ENSG00000136527

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000259043 ENSG00000136527 HEK293_OSMI2_2hA HEK293_TMG_2hB TRA2B protein_coding protein_coding 186.2608 121.6352 248.3816 25.47174 4.479581 1.029937 32.317600 26.5642406 36.573767 5.7405565 3.2904258 0.4611752 0.18082500 0.217800000 0.1469333 -0.07086667 5.696761e-03 1.276304e-11 FALSE FALSE
ENST00000382191 ENSG00000136527 HEK293_OSMI2_2hA HEK293_TMG_2hB TRA2B protein_coding protein_coding 186.2608 121.6352 248.3816 25.47174 4.479581 1.029937 51.254498 24.8177856 75.568687 5.7917479 2.2696487 1.6060239 0.25371667 0.202233333 0.3041667 0.10193333 1.276304e-11 1.276304e-11   TRUE
ENST00000414862 ENSG00000136527 HEK293_OSMI2_2hA HEK293_TMG_2hB TRA2B protein_coding protein_coding 186.2608 121.6352 248.3816 25.47174 4.479581 1.029937 9.689225 10.9374139 9.988407 3.7818753 0.3065887 -0.1308199 0.05995833 0.085633333 0.0402000 -0.04543333 9.183346e-04 1.276304e-11 FALSE TRUE
ENST00000456380 ENSG00000136527 HEK293_OSMI2_2hA HEK293_TMG_2hB TRA2B protein_coding nonsense_mediated_decay 186.2608 121.6352 248.3816 25.47174 4.479581 1.029937 45.229504 30.8717859 58.694386 4.9695070 1.1048627 0.9267121 0.24969167 0.258433333 0.2363000 -0.02213333 5.530409e-01 1.276304e-11 FALSE TRUE
ENST00000485530 ENSG00000136527 HEK293_OSMI2_2hA HEK293_TMG_2hB TRA2B protein_coding retained_intron 186.2608 121.6352 248.3816 25.47174 4.479581 1.029937 8.005775 0.6930308 16.075448 0.6930308 4.9764234 4.5160244 0.04448333 0.007566667 0.0651000 0.05753333 6.715134e-02 1.276304e-11 FALSE FALSE
MSTRG.24221.3 ENSG00000136527 HEK293_OSMI2_2hA HEK293_TMG_2hB TRA2B protein_coding   186.2608 121.6352 248.3816 25.47174 4.479581 1.029937 30.498987 24.7239974 37.322743 5.5743953 1.7517513 0.5939461 0.16032083 0.201966667 0.1501667 -0.05180000 3.121429e-04 1.276304e-11 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000136527 E001 35.743329 2.979507e-03 5.457835e-02 1.172515e-01 3 185914558 185916743 2186 - 1.615 1.480 -0.463
ENSG00000136527 E002 9.408321 4.251188e-02 5.785093e-02 1.229453e-01 3 185916744 185916748 5 - 0.886 1.164 1.021
ENSG00000136527 E003 839.068587 1.319244e-04 1.939423e-06 1.512942e-05 3 185916749 185917154 406 - 2.903 2.960 0.189
ENSG00000136527 E004 765.894676 3.941737e-04 1.524138e-02 4.103334e-02 3 185917155 185917327 173 - 2.874 2.905 0.102
ENSG00000136527 E005 299.228927 8.433822e-04 1.795121e-02 4.702893e-02 3 185917328 185917328 1 - 2.459 2.510 0.170
ENSG00000136527 E006 294.514357 9.664871e-04 2.093679e-02 5.345366e-02 3 185917329 185917329 1 - 2.452 2.502 0.170
ENSG00000136527 E007 1975.588628 3.901284e-04 8.775915e-25 9.403681e-23 3 185917330 185917714 385 - 3.248 3.355 0.355
ENSG00000136527 E008 706.216891 5.833107e-04 2.838879e-06 2.136399e-05 3 185917715 185917725 11 - 2.819 2.893 0.246
ENSG00000136527 E009 1005.707676 1.604508e-04 3.776717e-10 6.049101e-09 3 185918365 185918435 71 - 2.975 3.047 0.239
ENSG00000136527 E010 560.551697 1.476851e-04 5.792555e-04 2.457247e-03 3 185918436 185918438 3 - 2.732 2.782 0.165
ENSG00000136527 E011 804.495452 1.303594e-04 8.380014e-02 1.655010e-01 3 185919437 185919496 60 - 2.902 2.921 0.061
ENSG00000136527 E012 1167.094693 1.142287e-04 4.471948e-01 5.886093e-01 3 185921104 185921187 84 - 3.077 3.064 -0.045
ENSG00000136527 E013 1031.506939 8.365413e-05 6.927921e-01 7.939352e-01 3 185922011 185922042 32 - 3.022 3.013 -0.031
ENSG00000136527 E014 1316.325630 2.873426e-04 4.666547e-02 1.032314e-01 3 185922043 185922126 84 - 3.136 3.103 -0.108
ENSG00000136527 E015 28.736793 6.155408e-03 3.187845e-03 1.081939e-02 3 185922127 185923795 1669 - 1.560 1.316 -0.840
ENSG00000136527 E016 1665.763196 8.196364e-05 2.911496e-09 3.961320e-08 3 185923796 185923984 189 - 3.250 3.188 -0.205
ENSG00000136527 E017 5.633227 1.449482e-02 8.075925e-01 8.777486e-01 3 185923985 185924008 24 - 0.841 0.801 -0.155
ENSG00000136527 E018 8.952116 6.611602e-02 6.916740e-02 1.419917e-01 3 185924009 185924804 796 - 1.111 0.772 -1.277
ENSG00000136527 E019 4.738017 4.814733e-02 8.248251e-02 1.634252e-01 3 185924805 185925084 280 - 0.856 0.542 -1.316
ENSG00000136527 E020 4.528310 4.386778e-01 5.264719e-01 6.597030e-01 3 185925085 185925229 145 - 0.772 0.664 -0.444
ENSG00000136527 E021 1.420769 2.776840e-01 2.104810e-01 3.391019e-01 3 185925230 185925272 43 - 0.488 0.152 -2.306
ENSG00000136527 E022 6.081365 1.751438e-01 3.896887e-01 5.337557e-01 3 185925273 185925463 191 - 0.916 0.717 -0.781
ENSG00000136527 E023 1501.359978 6.066438e-04 3.502608e-05 2.047641e-04 3 185925464 185925626 163 - 3.207 3.134 -0.245
ENSG00000136527 E024 1048.713014 3.125840e-03 4.495783e-02 1.001214e-01 3 185926601 185926726 126 - 3.051 2.973 -0.260
ENSG00000136527 E025 410.643156 2.328776e-03 4.178878e-03 1.364647e-02 3 185926727 185926734 8 - 2.654 2.558 -0.322
ENSG00000136527 E026 153.279038 1.465884e-02 4.648864e-13 1.203654e-11 3 185926735 185929073 2339 - 2.345 1.801 -1.824
ENSG00000136527 E027 7.211967 7.246852e-02 8.361628e-03 2.469404e-02 3 185931515 185931576 62 - 1.062 0.544 -2.078
ENSG00000136527 E028 61.229911 2.460774e-02 1.357616e-05 8.745198e-05 3 185931577 185931852 276 - 1.929 1.483 -1.512
ENSG00000136527 E029 7.951504 2.311542e-02 1.075863e-06 8.868254e-06 3 185934429 185934505 77 - 1.143 0.349 -3.389
ENSG00000136527 E030 48.120446 1.213017e-02 4.735504e-22 3.798167e-20 3 185934506 185936237 1732 - 1.894 0.966 -3.231
ENSG00000136527 E031 30.362230 6.470842e-03 8.512101e-28 1.186602e-25 3 185936238 185937420 1183 - 1.718 0.485 -4.642
ENSG00000136527 E032 839.452271 2.917384e-03 2.538622e-02 6.267225e-02 3 185937825 185938103 279 - 2.958 2.873 -0.281