ENSG00000136518

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000429709 ENSG00000136518 HEK293_OSMI2_2hA HEK293_TMG_2hB ACTL6A protein_coding protein_coding 38.04674 21.71047 55.58498 3.749509 1.658742 1.355899 27.935959 17.651378 37.268989 2.8465985 1.2250925 1.077765 0.7560542 0.8157667 0.6704000 -0.1453667 4.612137e-07 4.988014e-11 FALSE TRUE
ENST00000450518 ENSG00000136518 HEK293_OSMI2_2hA HEK293_TMG_2hB ACTL6A protein_coding protein_coding 38.04674 21.71047 55.58498 3.749509 1.658742 1.355899 5.908595 3.133833 9.113951 0.8070107 1.4259161 1.537134 0.1458708 0.1425667 0.1652667 0.0227000 8.437678e-01 4.988014e-11 FALSE TRUE
ENST00000468767 ENSG00000136518 HEK293_OSMI2_2hA HEK293_TMG_2hB ACTL6A protein_coding processed_transcript 38.04674 21.71047 55.58498 3.749509 1.658742 1.355899 1.585164 0.000000 3.722595 0.0000000 0.8661664 8.544035 0.0326125 0.0000000 0.0664000 0.0664000 4.988014e-11 4.988014e-11 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000136518 E001 0.2966881 0.0270578562 4.885918e-01   3 179562886 179562919 34 + 0.165 0.000 -11.740
ENSG00000136518 E002 0.2966881 0.0270578562 4.885918e-01   3 179562920 179562922 3 + 0.165 0.000 -11.735
ENSG00000136518 E003 0.2966881 0.0270578562 4.885918e-01   3 179562923 179562925 3 + 0.165 0.000 -11.735
ENSG00000136518 E004 0.8921407 0.0133041653 5.696817e-02 1.214040e-01 3 179562926 179562929 4 + 0.378 0.000 -13.320
ENSG00000136518 E005 50.8200340 0.0013092010 5.393314e-07 4.739966e-06 3 179562930 179562958 29 + 1.796 1.513 -0.961
ENSG00000136518 E006 66.4516829 0.0004178780 1.705112e-08 2.013692e-07 3 179562959 179562973 15 + 1.906 1.635 -0.915
ENSG00000136518 E007 157.0849553 0.0002678372 7.463148e-02 1.509465e-01 3 179562974 179563117 144 + 2.211 2.158 -0.176
ENSG00000136518 E008 0.2214452 0.0633914516 2.404349e-01   3 179563118 179563165 48 + 0.000 0.165 12.445
ENSG00000136518 E009 0.9317929 0.0648853472 1.000000e+00 1.000000e+00 3 179569815 179569823 9 + 0.284 0.282 -0.017
ENSG00000136518 E010 211.9357370 0.0011515866 7.144169e-03 2.159804e-02 3 179569824 179569900 77 + 2.350 2.267 -0.276
ENSG00000136518 E011 326.1273466 0.0001453559 3.035460e-05 1.803993e-04 3 179570067 179570241 175 + 2.538 2.453 -0.283
ENSG00000136518 E012 3.1086041 0.0048252880 1.371678e-03 5.213994e-03 3 179570242 179570391 150 + 0.750 0.164 -3.334
ENSG00000136518 E013 1.4759519 0.0165288755 8.876132e-02 1.732224e-01 3 179573234 179573368 135 + 0.488 0.164 -2.182
ENSG00000136518 E014 248.9831291 0.0002166417 7.316363e-02 1.485805e-01 3 179573369 179573469 101 + 2.407 2.366 -0.138
ENSG00000136518 E015 1.8069817 0.0076574638 1.941049e-01 3.189295e-01 3 179574353 179574369 17 + 0.520 0.283 -1.334
ENSG00000136518 E016 256.2129541 0.0003196882 6.275966e-02 1.313017e-01 3 179574370 179574467 98 + 2.420 2.376 -0.145
ENSG00000136518 E017 5.7207285 0.0028443340 4.465201e-03 1.444361e-02 3 179574468 179574501 34 + 0.936 0.517 -1.734
ENSG00000136518 E018 9.6300426 0.0020819232 1.406409e-01 2.488977e-01 3 179574502 179575009 508 + 1.082 0.920 -0.598
ENSG00000136518 E019 6.5122790 0.0084914460 1.074021e-01 2.017120e-01 3 179575010 179575402 393 + 0.936 0.710 -0.884
ENSG00000136518 E020 6.8136984 0.0136933761 1.904271e-01 3.142961e-01 3 179575403 179575504 102 + 0.936 0.748 -0.730
ENSG00000136518 E021 240.0020766 0.0001969398 1.019655e-01 1.934996e-01 3 179576217 179576311 95 + 2.388 2.349 -0.130
ENSG00000136518 E022 1.4112175 0.3828918737 6.522640e-01 7.629864e-01 3 179576376 179576619 244 + 0.419 0.284 -0.816
ENSG00000136518 E023 270.8774951 0.0001892311 7.332047e-01 8.244603e-01 3 179576620 179576726 107 + 2.430 2.423 -0.025
ENSG00000136518 E024 232.8846186 0.0002159397 9.729425e-01 9.870992e-01 3 179576824 179576913 90 + 2.363 2.365 0.005
ENSG00000136518 E025 209.3809539 0.0002226405 1.428271e-01 2.518890e-01 3 179580640 179580701 62 + 2.303 2.340 0.125
ENSG00000136518 E026 321.8749328 0.0001803805 5.620759e-01 6.897588e-01 3 179580894 179581008 115 + 2.499 2.511 0.040
ENSG00000136518 E027 197.3883814 0.0002013095 8.362414e-01 8.976035e-01 3 179581140 179581163 24 + 2.289 2.294 0.019
ENSG00000136518 E028 262.4198313 0.0001862073 8.981597e-01 9.390120e-01 3 179581164 179581220 57 + 2.412 2.415 0.010
ENSG00000136518 E029 288.8803075 0.0003170004 6.490850e-02 1.348842e-01 3 179583353 179583448 96 + 2.440 2.480 0.135
ENSG00000136518 E030 0.7718584 0.0162476258 7.654244e-01 8.481996e-01 3 179586488 179586545 58 + 0.229 0.282 0.403
ENSG00000136518 E031 246.6822888 0.0001868154 8.721844e-05 4.619698e-04 3 179586546 179586632 87 + 2.351 2.441 0.301
ENSG00000136518 E032 0.1482932 0.0411597534 2.439301e-01   3 179586633 179586708 76 + 0.000 0.164 12.435
ENSG00000136518 E033 337.7862383 0.0002922566 2.212521e-28 3.239222e-26 3 179587930 179588407 478 + 2.427 2.646 0.729