ENSG00000137193

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000373509 ENSG00000137193 HEK293_OSMI2_2hA HEK293_TMG_2hB PIM1 protein_coding protein_coding 8.104879 10.16883 7.306387 0.3906054 0.2057391 -0.476369 6.3320513 5.6604120 7.1513226 0.6073942 0.2174221 0.3367725 0.80647083 0.55560000 0.97890000 0.42330000 9.341445e-05 4.096308e-20 FALSE TRUE
ENST00000479509 ENSG00000137193 HEK293_OSMI2_2hA HEK293_TMG_2hB PIM1 protein_coding processed_transcript 8.104879 10.16883 7.306387 0.3906054 0.2057391 -0.476369 0.9705326 3.4844624 0.0000000 0.3485989 0.0000000 -8.4489267 0.10272917 0.34640000 0.00000000 -0.34640000 4.096308e-20 4.096308e-20 FALSE TRUE
MSTRG.28222.2 ENSG00000137193 HEK293_OSMI2_2hA HEK293_TMG_2hB PIM1 protein_coding   8.104879 10.16883 7.306387 0.3906054 0.2057391 -0.476369 0.6811903 0.9996123 0.1270407 0.6646402 0.1270407 -2.8811254 0.07546667 0.09536667 0.01703333 -0.07833333 5.865961e-01 4.096308e-20 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000137193 E001 15.5160688 0.0027174801 3.758918e-04 1.680946e-03 6 37170152 37170393 242 + 1.358 1.078 -0.989
ENSG00000137193 E002 47.2533940 0.0286273200 9.700655e-02 1.859027e-01 6 37170394 37170657 264 + 1.740 1.622 -0.400
ENSG00000137193 E003 47.3317603 0.0128735239 1.751260e-01 2.948446e-01 6 37170773 37170879 107 + 1.711 1.647 -0.215
ENSG00000137193 E004 47.4953444 0.0122128381 8.813984e-02 1.722900e-01 6 37170981 37171031 51 + 1.725 1.638 -0.295
ENSG00000137193 E005 53.5303347 0.0004573824 6.492319e-03 1.990248e-02 6 37171125 37171198 74 + 1.785 1.685 -0.337
ENSG00000137193 E006 113.8407412 0.0021755742 7.802373e-03 2.328140e-02 6 37171199 37171491 293 + 2.089 2.020 -0.232
ENSG00000137193 E007 0.4470576 0.0215238268 4.321730e-01 5.746221e-01 6 37172468 37172493 26 + 0.234 0.113 -1.269
ENSG00000137193 E008 0.2955422 0.0294610419 8.558898e-01   6 37172494 37172612 119 + 0.133 0.113 -0.270
ENSG00000137193 E009 0.6330284 0.0598769108 8.086735e-01 8.784784e-01 6 37172613 37172794 182 + 0.234 0.202 -0.272
ENSG00000137193 E010 94.8734507 0.0003839243 2.440612e-01 3.789778e-01 6 37172996 37173172 177 + 1.980 1.967 -0.045
ENSG00000137193 E011 394.3860281 0.0034011851 4.234782e-09 5.587205e-08 6 37173934 37175428 1495 + 2.495 2.644 0.496