ENSG00000136270

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000258770 ENSG00000136270 HEK293_OSMI2_2hA HEK293_TMG_2hB TBRG4 protein_coding protein_coding 141.3029 219.0313 79.34434 9.938969 2.105818 -1.464822 73.923014 131.277016 30.973537 7.6639317 0.7367058 -2.08315049 0.49659167 0.59866667 0.39053333 -0.208133333 3.031394e-19 3.031394e-19 FALSE TRUE
ENST00000361278 ENSG00000136270 HEK293_OSMI2_2hA HEK293_TMG_2hB TBRG4 protein_coding protein_coding 141.3029 219.0313 79.34434 9.938969 2.105818 -1.464822 8.118551 10.531620 10.706058 0.3008911 0.7083047 0.02367772 0.06592083 0.04823333 0.13493333 0.086700000 1.622827e-16 3.031394e-19 FALSE TRUE
ENST00000478116 ENSG00000136270 HEK293_OSMI2_2hA HEK293_TMG_2hB TBRG4 protein_coding retained_intron 141.3029 219.0313 79.34434 9.938969 2.105818 -1.464822 9.071586 9.502286 6.606750 0.4217116 0.5422155 -0.52366922 0.07151250 0.04360000 0.08303333 0.039433333 2.192540e-05 3.031394e-19 FALSE FALSE
ENST00000484326 ENSG00000136270 HEK293_OSMI2_2hA HEK293_TMG_2hB TBRG4 protein_coding retained_intron 141.3029 219.0313 79.34434 9.938969 2.105818 -1.464822 6.732776 6.780826 6.161911 0.8980760 0.6963697 -0.13786972 0.05393333 0.03076667 0.07770000 0.046933333 6.636436e-05 3.031394e-19 FALSE TRUE
MSTRG.29827.2 ENSG00000136270 HEK293_OSMI2_2hA HEK293_TMG_2hB TBRG4 protein_coding   141.3029 219.0313 79.34434 9.938969 2.105818 -1.464822 8.077942 15.609073 3.242763 2.8613839 0.1834785 -2.26357105 0.05339167 0.07163333 0.04086667 -0.030766667 2.082372e-01 3.031394e-19 FALSE TRUE
MSTRG.29827.3 ENSG00000136270 HEK293_OSMI2_2hA HEK293_TMG_2hB TBRG4 protein_coding   141.3029 219.0313 79.34434 9.938969 2.105818 -1.464822 17.457138 24.671247 8.663116 1.1658346 0.2936559 -1.50879288 0.12140417 0.11280000 0.10953333 -0.003266667 9.269499e-01 3.031394e-19 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000136270 E001 8.370155 2.105520e-03 1.437640e-05 9.208078e-05 7 45100096 45100099 4 - 1.226 0.742 -1.809
ENSG00000136270 E002 19.741976 6.070015e-02 5.875967e-01 7.108593e-01 7 45100100 45100100 1 - 1.271 1.214 -0.200
ENSG00000136270 E003 1621.002887 1.149889e-03 2.150910e-06 1.661164e-05 7 45100101 45100426 326 - 3.025 3.122 0.323
ENSG00000136270 E004 1665.321360 9.404795e-04 2.253842e-04 1.072015e-03 7 45101258 45101372 115 - 3.057 3.129 0.238
ENSG00000136270 E005 1484.655657 7.765693e-04 8.991141e-05 4.745750e-04 7 45101503 45101599 97 - 3.006 3.079 0.245
ENSG00000136270 E006 771.413489 1.772030e-04 5.453874e-06 3.849697e-05 7 45101600 45101614 15 - 2.712 2.794 0.272
ENSG00000136270 E007 57.048912 4.370468e-04 9.555632e-11 1.691116e-09 7 45101774 45101824 51 - 1.901 1.576 -1.098
ENSG00000136270 E008 1748.579808 3.571872e-04 1.468686e-08 1.755779e-07 7 45101825 45102070 246 - 3.069 3.151 0.273
ENSG00000136270 E009 17.987791 7.990572e-03 3.116312e-02 7.421521e-02 7 45102281 45102346 66 - 1.337 1.131 -0.727
ENSG00000136270 E010 1153.726949 1.051889e-04 2.537599e-09 3.490203e-08 7 45102347 45102491 145 - 2.884 2.970 0.286
ENSG00000136270 E011 105.114802 3.969392e-04 3.477055e-17 1.580796e-15 7 45102492 45102690 199 - 2.159 1.841 -1.067
ENSG00000136270 E012 123.912043 2.644073e-04 4.919997e-17 2.193642e-15 7 45103031 45103332 302 - 2.209 1.916 -0.982
ENSG00000136270 E013 1137.712420 1.295180e-04 6.613651e-01 7.698155e-01 7 45103333 45103443 111 - 2.952 2.949 -0.010
ENSG00000136270 E014 153.927623 3.779446e-03 7.439340e-30 1.237367e-27 7 45103632 45104098 467 - 2.417 1.945 -1.577
ENSG00000136270 E015 1086.726002 9.709493e-05 1.402850e-03 5.315922e-03 7 45104099 45104208 110 - 2.890 2.937 0.159
ENSG00000136270 E016 764.281346 3.170174e-04 1.176061e-05 7.688366e-05 7 45104209 45104256 48 - 2.706 2.790 0.280
ENSG00000136270 E017 1229.406069 1.493555e-04 5.953537e-11 1.089634e-09 7 45104538 45104709 172 - 2.903 2.998 0.315
ENSG00000136270 E018 50.697326 7.651019e-03 9.821971e-04 3.899212e-03 7 45104710 45104746 37 - 1.774 1.553 -0.750
ENSG00000136270 E019 37.073260 5.606627e-04 1.333947e-22 1.137319e-20 7 45105286 45105440 155 - 1.866 1.296 -1.948
ENSG00000136270 E020 1917.482640 1.003410e-04 8.842959e-01 9.300629e-01 7 45105441 45105764 324 - 3.174 3.176 0.006
ENSG00000136270 E021 8.747664 1.873928e-03 1.863127e-12 4.367824e-11 7 45105765 45105967 203 - 1.372 0.616 -2.848
ENSG00000136270 E022 31.611722 5.054595e-03 5.132458e-32 9.911608e-30 7 45105968 45107464 1497 - 1.914 1.096 -2.818
ENSG00000136270 E023 4.444472 4.257842e-03 2.121328e-04 1.015847e-03 7 45107751 45107778 28 - 1.006 0.490 -2.130
ENSG00000136270 E024 687.781809 1.396728e-03 1.421669e-02 3.871812e-02 7 45108827 45108853 27 - 2.777 2.722 -0.181
ENSG00000136270 E025 729.310718 1.156800e-03 2.929444e-03 1.005643e-02 7 45108854 45108883 30 - 2.808 2.746 -0.208
ENSG00000136270 E026 720.917814 4.577270e-04 1.877444e-02 4.884118e-02 7 45108884 45108927 44 - 2.784 2.744 -0.135
ENSG00000136270 E027 497.232196 6.511161e-04 7.629236e-02 1.535664e-01 7 45108928 45108931 4 - 2.620 2.583 -0.122
ENSG00000136270 E028 1244.899880 1.465281e-04 2.621985e-12 6.012918e-11 7 45108932 45109083 152 - 3.056 2.970 -0.288
ENSG00000136270 E029 1119.676349 4.334744e-04 5.006531e-10 7.834729e-09 7 45109084 45109222 139 - 3.016 2.923 -0.309
ENSG00000136270 E030 538.593146 1.039804e-03 9.052481e-04 3.632478e-03 7 45109223 45109243 21 - 2.684 2.610 -0.249
ENSG00000136270 E031 575.809236 1.068867e-03 1.962111e-03 7.107654e-03 7 45109244 45109287 44 - 2.708 2.640 -0.227
ENSG00000136270 E032 2.737637 1.370748e-02 4.121112e-01 5.556687e-01 7 45110745 45110877 133 - 0.607 0.464 -0.671
ENSG00000136270 E033 11.301605 1.560733e-03 9.051924e-01 9.436669e-01 7 45111376 45111642 267 - 1.006 0.994 -0.043
ENSG00000136270 E034 391.773726 1.732379e-03 4.250398e-01 5.679796e-01 7 45111643 45111663 21 - 2.501 2.483 -0.062
ENSG00000136270 E035 391.208787 6.029121e-04 3.562808e-02 8.286078e-02 7 45111664 45111876 213 - 2.523 2.475 -0.160
ENSG00000136270 E036 2.025113 7.198424e-03 8.136343e-01 8.818509e-01 7 45111877 45112047 171 - 0.451 0.409 -0.224