ENSG00000136824

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000374787 ENSG00000136824 HEK293_OSMI2_2hA HEK293_TMG_2hB SMC2 protein_coding protein_coding 21.32196 3.428449 38.34667 0.1794656 0.8719814 3.479647 1.2976387 0.38107651 1.9255505 0.17239960 0.27187967 2.30722120 0.05781250 0.110633333 0.05000000 -0.060633333 4.102014e-01 3.227624e-22 FALSE TRUE
ENST00000374793 ENSG00000136824 HEK293_OSMI2_2hA HEK293_TMG_2hB SMC2 protein_coding protein_coding 21.32196 3.428449 38.34667 0.1794656 0.8719814 3.479647 1.2891363 0.31590480 1.0136643 0.16447154 0.82784959 1.65122021 0.16872917 0.093266667 0.02640000 -0.066866667 6.491079e-01 3.227624e-22 FALSE TRUE
ENST00000493955 ENSG00000136824 HEK293_OSMI2_2hA HEK293_TMG_2hB SMC2 protein_coding nonsense_mediated_decay 21.32196 3.428449 38.34667 0.1794656 0.8719814 3.479647 0.3770926 0.51536681 0.4872016 0.51536681 0.07589919 -0.07949427 0.05016667 0.141133333 0.01263333 -0.128500000 9.858587e-01 3.227624e-22 TRUE TRUE
MSTRG.33177.1 ENSG00000136824 HEK293_OSMI2_2hA HEK293_TMG_2hB SMC2 protein_coding   21.32196 3.428449 38.34667 0.1794656 0.8719814 3.479647 0.9055589 0.00000000 2.8178837 0.00000000 0.83351651 8.14357898 0.02544583 0.000000000 0.07263333 0.072633333 1.329704e-04 3.227624e-22 FALSE TRUE
MSTRG.33177.2 ENSG00000136824 HEK293_OSMI2_2hA HEK293_TMG_2hB SMC2 protein_coding   21.32196 3.428449 38.34667 0.1794656 0.8719814 3.479647 5.8201139 1.09921744 12.8847903 0.59531724 0.40924238 3.53917419 0.22881250 0.339066667 0.33630000 -0.002766667 9.280080e-01 3.227624e-22 FALSE TRUE
MSTRG.33177.4 ENSG00000136824 HEK293_OSMI2_2hA HEK293_TMG_2hB SMC2 protein_coding   21.32196 3.428449 38.34667 0.1794656 0.8719814 3.479647 7.7339318 0.02983903 15.8681067 0.02983903 0.77562794 8.63864076 0.21810000 0.008166667 0.41410000 0.405933333 3.227624e-22 3.227624e-22 FALSE TRUE
MSTRG.33177.5 ENSG00000136824 HEK293_OSMI2_2hA HEK293_TMG_2hB SMC2 protein_coding   21.32196 3.428449 38.34667 0.1794656 0.8719814 3.479647 2.4827649 0.92755650 2.6563063 0.42688582 0.48119283 1.50786498 0.17716667 0.262866667 0.06990000 -0.192966667 1.414652e-01 3.227624e-22 FALSE TRUE
MSTRG.33177.7 ENSG00000136824 HEK293_OSMI2_2hA HEK293_TMG_2hB SMC2 protein_coding   21.32196 3.428449 38.34667 0.1794656 0.8719814 3.479647 0.9689991 0.07372918 0.0000000 0.07372918 0.00000000 -3.06573054 0.05556667 0.020700000 0.00000000 -0.020700000 2.895346e-01 3.227624e-22 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000136824 E001 2.0618276 0.0067593646 2.248886e-01 3.562942e-01 9 104094177 104094242 66 + 0.365 0.000 -10.450
ENSG00000136824 E002 1.4706414 0.0091818047 3.899563e-01 5.339883e-01 9 104094243 104094259 17 + 0.288 0.000 -9.955
ENSG00000136824 E003 2.2070002 0.0068765919 1.983134e-01 3.241102e-01 9 104094260 104094285 26 + 0.382 0.000 -10.540
ENSG00000136824 E004 2.0724367 0.0068038105 2.247491e-01 3.561317e-01 9 104094286 104094295 10 + 0.365 0.000 -10.441
ENSG00000136824 E005 5.9026379 0.0028240235 1.009996e-01 1.919876e-01 9 104094296 104094308 13 + 0.669 0.328 -1.700
ENSG00000136824 E006 107.4796197 0.0037385257 4.569283e-06 3.282534e-05 9 104094309 104094448 140 + 1.818 1.529 -0.982
ENSG00000136824 E007 68.9012217 0.0011560606 1.017613e-04 5.300190e-04 9 104094449 104094477 29 + 1.629 1.351 -0.954
ENSG00000136824 E008 22.4815290 0.0009057516 5.386289e-01 6.700438e-01 9 104094478 104094486 9 + 1.142 1.127 -0.053
ENSG00000136824 E009 8.1094452 0.0026709740 1.580617e-01 2.725383e-01 9 104094564 104094838 275 + 0.726 1.001 1.061
ENSG00000136824 E010 5.8033344 0.0137986678 1.155252e-01 2.136002e-01 9 104095299 104095323 25 + 0.660 0.328 -1.662
ENSG00000136824 E011 181.8701226 0.0001974994 2.508419e-09 3.454332e-08 9 104095324 104095552 229 + 2.042 1.792 -0.841
ENSG00000136824 E012 128.0873375 0.0011559563 1.397243e-06 1.123911e-05 9 104096148 104096297 150 + 1.891 1.642 -0.840
ENSG00000136824 E013 112.4505376 0.0283135881 4.180325e-02 9.436852e-02 9 104098446 104098568 123 + 1.827 1.695 -0.447
ENSG00000136824 E014 81.8793270 0.0003634398 1.935236e-03 7.025479e-03 9 104099644 104099682 39 + 1.694 1.528 -0.568
ENSG00000136824 E015 121.0478068 0.0008031230 2.101501e-06 1.627118e-05 9 104100093 104100203 111 + 1.867 1.619 -0.838
ENSG00000136824 E016 80.9510218 0.0006529189 8.108203e-05 4.331119e-04 9 104100389 104100433 45 + 1.696 1.448 -0.846
ENSG00000136824 E017 180.0873695 0.0019424577 1.301235e-05 8.415207e-05 9 104101960 104102193 234 + 2.034 1.858 -0.590
ENSG00000136824 E018 153.8091319 0.0008540423 1.732324e-03 6.384770e-03 9 104102424 104102573 150 + 1.962 1.858 -0.350
ENSG00000136824 E019 177.1181316 0.0002825052 3.197669e-03 1.084943e-02 9 104111581 104111814 234 + 2.020 1.944 -0.258
ENSG00000136824 E020 133.9488517 0.0003012575 2.811619e-02 6.824332e-02 9 104113316 104113475 160 + 1.898 1.844 -0.184
ENSG00000136824 E021 125.9628770 0.0002347400 2.778003e-03 9.607936e-03 9 104113964 104114081 118 + 1.876 1.767 -0.366
ENSG00000136824 E022 154.4786456 0.0002194264 4.137912e-03 1.352861e-02 9 104114691 104114829 139 + 1.960 1.878 -0.278
ENSG00000136824 E023 157.0028607 0.0044253301 4.172467e-01 5.606070e-01 9 104116200 104116319 120 + 1.958 1.978 0.067
ENSG00000136824 E024 217.8673711 0.0004083063 1.390012e-01 2.467196e-01 9 104118171 104118375 205 + 2.101 2.105 0.013
ENSG00000136824 E025 178.5374820 0.0002366201 3.088279e-01 4.510923e-01 9 104120027 104120162 136 + 2.014 2.034 0.065
ENSG00000136824 E026 176.0889802 0.0002814609 3.910340e-01 5.349795e-01 9 104123108 104123232 125 + 2.007 2.034 0.089
ENSG00000136824 E027 208.0103128 0.0001982485 2.431306e-01 3.778567e-01 9 104124912 104125105 194 + 2.080 2.097 0.056
ENSG00000136824 E028 173.5386365 0.0002446071 9.668580e-01 9.832272e-01 9 104126641 104126784 144 + 1.997 2.065 0.226
ENSG00000136824 E029 168.0325652 0.0003721610 5.403131e-01 6.714731e-01 9 104127286 104127406 121 + 1.980 2.073 0.312
ENSG00000136824 E030 143.1505939 0.0005716559 7.466396e-01 8.343303e-01 9 104127407 104127480 74 + 1.916 1.965 0.166
ENSG00000136824 E031 220.2695323 0.0002291467 2.651415e-01 4.031677e-01 9 104129645 104129845 201 + 2.096 2.204 0.363
ENSG00000136824 E032 159.4296691 0.0002295604 4.292658e-02 9.640695e-02 9 104132009 104132125 117 + 1.950 2.105 0.518
ENSG00000136824 E033 164.4213031 0.0002430419 5.990633e-04 2.529831e-03 9 104134415 104134575 161 + 1.956 2.166 0.702
ENSG00000136824 E034 6.4196253 0.0025595133 6.577177e-01 7.670262e-01 9 104135868 104136060 193 + 0.677 0.642 -0.152
ENSG00000136824 E035 185.8595773 0.0002374504 3.349916e-04 1.520403e-03 9 104138018 104138165 148 + 2.009 2.216 0.694
ENSG00000136824 E036 673.8547274 0.0165220103 3.015125e-09 4.089100e-08 9 104139139 104141400 2262 + 2.535 2.931 1.317
ENSG00000136824 E037 10.5408879 0.0433478186 4.729168e-01 6.119509e-01 9 104141401 104141520 120 + 0.828 1.003 0.661
ENSG00000136824 E038 0.8148214 0.0187040738 4.179421e-01 5.612546e-01 9 104149047 104149180 134 + 0.139 0.328 1.587