ENSG00000136522

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000392659 ENSG00000136522 HEK293_OSMI2_2hA HEK293_TMG_2hB MRPL47 protein_coding protein_coding 33.71813 20.50097 53.2152 2.694708 0.9073506 1.375714 10.15761 7.916232 15.42478 1.024738 0.2260107 0.9614782 0.3011292 0.3872667 0.2901667 -0.0971000 0.014622892 0.001692317 FALSE TRUE
ENST00000476781 ENSG00000136522 HEK293_OSMI2_2hA HEK293_TMG_2hB MRPL47 protein_coding protein_coding 33.71813 20.50097 53.2152 2.694708 0.9073506 1.375714 23.33942 12.314883 37.51817 1.786014 1.0814793 1.6063999 0.6925667 0.5988000 0.7047667 0.1059667 0.001692317 0.001692317 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000136522 E001 12.95617 0.0046553280 0.019462137 0.05033507 3 179588285 179588454 170 - 1.202 0.951 -0.911
ENSG00000136522 E002 85.86276 0.0003974559 0.916275468 0.95107601 3 179588455 179588830 376 - 1.922 1.929 0.024
ENSG00000136522 E003 219.06302 0.0021350073 0.052540607 0.11368336 3 179588831 179588995 165 - 2.301 2.366 0.214
ENSG00000136522 E004 315.28020 0.0001555280 0.015025509 0.04054493 3 179592644 179592739 96 - 2.464 2.515 0.169
ENSG00000136522 E005 350.72565 0.0001381523 0.015783415 0.04224587 3 179593765 179593895 131 - 2.511 2.559 0.158
ENSG00000136522 E006 243.80277 0.0001823027 0.124779791 0.22681978 3 179598675 179598771 97 - 2.358 2.396 0.126
ENSG00000136522 E007 250.35885 0.0002395705 0.396182569 0.53996978 3 179601730 179601790 61 - 2.387 2.369 -0.061
ENSG00000136522 E008 306.61162 0.0001547285 0.013705996 0.03755347 3 179602652 179602737 86 - 2.485 2.436 -0.165
ENSG00000136522 E009 263.52106 0.0002157688 0.007383111 0.02221406 3 179602738 179602797 60 - 2.422 2.362 -0.200
ENSG00000136522 E010 258.77635 0.0002412932 0.005937149 0.01844347 3 179604527 179604649 123 - 2.415 2.352 -0.210