ENSG00000136950

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000259477 ENSG00000136950 HEK293_OSMI2_2hA HEK293_TMG_2hB ARPC5L protein_coding protein_coding 56.39076 75.20641 49.99295 6.198781 0.53429 -0.5890342 48.078660 65.792515 41.133015 5.4322429 0.5612470 -0.6774952 0.84986250 0.87486667 0.8227333 -0.05213333 2.378653e-04 5.864538e-05 FALSE  
ENST00000465124 ENSG00000136950 HEK293_OSMI2_2hA HEK293_TMG_2hB ARPC5L protein_coding processed_transcript 56.39076 75.20641 49.99295 6.198781 0.53429 -0.5890342 3.493304 2.964762 3.661801 0.1126833 0.1666196 0.3037135 0.06607917 0.03973333 0.0732000 0.03346667 5.864538e-05 5.864538e-05 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000136950 E001 0.8815421 0.0274256334 9.494257e-01 9.723372e-01 9 124862070 124862124 55 + 0.252 0.257 0.036
ENSG00000136950 E002 1.2846917 0.1177111161 6.044485e-01 7.249961e-01 9 124862125 124862129 5 + 0.252 0.374 0.796
ENSG00000136950 E003 13.2550979 0.0014378273 3.367225e-01 4.805932e-01 9 124862130 124862373 244 + 1.170 1.117 -0.190
ENSG00000136950 E004 6.6428732 0.0024658802 5.124200e-02 1.113629e-01 9 124862374 124862398 25 + 0.985 0.786 -0.764
ENSG00000136950 E005 0.3634088 0.4138830548 4.662710e-01   9 124863243 124863254 12 + 0.001 0.188 8.817
ENSG00000136950 E006 0.6902858 0.5325837700 7.910523e-01 8.663623e-01 9 124863255 124863297 43 + 0.144 0.259 1.053
ENSG00000136950 E007 12.4709380 0.0014017933 1.753798e-02 4.612300e-02 9 124863988 124864107 120 + 1.224 1.039 -0.665
ENSG00000136950 E008 0.8062000 0.1152843268 9.513276e-01 9.735445e-01 9 124868189 124868189 1 + 0.252 0.256 0.028
ENSG00000136950 E009 3.1280015 0.0051229652 8.933357e-01 9.358349e-01 9 124868190 124868427 238 + 0.575 0.626 0.225
ENSG00000136950 E010 4.4390966 0.0094841153 8.986732e-01 9.393830e-01 9 124868428 124868453 26 + 0.694 0.745 0.210
ENSG00000136950 E011 8.3441413 0.0021314020 6.830324e-01 7.863490e-01 9 124868454 124868670 217 + 0.967 0.957 -0.039
ENSG00000136950 E012 81.8305347 0.0195352902 2.841195e-02 6.884124e-02 9 124868671 124869204 534 + 1.983 1.845 -0.462
ENSG00000136950 E013 107.7701820 0.0249938256 1.937074e-01 3.184367e-01 9 124869205 124869216 12 + 2.063 1.990 -0.247
ENSG00000136950 E014 417.7645924 0.0075291903 1.445360e-01 2.541953e-01 9 124869217 124869439 223 + 2.624 2.593 -0.103
ENSG00000136950 E015 11.9496314 0.0044610347 1.396279e-02 3.813793e-02 9 124869440 124869490 51 + 1.214 1.006 -0.749
ENSG00000136950 E016 17.0323369 0.0107771034 2.014292e-01 3.279717e-01 9 124869491 124869557 67 + 1.291 1.205 -0.300
ENSG00000136950 E017 104.3423192 0.0003030339 7.179802e-13 1.802534e-11 9 124872589 124873691 1103 + 2.129 1.920 -0.700
ENSG00000136950 E018 196.0696314 0.0043640736 1.652220e-02 4.389030e-02 9 124873692 124873693 2 + 2.317 2.256 -0.205
ENSG00000136950 E019 395.2749816 0.0002151593 3.748508e-03 1.243532e-02 9 124873694 124873764 71 + 2.593 2.579 -0.047
ENSG00000136950 E020 733.6680289 0.0002301184 5.294374e-01 6.622101e-01 9 124874975 124875151 177 + 2.822 2.870 0.157
ENSG00000136950 E021 1548.0357445 0.0047571806 1.513460e-04 7.530809e-04 9 124876878 124877733 856 + 3.094 3.212 0.393