ENSG00000136450

Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000258962 ENSG00000136450 HEK293_OSMI2_2hA HEK293_TMG_2hB SRSF1 protein_coding protein_coding 214.1002 110.0328 302.2643 19.00435 7.203718 1.457793 15.04181 7.094758 22.58571 0.8379921 1.014977 1.6691917 0.06997083 0.06593333 0.0747000 0.008766667 6.144322e-01 1.291042e-08 FALSE TRUE
ENST00000582730 ENSG00000136450 HEK293_OSMI2_2hA HEK293_TMG_2hB SRSF1 protein_coding protein_coding 214.1002 110.0328 302.2643 19.00435 7.203718 1.457793 124.85523 93.257673 153.39237 18.9845953 6.681675 0.7178718 0.64818333 0.83993333 0.5070333 -0.332900000 1.291042e-08 1.291042e-08 FALSE TRUE
ENST00000583741 ENSG00000136450 HEK293_OSMI2_2hA HEK293_TMG_2hB SRSF1 protein_coding nonsense_mediated_decay 214.1002 110.0328 302.2643 19.00435 7.203718 1.457793 64.10412 3.512970 111.63932 1.8811975 2.656844 4.9860384 0.22787917 0.03676667 0.3694000 0.332633333 7.852621e-04 1.291042e-08 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.