 protein_coding   33.51173 46.13269 27.04748 3.711588 0.9257658 -0.7700746 1.581285 0.000000 1.656230 0.0000000 0.83304463 7.3804436 0.05933750 0.00000000 0.06066667 0.06066667 1.377403e-01 7.347481e-23 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000137221 E001 0.0000000       6 43477523 43477526 4 +      
ENSG00000137221 E002 0.0000000       6 43477527 43477528 2 +      
ENSG00000137221 E003 0.6664265 0.0164481527 5.054496e-01 6.412240e-01 6 43477529 43477533 5 + 0.276 0.169 -0.895
ENSG00000137221 E004 0.6664265 0.0164481527 5.054496e-01 6.412240e-01 6 43477534 43477535 2 + 0.276 0.169 -0.895
ENSG00000137221 E005 1.1909026 0.1210630113 2.658616e-01 4.039676e-01 6 43477536 43477555 20 + 0.444 0.236 -1.299
ENSG00000137221 E006 1.1909026 0.1210630113 2.658616e-01 4.039676e-01 6 43477556 43477556 1 + 0.444 0.236 -1.299
ENSG00000137221 E007 1.3360753 0.0670197139 1.253946e-01 2.277080e-01 6 43477557 43477565 9 + 0.508 0.235 -1.625
ENSG00000137221 E008 1.7392248 0.0381034803 3.159623e-01 4.587391e-01 6 43477566 43477568 3 + 0.508 0.343 -0.887
ENSG00000137221 E009 1.8875180 0.0158442135 4.299511e-01 5.725950e-01 6 43477569 43477572 4 + 0.508 0.386 -0.630
ENSG00000137221 E010 2.0326907 0.0192355236 2.610377e-01 3.986523e-01 6 43477573 43477574 2 + 0.564 0.386 -0.892
ENSG00000137221 E011 74.6645237 0.0003693422 6.508641e-01 7.618513e-01 6 43477575 43477628 54 + 1.835 1.841 0.018
ENSG00000137221 E012 0.1817044 0.0398888698 1.000000e+00   6 43477629 43477632 4 + 0.000 0.093 9.368
ENSG00000137221 E013 44.7993577 0.0032873628 1.631258e-02 4.341626e-02 6 43477874 43478086 213 + 1.701 1.584 -0.400
ENSG00000137221 E014 114.8981724 0.0058885969 2.151036e-01 3.445626e-01 6 43478087 43478136 50 + 2.049 2.016 -0.110
ENSG00000137221 E015 190.6566127 0.0011373909 1.830299e-03 6.696303e-03 6 43478137 43478232 96 + 2.289 2.220 -0.229
ENSG00000137221 E016 35.2297603 0.0008202864 6.004086e-01 7.216459e-01 6 43478659 43478718 60 + 1.524 1.515 -0.028
ENSG00000137221 E017 0.1482932 0.0421509027 1.000000e+00   6 43489584 43489614 31 + 0.000 0.093 9.342
ENSG00000137221 E018 0.2955422 0.0298164035 6.204312e-01   6 43489615 43489803 189 + 0.159 0.093 -0.897
ENSG00000137221 E019 1.0329427 0.0380887995 3.679171e-01 5.123061e-01 6 43496988 43497880 893 + 0.159 0.341 1.426
ENSG00000137221 E020 233.4191728 0.0023055003 1.258724e-02 3.493890e-02 6 43497881 43497977 97 + 2.368 2.314 -0.180
ENSG00000137221 E021 19.8505073 0.0253627083 9.775510e-01 9.900372e-01 6 43498923 43498974 52 + 1.273 1.298 0.087
ENSG00000137221 E022 68.2551814 0.0008399095 5.390212e-01 6.703381e-01 6 43498975 43498977 3 + 1.800 1.797 -0.010
ENSG00000137221 E023 167.5506388 0.0022698376 2.007766e-01 3.271650e-01 6 43498978 43499019 42 + 2.198 2.180 -0.059
ENSG00000137221 E024 217.5335041 0.0014136132 5.376957e-01 6.692168e-01 6 43499020 43499096 77 + 2.293 2.302 0.030
ENSG00000137221 E025 118.8418606 0.0003571222 8.132670e-01 8.815895e-01 6 43499097 43499100 4 + 2.028 2.045 0.057
ENSG00000137221 E026 10.5910278 0.0261253618 5.523707e-01 6.816552e-01 6 43499101 43499136 36 + 1.067 1.014 -0.194
ENSG00000137221 E027 8.7923859 0.0105810713 9.173513e-05 4.831405e-04 6 43500609 43500743 135 + 1.207 0.779 -1.591
ENSG00000137221 E028 144.5682684 0.0002870701 6.433742e-01 7.559480e-01 6 43500744 43500772 29 + 2.116 2.127 0.036
ENSG00000137221 E029 23.0852119 0.0008291035 3.881969e-15 1.358632e-13 6 43500773 43500965 193 + 1.639 1.104 -1.865
ENSG00000137221 E030 37.2311880 0.0005800212 4.496636e-24 4.490639e-22 6 43500966 43501525 560 + 1.844 1.287 -1.903
ENSG00000137221 E031 286.9347389 0.0018287356 3.772902e-01 5.216256e-01 6 43501526 43501687 162 + 2.418 2.421 0.011
ENSG00000137221 E032 22.6633776 0.0008435924 1.931869e-09 2.713511e-08 6 43502121 43502282 162 + 1.573 1.165 -1.419
ENSG00000137221 E033 217.8519701 0.0002424284 2.742687e-03 9.500188e-03 6 43502283 43502349 67 + 2.333 2.282 -0.170
ENSG00000137221 E034 2.9463818 0.0065637011 1.902116e-01 3.140415e-01 6 43502588 43502617 30 + 0.368 0.614 1.223
ENSG00000137221 E035 6.7222662 0.0024092136 4.917201e-02 1.076848e-01 6 43502618 43503400 783 + 0.995 0.779 -0.825
ENSG00000137221 E036 300.1160331 0.0002835194 2.725736e-03 9.449768e-03 6 43503401 43503508 108 + 2.465 2.425 -0.134
ENSG00000137221 E037 247.9213720 0.0002302005 4.012380e-02 9.126455e-02 6 43503623 43503706 84 + 2.372 2.349 -0.079
ENSG00000137221 E038 105.7097171 0.0002601969 5.900390e-22 4.673373e-20 6 43503707 43504667 961 + 2.178 1.870 -1.033
ENSG00000137221 E039 26.4372956 0.0194923690 6.385626e-03 1.962554e-02 6 43504668 43504760 93 + 1.541 1.315 -0.778
ENSG00000137221 E040 893.9580113 0.0006537343 6.303872e-08 6.656910e-07 6 43504761 43505660 900 + 2.832 2.946 0.380
ENSG00000137221 E041 942.8202347 0.0022018964 3.186076e-12 7.205355e-11 6 43505661 43506448 788 + 2.801 2.989 0.625
ENSG00000137221 E042 149.5019278 0.0051091533 1.872404e-06 1.465985e-05 6 43506449 43506556 108 + 1.951 2.207 0.859