ENSG00000136819

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000372447 ENSG00000136819 HEK293_OSMI2_2hA HEK293_TMG_2hB C9orf78 protein_coding protein_coding 50.97384 41.76493 64.16358 4.08061 0.2598105 0.619342 38.867215 34.716424 45.699503 4.312255 1.402746 0.3964602 0.7822750 0.82720000 0.7121000 -0.11510000 0.02299564 0.02299564 FALSE TRUE
ENST00000461539 ENSG00000136819 HEK293_OSMI2_2hA HEK293_TMG_2hB C9orf78 protein_coding processed_transcript 50.97384 41.76493 64.16358 4.08061 0.2598105 0.619342 3.151007 3.370576 1.150429 1.048863 1.150429 -1.5426112 0.0587375 0.08233333 0.0180000 -0.06433333 0.11258649 0.02299564 FALSE FALSE
ENST00000492991 ENSG00000136819 HEK293_OSMI2_2hA HEK293_TMG_2hB C9orf78 protein_coding processed_transcript 50.97384 41.76493 64.16358 4.08061 0.2598105 0.619342 5.480700 1.269753 12.307790 1.269753 1.196542 3.2668061 0.0921875 0.03440000 0.1919333 0.15753333 0.07511209 0.02299564 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000136819 E001 1.9425868 0.1166621235 7.000590e-01 7.996266e-01 9 129827290 129827314 25 - 0.439 0.501 0.312
ENSG00000136819 E002 530.9526243 0.0028207483 6.776487e-05 3.693708e-04 9 129827315 129827967 653 - 2.668 2.784 0.388
ENSG00000136819 E003 508.8759153 0.0018178652 1.069831e-03 4.200545e-03 9 129827968 129828252 285 - 2.668 2.752 0.281
ENSG00000136819 E004 0.5901540 0.0195247686 4.170484e-01 5.604319e-01 9 129828428 129828441 14 - 0.273 0.134 -1.276
ENSG00000136819 E005 10.6149085 0.0016620406 1.334322e-01 2.389053e-01 9 129828442 129829204 763 - 1.132 0.987 -0.531
ENSG00000136819 E006 224.4272238 0.0004307455 1.641042e-03 6.092546e-03 9 129829205 129829235 31 - 2.316 2.401 0.285
ENSG00000136819 E007 249.0349315 0.0003214648 6.589355e-02 1.365310e-01 9 129829236 129829278 43 - 2.379 2.428 0.164
ENSG00000136819 E008 197.1653341 0.0003494024 3.479809e-02 8.125818e-02 9 129829279 129829303 25 - 2.271 2.333 0.205
ENSG00000136819 E009 349.6428888 0.0001673459 1.791793e-03 6.573263e-03 9 129829405 129829541 137 - 2.517 2.583 0.220
ENSG00000136819 E010 3.0322128 0.0053341721 2.388790e-01 3.728182e-01 9 129829542 129830685 1144 - 0.681 0.502 -0.805
ENSG00000136819 E011 293.9134968 0.0001453679 1.126248e-01 2.093819e-01 9 129830871 129830934 64 - 2.456 2.495 0.131
ENSG00000136819 E012 247.3669265 0.0003470512 5.987192e-03 1.857578e-02 9 129830935 129830961 27 - 2.366 2.437 0.237
ENSG00000136819 E013 486.7394431 0.0001409330 3.399879e-01 4.839304e-01 9 129830962 129831068 107 - 2.683 2.705 0.072
ENSG00000136819 E014 2.9215085 0.0065789451 6.094008e-01 7.289167e-01 9 129831410 129831732 323 - 0.625 0.549 -0.341
ENSG00000136819 E015 489.0818095 0.0010639991 5.141119e-01 6.488589e-01 9 129831896 129831973 78 - 2.700 2.687 -0.042
ENSG00000136819 E016 463.5312577 0.0003230759 1.057338e-02 3.014577e-02 9 129833447 129833517 71 - 2.690 2.649 -0.135
ENSG00000136819 E017 3.9221739 0.0071578822 4.733219e-01 6.122949e-01 9 129833518 129833657 140 - 0.731 0.630 -0.425
ENSG00000136819 E018 388.4576970 0.0001521750 2.020891e-08 2.354417e-07 9 129833658 129833709 52 - 2.639 2.544 -0.318
ENSG00000136819 E019 10.5126154 0.0015983644 4.346136e-03 1.411172e-02 9 129833710 129834547 838 - 1.185 0.897 -1.057
ENSG00000136819 E020 231.6034394 0.0002313360 1.422224e-08 1.704213e-07 9 129834707 129834711 5 - 2.429 2.301 -0.429
ENSG00000136819 E021 354.4188532 0.0013710694 1.349821e-07 1.337376e-06 9 129834712 129834766 55 - 2.613 2.481 -0.439
ENSG00000136819 E022 311.5854436 0.0022458525 1.393682e-06 1.121427e-05 9 129835139 129835321 183 - 2.561 2.417 -0.482
ENSG00000136819 E023 0.2214452 0.0462386785 3.963275e-01   9 129835788 129835863 76 - 0.000 0.135 9.417