ENSG00000136146

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000258648 ENSG00000136146 HEK293_OSMI2_2hA HEK293_TMG_2hB MED4 protein_coding protein_coding 43.41867 28.92013 66.06276 2.31015 1.165938 1.191483 12.81148 9.274377 17.50716 0.9435227 0.9091624 0.9158919 0.2997708 0.3197667 0.2647333 -0.05503333 0.1078327412 5.998948e-05 FALSE TRUE
ENST00000378586 ENSG00000136146 HEK293_OSMI2_2hA HEK293_TMG_2hB MED4 protein_coding protein_coding 43.41867 28.92013 66.06276 2.31015 1.165938 1.191483 16.67496 9.181637 27.98706 0.9601877 0.9966862 1.6068815 0.3684458 0.3163333 0.4233667 0.10703333 0.0002319998 5.998948e-05 FALSE TRUE
MSTRG.8736.2 ENSG00000136146 HEK293_OSMI2_2hA HEK293_TMG_2hB MED4 protein_coding   43.41867 28.92013 66.06276 2.31015 1.165938 1.191483 12.13763 8.687720 18.78237 0.2832142 0.4588453 1.1114374 0.2806042 0.3033667 0.2847333 -0.01863333 0.8325652018 5.998948e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000136146 E001 2.503585 0.0086182497 1.400981e-01 0.2481587118 13 48052420 48053322 903 - 0.619 0.364 -1.268
ENSG00000136146 E002 1.682445 0.5274532542 3.570910e-01 0.5015107404 13 48053323 48053355 33 - 0.343 0.541 1.042
ENSG00000136146 E003 34.040039 0.0046676721 1.395190e-04 0.0007014160 13 48053356 48053491 136 - 1.415 1.677 0.897
ENSG00000136146 E004 401.811623 0.0021141598 4.736203e-02 0.1044746260 13 48075724 48076967 1244 - 2.576 2.632 0.187
ENSG00000136146 E005 320.417365 0.0002318186 8.700481e-02 0.1705993804 13 48076968 48077152 185 - 2.486 2.524 0.128
ENSG00000136146 E006 147.572809 0.0005217731 5.871922e-01 0.7105332334 13 48077153 48077170 18 - 2.155 2.175 0.067
ENSG00000136146 E007 372.008239 0.0002052175 5.506969e-01 0.6802986434 13 48077171 48077311 141 - 2.567 2.560 -0.025
ENSG00000136146 E008 330.044130 0.0001568679 5.035776e-01 0.6395643690 13 48079844 48079975 132 - 2.506 2.523 0.057
ENSG00000136146 E009 260.339033 0.0002413917 2.085993e-02 0.0532924837 13 48081645 48081731 87 - 2.390 2.446 0.186
ENSG00000136146 E010 242.179013 0.0011693191 5.099945e-01 0.6452571562 13 48083371 48083428 58 - 2.373 2.394 0.073
ENSG00000136146 E011 454.869943 0.0001587230 9.109906e-02 0.1768591253 13 48086282 48086452 171 - 2.662 2.638 -0.081
ENSG00000136146 E012 300.988885 0.0002254929 2.280629e-04 0.0010835045 13 48090352 48090418 67 - 2.501 2.427 -0.248
ENSG00000136146 E013 2.955943 0.0049496859 8.214730e-03 0.0243290436 13 48093548 48093687 140 - 0.730 0.273 -2.322
ENSG00000136146 E014 185.680257 0.0014972016 1.350403e-01 0.2411463583 13 48094954 48095059 106 - 2.281 2.234 -0.156
ENSG00000136146 E015 98.487620 0.0006786110 1.229071e-06 0.0000100135 13 48095060 48095131 72 - 2.054 1.866 -0.630