ENSG00000137171

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000347162 ENSG00000137171 HEK293_OSMI2_2hA HEK293_TMG_2hB KLC4 protein_coding protein_coding 20.33776 29.42055 13.85178 1.073572 0.1971835 -1.086202 13.750390 23.9380496 8.9780959 0.90258005 0.1740853 -1.4138207 0.66043750 0.81370000 0.64833333 -0.16536667 3.097518e-07 3.097518e-07 FALSE TRUE
ENST00000481499 ENSG00000137171 HEK293_OSMI2_2hA HEK293_TMG_2hB KLC4 protein_coding nonsense_mediated_decay 20.33776 29.42055 13.85178 1.073572 0.1971835 -1.086202 1.004920 0.7565621 0.6851366 0.21158239 0.0976389 -0.1411060 0.05116250 0.02606667 0.04930000 0.02323333 2.301943e-01 3.097518e-07 FALSE TRUE
ENST00000481888 ENSG00000137171 HEK293_OSMI2_2hA HEK293_TMG_2hB KLC4 protein_coding protein_coding 20.33776 29.42055 13.85178 1.073572 0.1971835 -1.086202 1.021429 0.5344375 0.6854420 0.05856226 0.2068486 0.3531639 0.05529583 0.01803333 0.04913333 0.03110000 1.157630e-01 3.097518e-07 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000137171 E001 16.1564309 0.0012952730 1.685460e-04 0.0008289553 6 43040777 43041124 348 + 1.388 1.061 -1.158
ENSG00000137171 E002 11.3198885 0.0029550810 7.617451e-05 0.0004099026 6 43043058 43043508 451 + 1.291 0.892 -1.445
ENSG00000137171 E003 1.1040064 0.3500556750 3.083567e-01 0.4506322275 6 43059026 43059271 246 + 0.420 0.209 -1.399
ENSG00000137171 E004 0.5169874 0.0198698201 7.764902e-01 0.8560729169 6 43059405 43059630 226 + 0.189 0.143 -0.487
ENSG00000137171 E005 1.8131183 0.0298365389 5.560253e-01 0.6847689951 6 43059631 43059646 16 + 0.320 0.438 0.674
ENSG00000137171 E006 25.8753869 0.0007564156 1.597573e-04 0.0007908011 6 43059647 43059651 5 + 1.541 1.272 -0.933
ENSG00000137171 E007 39.5122402 0.0006164786 3.615139e-02 0.0838828209 6 43059652 43059652 1 + 1.622 1.493 -0.439
ENSG00000137171 E008 74.2122196 0.0059831296 8.260466e-01 0.8905205095 6 43059653 43059685 33 + 1.804 1.792 -0.040
ENSG00000137171 E009 1.7068589 0.1664798981 8.467203e-02 0.1668698189 6 43059857 43059882 26 + 0.632 0.254 -2.044
ENSG00000137171 E010 1.8801442 0.0324812224 6.169876e-02 0.1294868135 6 43059883 43059886 4 + 0.630 0.296 -1.743
ENSG00000137171 E011 2.2133640 0.1308773034 9.404356e-02 0.1814468934 6 43059887 43059889 3 + 0.683 0.340 -1.683
ENSG00000137171 E012 2.2435530 0.1573875123 2.185945e-01 0.3487303752 6 43059890 43059894 5 + 0.631 0.379 -1.232
ENSG00000137171 E013 14.7446446 0.0012672517 5.004534e-02 0.1092525815 6 43059895 43060159 265 + 1.253 1.068 -0.659
ENSG00000137171 E014 5.4769587 0.0052535065 1.770474e-01 0.2974003045 6 43060160 43060182 23 + 0.877 0.689 -0.749
ENSG00000137171 E015 4.5870107 0.0294770385 2.750194e-01 0.4142675933 6 43060183 43060186 4 + 0.809 0.635 -0.715
ENSG00000137171 E016 7.5688204 0.0456445965 3.851466e-01 0.5293226683 6 43060187 43060238 52 + 0.963 0.835 -0.487
ENSG00000137171 E017 5.7704482 0.0598109072 9.093330e-01 0.9464178821 6 43060239 43060276 38 + 0.771 0.757 -0.056
ENSG00000137171 E018 9.3141751 0.0523633949 1.180028e-01 0.2170751569 6 43060277 43060517 241 + 1.114 0.881 -0.862
ENSG00000137171 E019 8.9937163 0.0022106687 1.086725e-02 0.0308497166 6 43060518 43060605 88 + 1.131 0.847 -1.057
ENSG00000137171 E020 6.6189237 0.0130737237 7.701682e-03 0.0230299182 6 43061062 43061310 249 + 1.055 0.706 -1.343
ENSG00000137171 E021 217.3592134 0.0046880757 3.630482e-01 0.5074771923 6 43061311 43061593 283 + 2.289 2.252 -0.122
ENSG00000137171 E022 7.5533420 0.0023315236 4.864906e-02 0.1067703713 6 43062528 43062885 358 + 1.034 0.796 -0.902
ENSG00000137171 E023 5.9753803 0.0137876765 5.158138e-01 0.6504408002 6 43062886 43062916 31 + 0.683 0.784 0.414
ENSG00000137171 E024 102.7806969 0.0104377662 7.391771e-01 0.8288736251 6 43062917 43062982 66 + 1.920 1.943 0.076
ENSG00000137171 E025 188.5468250 0.0057667673 6.959278e-01 0.7963795913 6 43062983 43063147 165 + 2.212 2.195 -0.056
ENSG00000137171 E026 1.1866362 0.0117798989 8.932098e-02 0.1740976174 6 43065392 43065619 228 + 0.502 0.200 -1.901
ENSG00000137171 E027 130.8301059 0.0071931532 4.704898e-02 0.1039123360 6 43065620 43065701 82 + 2.118 2.015 -0.346
ENSG00000137171 E028 210.0570800 0.0083320445 1.512972e-01 0.2634352467 6 43066306 43066525 220 + 2.301 2.229 -0.241
ENSG00000137171 E029 156.1443348 0.0037534449 3.474946e-01 0.4917188213 6 43066996 43067079 84 + 2.150 2.109 -0.135
ENSG00000137171 E030 96.0568374 0.0007124881 6.309514e-01 0.7460668473 6 43067080 43067083 4 + 1.926 1.906 -0.068
ENSG00000137171 E031 2.8966170 0.0367849586 4.587153e-03 0.0147843210 6 43067084 43067397 314 + 0.845 0.373 -2.142
ENSG00000137171 E032 1.8488108 0.0149709532 4.355536e-03 0.0141364281 6 43070346 43070353 8 + 0.729 0.249 -2.490
ENSG00000137171 E033 187.1083335 0.0005151507 7.930133e-01 0.8676436018 6 43070354 43070455 102 + 2.204 2.197 -0.026
ENSG00000137171 E034 4.0397340 0.0202063627 1.627075e-01 0.2786881806 6 43070456 43070691 236 + 0.809 0.592 -0.907
ENSG00000137171 E035 245.1320211 0.0002063678 6.226254e-01 0.7394015881 6 43070692 43070865 174 + 2.324 2.311 -0.041
ENSG00000137171 E036 177.3797402 0.0038260698 8.586574e-01 0.9128101710 6 43071275 43071374 100 + 2.167 2.177 0.033
ENSG00000137171 E037 129.9703954 0.0125651370 8.979762e-01 0.9388770857 6 43071567 43071619 53 + 2.032 2.043 0.038
ENSG00000137171 E038 161.8987674 0.0062355196 7.493386e-01 0.8363178921 6 43071852 43071922 71 + 2.119 2.138 0.064
ENSG00000137171 E039 211.7429640 0.0055443071 7.941451e-01 0.8684553966 6 43072147 43072255 109 + 2.237 2.255 0.059
ENSG00000137171 E040 6.0181438 0.0132830088 7.606534e-01 0.8446692243 6 43072741 43072823 83 + 0.810 0.768 -0.165
ENSG00000137171 E041 165.6501028 0.0091087208 5.489182e-01 0.6787274003 6 43072824 43072887 64 + 2.114 2.155 0.136
ENSG00000137171 E042 165.6417494 0.0028053041 8.773735e-02 0.1717051935 6 43072888 43072964 77 + 2.088 2.161 0.243
ENSG00000137171 E043 147.1546713 0.0031706763 9.974905e-04 0.0039494970 6 43073223 43073300 78 + 1.976 2.126 0.500
ENSG00000137171 E044 102.5240012 0.0036865267 1.543498e-03 0.0057766328 6 43073301 43073338 38 + 1.808 1.974 0.561
ENSG00000137171 E045 1.4715943 0.0092970498 6.425183e-01 0.7552844686 6 43073339 43073647 309 + 0.421 0.335 -0.488
ENSG00000137171 E046 2.3905985 0.0076196522 6.763191e-01 0.7813218619 6 43073684 43073901 218 + 0.421 0.497 0.387
ENSG00000137171 E047 117.3320704 0.0027761532 1.606178e-04 0.0007946871 6 43073902 43073965 64 + 1.853 2.034 0.609
ENSG00000137171 E048 217.5541764 0.0024774515 2.083221e-05 0.0001286906 6 43074622 43075206 585 + 2.134 2.296 0.541