ENSG00000136827

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000351698 ENSG00000136827 HEK293_OSMI2_2hA HEK293_TMG_2hB TOR1A protein_coding protein_coding 34.04431 31.07822 40.93982 3.808791 0.3306601 0.3974891 26.763526 25.505084 32.939387 3.0222061 0.3825492 0.3689012 0.7788417 0.8213667 0.80483333 -0.01653333 8.003210e-01 7.519557e-07 FALSE  
ENST00000474192 ENSG00000136827 HEK293_OSMI2_2hA HEK293_TMG_2hB TOR1A protein_coding processed_transcript 34.04431 31.07822 40.93982 3.808791 0.3306601 0.3974891 3.251859 2.905533 2.516004 0.6179512 0.3817739 -0.2069027 0.1009875 0.0915000 0.06140000 -0.03010000 2.328997e-01 7.519557e-07 TRUE  
MSTRG.33510.1 ENSG00000136827 HEK293_OSMI2_2hA HEK293_TMG_2hB TOR1A protein_coding   34.04431 31.07822 40.93982 3.808791 0.3306601 0.3974891 2.282225 1.223503 4.087152 0.2445068 0.2619549 1.7318602 0.0647125 0.0386000 0.09976667 0.06116667 7.519557e-07 7.519557e-07 TRUE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000136827 E001 3.891325 0.0044600791 8.149256e-03 2.415968e-02 9 129807859 129808034 176 - 0.445 0.848 1.760
ENSG00000136827 E002 35.415049 0.0006215679 1.858229e-07 1.791349e-06 9 129812675 129812941 267 - 1.692 1.395 -1.018
ENSG00000136827 E003 14.881029 0.0153127533 1.647400e-01 2.813517e-01 9 129812942 129812964 23 - 1.269 1.146 -0.435
ENSG00000136827 E004 405.364273 0.0054034747 2.689543e-01 4.074218e-01 9 129812965 129813607 643 - 2.584 2.638 0.180
ENSG00000136827 E005 867.133575 0.0008413169 6.443882e-06 4.474446e-05 9 129813608 129814222 615 - 2.904 2.978 0.246
ENSG00000136827 E006 13.275718 0.0028782544 6.750583e-01 7.804459e-01 9 129818178 129818297 120 - 1.167 1.140 -0.097
ENSG00000136827 E007 7.653969 0.0024193843 1.359375e-01 2.423990e-01 9 129818323 129818352 30 - 0.842 1.025 0.689
ENSG00000136827 E008 22.447137 0.0056964380 9.754720e-02 1.867461e-01 9 129818353 129818519 167 - 1.302 1.450 0.513
ENSG00000136827 E009 408.826400 0.0013210133 9.318668e-01 9.610723e-01 9 129818520 129818647 128 - 2.611 2.620 0.031
ENSG00000136827 E010 344.887582 0.0003031100 2.688543e-01 4.073258e-01 9 129818745 129818920 176 - 2.548 2.539 -0.029
ENSG00000136827 E011 10.330645 0.0029281510 1.071824e-02 3.049635e-02 9 129821376 129822580 1205 - 0.895 1.172 1.018
ENSG00000136827 E012 460.273502 0.0002995513 1.395120e-06 1.122463e-05 9 129822581 129822846 266 - 2.701 2.632 -0.228
ENSG00000136827 E013 1.112437 0.1865061130 1.038951e-01 1.964451e-01 9 129823306 129823593 288 - 0.113 0.448 2.597
ENSG00000136827 E014 240.438582 0.0040846964 1.499665e-03 5.634584e-03 9 129823908 129824244 337 - 2.438 2.325 -0.374