ENSG00000136754

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000376139 ENSG00000136754 HEK293_OSMI2_2hA HEK293_TMG_2hB ABI1 protein_coding protein_coding 15.70123 8.276214 22.79718 0.8609042 0.2416326 1.460703 1.415063 1.224253 1.846749 0.6159054 0.1477932 0.5891400 0.1095000 0.1407000 0.08106667 -0.05963333 0.998482852 0.005847245 FALSE TRUE
ENST00000376160 ENSG00000136754 HEK293_OSMI2_2hA HEK293_TMG_2hB ABI1 protein_coding protein_coding 15.70123 8.276214 22.79718 0.8609042 0.2416326 1.460703 2.684678 1.383159 3.925660 0.6915837 0.2191789 1.4982460 0.1632167 0.1646333 0.17223333 0.00760000 0.876378162 0.005847245 FALSE TRUE
MSTRG.3685.13 ENSG00000136754 HEK293_OSMI2_2hA HEK293_TMG_2hB ABI1 protein_coding   15.70123 8.276214 22.79718 0.8609042 0.2416326 1.460703 3.621209 2.049065 3.882040 0.2089405 0.3103833 0.9185371 0.2478333 0.2550000 0.17016667 -0.08483333 0.309936179 0.005847245 FALSE TRUE
MSTRG.3685.14 ENSG00000136754 HEK293_OSMI2_2hA HEK293_TMG_2hB ABI1 protein_coding   15.70123 8.276214 22.79718 0.8609042 0.2416326 1.460703 7.288605 3.092445 12.045498 0.2609505 0.3632808 1.9582135 0.4288042 0.3770000 0.52833333 0.15133333 0.005847245 0.005847245 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000136754 E001 0.2924217 0.0272667614 5.570962e-01   10 26746593 26746595 3 - 0.153 0.000 -8.976
ENSG00000136754 E002 4.3009847 0.0888623877 4.176124e-01 5.609391e-01 10 26746596 26746894 299 - 0.641 0.786 0.597
ENSG00000136754 E003 99.9436453 0.0092202773 7.443100e-04 3.058528e-03 10 26746895 26747863 969 - 1.901 2.117 0.725
ENSG00000136754 E004 84.8630042 0.0003704715 7.683746e-13 1.921920e-11 10 26747864 26748175 312 - 1.792 2.085 0.986
ENSG00000136754 E005 286.1801096 0.0047320402 8.760002e-06 5.898756e-05 10 26748176 26748745 570 - 2.373 2.537 0.545
ENSG00000136754 E006 142.5024465 0.0002540996 7.167915e-01 8.123497e-01 10 26751598 26751783 186 - 2.126 2.157 0.103
ENSG00000136754 E007 90.0867258 0.0037438785 1.290076e-01 2.327894e-01 10 26755655 26755741 87 - 1.956 1.906 -0.169
ENSG00000136754 E008 155.1142416 0.0078190178 1.430265e-01 2.521468e-01 10 26759062 26759235 174 - 2.189 2.141 -0.158
ENSG00000136754 E009 43.0641020 0.0050584995 2.125729e-01 3.415842e-01 10 26759236 26759238 3 - 1.645 1.584 -0.207
ENSG00000136754 E010 1.1845624 0.0107461385 3.218246e-02 7.620706e-02 10 26763880 26763960 81 - 0.430 0.000 -10.975
ENSG00000136754 E011 130.3929147 0.0007790412 7.495228e-02 1.514595e-01 10 26765218 26765318 101 - 2.113 2.072 -0.139
ENSG00000136754 E012 133.4219152 0.0002737589 6.220273e-03 1.919435e-02 10 26768852 26768992 141 - 2.131 2.061 -0.233
ENSG00000136754 E013 106.6596953 0.0077299104 1.374728e-01 2.445657e-01 10 26770245 26770345 101 - 2.033 1.958 -0.253
ENSG00000136754 E014 3.7372498 0.0043899146 5.454953e-01 6.758251e-01 10 26771075 26771089 15 - 0.682 0.603 -0.342
ENSG00000136754 E015 0.0000000       10 26776626 26777064 439 -      
ENSG00000136754 E016 141.6678746 0.0009461909 3.508835e-03 1.175197e-02 10 26777065 26777241 177 - 2.160 2.079 -0.272
ENSG00000136754 E017 143.5149021 0.0002437747 3.695860e-04 1.656422e-03 10 26823138 26823305 168 - 2.168 2.076 -0.309
ENSG00000136754 E018 0.5985731 0.0212344651 8.299389e-01 8.932324e-01 10 26839733 26839783 51 - 0.213 0.176 -0.344
ENSG00000136754 E019 113.6404063 0.0002826028 4.496317e-05 2.561769e-04 10 26860747 26861087 341 - 2.079 1.952 -0.424