ENSG00000136231

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000258729 ENSG00000136231 HEK293_OSMI2_2hA HEK293_TMG_2hB IGF2BP3 protein_coding protein_coding 12.26146 4.056585 20.13353 0.36358 1.313778 2.308426 7.1002888 2.750384 13.6410012 0.2629300 0.5133787 2.306066 0.56044167 0.7012000 0.68103333 -0.02016667 0.90690402 0.02627553 FALSE  
ENST00000498363 ENSG00000136231 HEK293_OSMI2_2hA HEK293_TMG_2hB IGF2BP3 protein_coding retained_intron 12.26146 4.056585 20.13353 0.36358 1.313778 2.308426 0.8680484 0.000000 1.2063136 0.0000000 0.6584355 6.926371 0.06248333 0.0000000 0.06206667 0.06206667 0.03030743 0.02627553 FALSE  
ENST00000619562 ENSG00000136231 HEK293_OSMI2_2hA HEK293_TMG_2hB IGF2BP3 protein_coding protein_coding 12.26146 4.056585 20.13353 0.36358 1.313778 2.308426 1.3652928 0.000000 1.4209011 0.0000000 0.7312629 7.160780 0.07293750 0.0000000 0.06690000 0.06690000 0.38237641 0.02627553 FALSE  
MSTRG.29573.1 ENSG00000136231 HEK293_OSMI2_2hA HEK293_TMG_2hB IGF2BP3 protein_coding   12.26146 4.056585 20.13353 0.36358 1.313778 2.308426 0.6330459 1.125289 0.4225053 0.6287713 0.4225053 -1.392270 0.15840833 0.2533333 0.02150000 -0.23183333 0.31196740 0.02627553 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000136231 E001 238.6179801 0.0041260847 7.396700e-50 4.448516e-47 7 23310209 23311126 918 - 2.093 2.672 1.930
ENSG00000136231 E002 77.9406177 0.0027049199 1.101799e-10 1.930205e-09 7 23311127 23311240 114 - 1.695 2.063 1.238
ENSG00000136231 E003 336.4249117 0.0037388284 9.082368e-06 6.094168e-05 7 23311241 23312372 1132 - 2.378 2.569 0.635
ENSG00000136231 E004 86.0280617 0.0017990140 9.857594e-01 9.951198e-01 7 23312373 23312460 88 - 1.825 1.873 0.162
ENSG00000136231 E005 82.5646384 0.0003652966 9.235731e-01 9.558490e-01 7 23312735 23312848 114 - 1.806 1.851 0.151
ENSG00000136231 E006 86.3857592 0.0003456103 2.709038e-01 4.095628e-01 7 23313522 23313653 132 - 1.836 1.832 -0.013
ENSG00000136231 E007 49.7978669 0.0004472299 3.914939e-02 8.945885e-02 7 23317639 23317692 54 - 1.616 1.528 -0.298
ENSG00000136231 E008 36.2965463 0.0006017135 1.140413e-01 2.114095e-01 7 23317693 23317713 21 - 1.480 1.408 -0.248
ENSG00000136231 E009 3.6801073 0.0581471785 5.508233e-01 6.803794e-01 7 23317714 23317833 120 - 0.601 0.510 -0.421
ENSG00000136231 E010 71.6596440 0.0035830572 5.240865e-02 1.134469e-01 7 23319138 23319254 117 - 1.769 1.699 -0.236
ENSG00000136231 E011 36.5665295 0.0013060705 3.381896e-04 1.533338e-03 7 23342064 23342065 2 - 1.511 1.258 -0.878
ENSG00000136231 E012 49.0084240 0.0043536489 9.597109e-03 2.777191e-02 7 23342066 23342095 30 - 1.621 1.476 -0.497
ENSG00000136231 E013 65.3979582 0.0013226889 3.897209e-03 1.285613e-02 7 23342096 23342189 94 - 1.741 1.614 -0.429
ENSG00000136231 E014 87.4361940 0.0026987968 8.890280e-04 3.575874e-03 7 23343718 23343846 129 - 1.867 1.730 -0.466
ENSG00000136231 E015 47.9248103 0.0057411482 1.629892e-01 2.790382e-01 7 23343847 23343853 7 - 1.599 1.545 -0.184
ENSG00000136231 E016 89.3439540 0.0890023090 7.139633e-02 1.456535e-01 7 23345940 23346062 123 - 1.883 1.682 -0.680
ENSG00000136231 E017 0.2903454 0.2793744779 1.000000e+00   7 23346063 23346161 99 - 0.108 0.000 -10.188
ENSG00000136231 E018 47.1916677 0.0004788729 9.599448e-05 5.032617e-04 7 23347600 23347630 31 - 1.617 1.381 -0.809
ENSG00000136231 E019 40.2863873 0.0005798390 1.901269e-03 6.918876e-03 7 23347631 23347648 18 - 1.544 1.354 -0.656
ENSG00000136231 E020 39.0349504 0.0051353083 1.566162e-02 4.198335e-02 7 23347649 23347662 14 - 1.526 1.367 -0.546
ENSG00000136231 E021 44.8444380 0.0050556352 6.078395e-04 2.561753e-03 7 23347663 23347698 36 - 1.594 1.354 -0.826
ENSG00000136231 E022 38.9805139 0.0054068347 2.739584e-04 1.273801e-03 7 23347699 23347723 25 - 1.540 1.258 -0.979
ENSG00000136231 E023 32.7421630 0.0140774746 2.630330e-03 9.160891e-03 7 23347724 23347734 11 - 1.466 1.180 -0.998
ENSG00000136231 E024 0.1451727 0.0426727046 1.000000e+00   7 23347735 23347812 78 - 0.058 0.000 -9.243
ENSG00000136231 E025 54.1833823 0.0004418253 2.490219e-05 1.511049e-04 7 23351305 23351386 82 - 1.673 1.432 -0.823
ENSG00000136231 E026 43.4275588 0.0006300103 3.436663e-05 2.013812e-04 7 23351387 23351443 57 - 1.583 1.309 -0.946
ENSG00000136231 E027 36.9420901 0.0038715977 2.677534e-04 1.248372e-03 7 23351444 23351479 36 - 1.517 1.240 -0.961
ENSG00000136231 E028 33.3823760 0.0132069948 1.759853e-03 6.471251e-03 7 23351480 23351505 26 - 1.473 1.182 -1.016
ENSG00000136231 E029 49.7038125 0.0038408217 5.772282e-03 1.801432e-02 7 23351506 23351586 81 - 1.626 1.467 -0.544
ENSG00000136231 E030 0.2924217 0.0290785164 1.000000e+00   7 23361341 23361533 193 - 0.109 0.000 -10.241
ENSG00000136231 E031 39.8874714 0.0013039989 8.598761e-03 2.528536e-02 7 23361534 23361597 64 - 1.535 1.381 -0.530
ENSG00000136231 E032 34.5155295 0.0055198746 2.179179e-02 5.524110e-02 7 23361690 23361741 52 - 1.475 1.323 -0.527
ENSG00000136231 E033 0.1472490 0.0434822266 1.000000e+00   7 23362527 23362675 149 - 0.058 0.000 -9.243
ENSG00000136231 E034 0.0000000       7 23394584 23394677 94 -      
ENSG00000136231 E035 0.2987644 0.0296600054 1.000000e+00   7 23402245 23402570 326 - 0.109 0.000 -10.242
ENSG00000136231 E036 1.0246279 0.0167164695 2.165909e-01 3.462950e-01 7 23402571 23402585 15 - 0.300 0.000 -12.041
ENSG00000136231 E037 0.1515154 0.0430972597 1.000000e+00   7 23402586 23402710 125 - 0.058 0.000 -9.244
ENSG00000136231 E038 0.8751888 0.1129508792 3.262266e-01 4.696678e-01 7 23405019 23405110 92 - 0.268 0.000 -11.807
ENSG00000136231 E039 5.7520489 0.0030035289 8.200511e-01 8.864531e-01 7 23413244 23415565 2322 - 0.733 0.738 0.019
ENSG00000136231 E040 1.0373134 0.0118036831 2.132523e-01 3.423758e-01 7 23416129 23416219 91 - 0.300 0.000 -12.042
ENSG00000136231 E041 32.4771959 0.0202734790 7.471055e-03 2.243982e-02 7 23418776 23418824 49 - 1.460 1.201 -0.904
ENSG00000136231 E042 0.2934659 0.0282851402 2.618406e-01   7 23431169 23431303 135 - 0.058 0.241 2.385
ENSG00000136231 E043 0.6934950 0.0597089300 1.439503e-01 2.533911e-01 7 23467772 23467926 155 - 0.109 0.397 2.395
ENSG00000136231 E044 44.7833810 0.0110008899 3.126636e-02 7.442102e-02 7 23468482 23468542 61 - 1.578 1.434 -0.494
ENSG00000136231 E045 2.8452135 0.0305249200 1.221013e-01 2.229263e-01 7 23469236 23469502 267 - 0.552 0.242 -1.782
ENSG00000136231 E046 59.3146266 0.0143312263 2.818089e-02 6.836971e-02 7 23469936 23470081 146 - 1.697 1.551 -0.498
ENSG00000136231 E047 52.5753669 0.0004399217 1.207186e-02 3.370686e-02 7 23470082 23470216 135 - 1.643 1.528 -0.392
ENSG00000136231 E048 42.3000808 0.0005698571 2.682057e-01 4.066455e-01 7 23470217 23470491 275 - 1.537 1.509 -0.097
ENSG00000136231 E049 0.3030308 0.3961675822 1.000000e+00   7 23471032 23471137 106 - 0.109 0.000 -10.154