ENSG00000136144

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000258646 ENSG00000136144 HEK293_OSMI2_2hA HEK293_TMG_2hB RCBTB1 protein_coding protein_coding 10.08339 4.060218 15.97968 0.578443 0.1286005 1.973963 2.98672667 2.443884030 3.481403 0.372765793 1.5538331 0.5087398 0.380070833 0.608300000 0.21640000 -0.39190000 0.058278537 0.005088228 FALSE TRUE
ENST00000378302 ENSG00000136144 HEK293_OSMI2_2hA HEK293_TMG_2hB RCBTB1 protein_coding protein_coding 10.08339 4.060218 15.97968 0.578443 0.1286005 1.973963 5.53719529 1.149836115 9.422435 0.229193594 1.1758219 3.0237094 0.481762500 0.278533333 0.59046667 0.31193333 0.006929256 0.005088228 FALSE TRUE
MSTRG.8767.1 ENSG00000136144 HEK293_OSMI2_2hA HEK293_TMG_2hB RCBTB1 protein_coding   10.08339 4.060218 15.97968 0.578443 0.1286005 1.973963 0.03864472 0.236514684 0.000000 0.236514684 0.0000000 -4.6236017 0.009454167 0.060466667 0.00000000 -0.06046667 0.585741961 0.005088228 FALSE TRUE
MSTRG.8767.4 ENSG00000136144 HEK293_OSMI2_2hA HEK293_TMG_2hB RCBTB1 protein_coding   10.08339 4.060218 15.97968 0.578443 0.1286005 1.973963 0.30043944 0.008993983 1.120923 0.008993983 0.4641655 5.8958150 0.020275000 0.001766667 0.07063333 0.06886667 0.005088228 0.005088228 FALSE TRUE
MSTRG.8767.8 ENSG00000136144 HEK293_OSMI2_2hA HEK293_TMG_2hB RCBTB1 protein_coding   10.08339 4.060218 15.97968 0.578443 0.1286005 1.973963 0.67889686 0.124428132 1.164764 0.071394405 0.3158478 3.1274637 0.057504167 0.027433333 0.07260000 0.04516667 0.362205134 0.005088228 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000136144 E001 347.5494852 0.0002432768 1.762830e-46 8.811687e-44 13 49531946 49533550 1605 - 2.402 2.667 0.882
ENSG00000136144 E002 79.4575139 0.0003230264 2.442677e-01 3.792196e-01 13 49533551 49533726 176 - 1.833 1.888 0.186
ENSG00000136144 E003 105.1789177 0.0003414146 6.453425e-02 1.342715e-01 13 49533727 49533917 191 - 1.985 1.915 -0.237
ENSG00000136144 E004 114.0747003 0.0002994873 2.960236e-01 4.371924e-01 13 49533918 49534216 299 - 2.013 1.978 -0.119
ENSG00000136144 E005 30.4331146 0.0183837003 3.279000e-01 4.713713e-01 13 49534217 49534262 46 - 1.420 1.510 0.310
ENSG00000136144 E006 1.6491090 0.2222319342 5.649894e-01 6.921730e-01 13 49537870 49538083 214 - 0.344 0.460 0.642
ENSG00000136144 E007 0.0000000       13 49539375 49539503 129 -      
ENSG00000136144 E008 60.7300098 0.0043738375 7.157659e-01 8.116146e-01 13 49540876 49541006 131 - 1.741 1.722 -0.065
ENSG00000136144 E009 69.3194063 0.0004345400 2.324425e-01 3.653492e-01 13 49541676 49541827 152 - 1.803 1.748 -0.188
ENSG00000136144 E010 0.0000000       13 49542044 49542186 143 -      
ENSG00000136144 E011 57.4132320 0.0019564225 8.634191e-02 1.695470e-01 13 49544737 49544863 127 - 1.733 1.635 -0.334
ENSG00000136144 E012 7.3390322 0.0026983694 6.636852e-01 7.716886e-01 13 49547021 49547171 151 - 0.860 0.920 0.230
ENSG00000136144 E013 66.4563597 0.0004035111 4.172450e-01 5.606070e-01 13 49549458 49549648 191 - 1.780 1.743 -0.126
ENSG00000136144 E014 3.1106779 0.0050062603 8.059806e-01 8.766699e-01 13 49550411 49550464 54 - 0.577 0.537 -0.183
ENSG00000136144 E015 0.7405115 0.3350762323 3.926004e-01 5.365530e-01 13 49551171 49551325 155 - 0.271 0.001 -9.325
ENSG00000136144 E016 66.6995354 0.0005161216 7.332105e-03 2.208199e-02 13 49551326 49551468 143 - 1.802 1.665 -0.466
ENSG00000136144 E017 70.6857268 0.0003690825 3.544340e-04 1.597281e-03 13 49552178 49552285 108 - 1.839 1.659 -0.609
ENSG00000136144 E018 81.3039600 0.0004153751 3.796963e-05 2.200707e-04 13 49555515 49555673 159 - 1.903 1.708 -0.660
ENSG00000136144 E019 83.3456521 0.0003403978 3.195460e-05 1.888713e-04 13 49559918 49560084 167 - 1.913 1.718 -0.656
ENSG00000136144 E020 73.2576175 0.0003371906 5.354523e-07 4.708714e-06 13 49566618 49566768 151 - 1.868 1.609 -0.876
ENSG00000136144 E021 65.9132800 0.0015235698 6.566786e-04 2.740155e-03 13 49567154 49567320 167 - 1.809 1.622 -0.635
ENSG00000136144 E022 3.3235903 0.0063977290 8.683012e-01 9.193118e-01 13 49567321 49567323 3 - 0.577 0.608 0.140
ENSG00000136144 E023 37.5005983 0.0088163843 1.347415e-01 2.407308e-01 13 49580505 49580580 76 - 1.555 1.435 -0.411
ENSG00000136144 E024 17.9891332 0.0010618228 6.806040e-01 7.844637e-01 13 49580581 49580584 4 - 1.229 1.195 -0.122
ENSG00000136144 E025 29.0679347 0.0007865526 3.454565e-01 4.895537e-01 13 49585444 49585654 211 - 1.440 1.373 -0.232