ENSG00000136444

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000258955 ENSG00000136444 HEK293_OSMI2_2hA HEK293_TMG_2hB RSAD1 protein_coding protein_coding 37.40559 41.77172 25.73856 1.27374 0.8385625 -0.6983801 28.681909 35.228217 17.115353 0.6645170 0.5588655 -1.04100720 0.76072917 0.84403333 0.6649667 -0.17906667 3.996236e-10 3.996236e-10 FALSE TRUE
ENST00000509398 ENSG00000136444 HEK293_OSMI2_2hA HEK293_TMG_2hB RSAD1 protein_coding retained_intron 37.40559 41.77172 25.73856 1.27374 0.8385625 -0.6983801 2.105247 1.292575 2.076617 0.2685994 0.2345687 0.67979974 0.05747083 0.03066667 0.0806000 0.04993333 2.839766e-03 3.996236e-10   FALSE
ENST00000513650 ENSG00000136444 HEK293_OSMI2_2hA HEK293_TMG_2hB RSAD1 protein_coding retained_intron 37.40559 41.77172 25.73856 1.27374 0.8385625 -0.6983801 2.309497 1.912673 1.968916 0.3114363 0.1208480 0.04159735 0.06124167 0.04543333 0.0767000 0.03126667 7.273386e-02 3.996236e-10 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000136444 E001 9.9401829 0.0637791894 4.140879e-02 9.364208e-02 17 50478860 50478875 16 + 1.172 0.893 -1.026
ENSG00000136444 E002 18.2999908 0.0281891175 4.496265e-03 1.453268e-02 17 50478876 50478902 27 + 1.423 1.133 -1.019
ENSG00000136444 E003 60.3452908 0.0018508660 1.974598e-05 1.226256e-04 17 50478903 50479019 117 + 1.869 1.683 -0.626
ENSG00000136444 E004 10.3635942 0.0016473203 2.106542e-13 5.718271e-12 17 50479048 50479428 381 + 1.385 0.658 -2.710
ENSG00000136444 E005 124.3506113 0.0009577219 4.799657e-04 2.083451e-03 17 50479629 50479762 134 + 2.123 2.028 -0.321
ENSG00000136444 E006 202.4285398 0.0002357071 1.022194e-01 1.938939e-01 17 50479880 50480084 205 + 2.283 2.268 -0.049
ENSG00000136444 E007 18.9368290 0.0021707479 1.501150e-20 1.024454e-18 17 50480085 50480629 545 + 1.625 0.896 -2.581
ENSG00000136444 E008 80.6476059 0.0004473803 3.137809e-01 4.564220e-01 17 50482091 50482096 6 + 1.889 1.876 -0.045
ENSG00000136444 E009 244.6546698 0.0002379283 8.750910e-01 9.237489e-01 17 50482097 50482363 267 + 2.336 2.361 0.083
ENSG00000136444 E010 175.3441872 0.0055159021 5.151496e-01 6.498490e-01 17 50482364 50482456 93 + 2.209 2.208 -0.001
ENSG00000136444 E011 24.3445382 0.0115285319 6.516189e-05 3.567118e-04 17 50482457 50482642 186 + 1.565 1.254 -1.078
ENSG00000136444 E012 178.5954446 0.0025025677 9.386633e-01 9.654905e-01 17 50482643 50482706 64 + 2.199 2.224 0.084
ENSG00000136444 E013 242.6955794 0.0002051205 5.861549e-01 7.097723e-01 17 50483340 50483431 92 + 2.320 2.362 0.140
ENSG00000136444 E014 201.6167610 0.0002079891 8.484237e-01 9.058808e-01 17 50483432 50483487 56 + 2.246 2.280 0.113
ENSG00000136444 E015 20.0212819 0.0094525796 2.312297e-03 8.189218e-03 17 50483488 50483535 48 + 1.437 1.196 -0.841
ENSG00000136444 E016 201.2335981 0.0005348825 2.604205e-01 3.979500e-01 17 50483706 50483760 55 + 2.227 2.287 0.200
ENSG00000136444 E017 34.6425738 0.0206266678 1.027838e-02 2.945273e-02 17 50484029 50484441 413 + 1.648 1.445 -0.693
ENSG00000136444 E018 235.8824756 0.0001919216 1.734291e-01 2.926483e-01 17 50484442 50484545 104 + 2.293 2.356 0.208
ENSG00000136444 E019 20.0816926 0.0009531680 2.612916e-06 1.982892e-05 17 50484546 50484642 97 + 1.491 1.161 -1.150
ENSG00000136444 E020 17.7519289 0.0015146752 3.363356e-09 4.524124e-08 17 50484643 50484743 101 + 1.497 1.049 -1.578
ENSG00000136444 E021 227.0887016 0.0044854600 6.375625e-01 7.514265e-01 17 50484744 50484894 151 + 2.315 2.323 0.027
ENSG00000136444 E022 402.4057081 0.0022806929 7.543170e-01 8.399906e-01 17 50484895 50485178 284 + 2.557 2.576 0.062
ENSG00000136444 E023 926.9375864 0.0045074086 1.380967e-06 1.112425e-05 17 50485179 50485974 796 + 2.810 2.976 0.553
ENSG00000136444 E024 0.2214452 0.0395613033 1.000000e+00   17 50486912 50486947 36 + 0.001 0.093 7.491
ENSG00000136444 E025 0.5149111 0.0201746220 1.000000e+00 1.000000e+00 17 50487295 50487459 165 + 0.160 0.168 0.084