ENSG00000136451

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000258963 ENSG00000136451 HEK293_OSMI2_2hA HEK293_TMG_2hB VEZF1 protein_coding protein_coding 7.343696 3.570318 9.433863 0.3001533 0.1227514 1.39929 1.242104 1.495184 1.100141 0.25294769 0.2210223 -0.4391966 0.2214917 0.4131667 0.1162667 -0.2969000 2.663108e-06 1.439493e-15 FALSE TRUE
ENST00000581208 ENSG00000136451 HEK293_OSMI2_2hA HEK293_TMG_2hB VEZF1 protein_coding protein_coding 7.343696 3.570318 9.433863 0.3001533 0.1227514 1.39929 4.235534 2.049864 5.401028 0.07003358 0.3619342 1.3933534 0.5905625 0.5789333 0.5732333 -0.0057000 1.000000e+00 1.439493e-15 FALSE TRUE
ENST00000584396 ENSG00000136451 HEK293_OSMI2_2hA HEK293_TMG_2hB VEZF1 protein_coding protein_coding 7.343696 3.570318 9.433863 0.3001533 0.1227514 1.39929 1.508417 0.000000 2.489366 0.00000000 0.2871302 7.9654187 0.1462583 0.0000000 0.2633333 0.2633333 1.439493e-15 1.439493e-15 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000136451 E001 0.000000       17 57971547 57971551 5 -      
ENSG00000136451 E002 29.574219 0.0173406350 9.296208e-09 1.153106e-07 17 57971552 57971746 195 - 1.182 1.771 2.029
ENSG00000136451 E003 295.270929 0.0045259015 6.834633e-20 4.278918e-18 17 57971747 57973758 2012 - 2.317 2.632 1.050
ENSG00000136451 E004 166.081954 0.0003285610 1.585083e-01 2.731292e-01 17 57973759 57974900 1142 - 2.183 2.214 0.102
ENSG00000136451 E005 46.745674 0.0139487312 4.689891e-02 1.036461e-01 17 57979152 57979313 162 - 1.677 1.581 -0.325
ENSG00000136451 E006 55.103299 0.0051231269 8.730266e-04 3.519883e-03 17 57980603 57980786 184 - 1.760 1.620 -0.474
ENSG00000136451 E007 6.611947 0.0029794794 2.871961e-01 4.276687e-01 17 57980787 57980804 18 - 0.875 0.794 -0.317
ENSG00000136451 E008 36.071209 0.0042485170 6.831092e-05 3.720034e-04 17 57981873 57981936 64 - 1.600 1.379 -0.760
ENSG00000136451 E009 67.423541 0.0004041758 3.964339e-15 1.384902e-13 17 57982699 57982909 211 - 1.886 1.562 -1.099
ENSG00000136451 E010 51.725823 0.0019745474 9.140716e-09 1.135305e-07 17 57982910 57983026 117 - 1.762 1.488 -0.935
ENSG00000136451 E011 71.937342 0.0046513630 2.502184e-07 2.353564e-06 17 57983027 57983393 367 - 1.894 1.671 -0.754
ENSG00000136451 E012 3.585952 0.0042903381 8.622237e-04 3.481366e-03 17 57985254 57985465 212 - 0.754 0.182 -3.163
ENSG00000136451 E013 1.654422 0.0081435868 7.986515e-02 1.593520e-01 17 57985985 57986144 160 - 0.482 0.182 -1.961
ENSG00000136451 E014 17.673769 0.0191844697 1.124586e-01 2.091222e-01 17 57988079 57988269 191 - 1.280 1.162 -0.417