ENSG00000136003

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000311893 ENSG00000136003 HEK293_OSMI2_2hA HEK293_TMG_2hB ISCU protein_coding protein_coding 59.55038 73.5406 55.58652 3.676174 0.4894754 -0.4037426 48.116864 64.625245 42.658647 2.9616940 0.79759939 -0.5991447 0.79995833 0.87940000 0.76756667 -0.11183333 1.169208e-03 6.504199e-07 FALSE TRUE
ENST00000392807 ENSG00000136003 HEK293_OSMI2_2hA HEK293_TMG_2hB ISCU protein_coding protein_coding 59.55038 73.5406 55.58652 3.676174 0.4894754 -0.4037426 4.281449 4.053363 5.089431 0.6280266 0.92618204 0.3276620 0.07234583 0.05463333 0.09130000 0.03666667 2.440955e-01 6.504199e-07 FALSE TRUE
ENST00000545932 ENSG00000136003 HEK293_OSMI2_2hA HEK293_TMG_2hB ISCU protein_coding retained_intron 59.55038 73.5406 55.58652 3.676174 0.4894754 -0.4037426 2.891208 1.694056 3.452781 0.2491179 0.02567908 1.0229585 0.05257917 0.02300000 0.06213333 0.03913333 6.504199e-07 6.504199e-07 TRUE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000136003 E001 12.315533 0.0088713258 4.076741e-02 9.244878e-02 12 108562582 108562595 14 + 1.218 1.012 -0.743
ENSG00000136003 E002 14.882629 0.0058575674 3.239446e-02 7.661811e-02 12 108562596 108562600 5 + 1.291 1.097 -0.689
ENSG00000136003 E003 25.255040 0.0195475895 3.682850e-01 5.126979e-01 12 108562601 108562605 5 + 1.451 1.369 -0.285
ENSG00000136003 E004 42.470807 0.0067014400 1.431284e-01 2.522794e-01 12 108562606 108562609 4 + 1.677 1.584 -0.317
ENSG00000136003 E005 64.383425 0.0123702965 5.826634e-02 1.236556e-01 12 108562610 108562616 7 + 1.874 1.747 -0.428
ENSG00000136003 E006 239.719356 0.0022288340 2.366288e-01 3.702773e-01 12 108562617 108562736 120 + 2.387 2.355 -0.107
ENSG00000136003 E007 134.623660 0.0127875754 7.042681e-15 2.374893e-13 12 108562737 108563987 1251 + 2.347 1.874 -1.587
ENSG00000136003 E008 24.008349 0.0050687218 9.176365e-09 1.139210e-07 12 108563988 108564059 72 + 1.602 1.167 -1.510
ENSG00000136003 E009 58.352118 0.0016280190 5.951065e-11 1.089337e-09 12 108564060 108564155 96 + 1.926 1.613 -1.058
ENSG00000136003 E010 37.105961 0.0005892759 6.229785e-13 1.580997e-11 12 108564156 108564278 123 + 1.770 1.364 -1.390
ENSG00000136003 E011 438.053924 0.0002130218 4.281637e-01 5.709583e-01 12 108564279 108564392 114 + 2.633 2.626 -0.023
ENSG00000136003 E012 5.835530 0.0027801912 1.560986e-02 4.186706e-02 12 108564999 108565320 322 + 0.987 0.691 -1.154
ENSG00000136003 E013 527.787290 0.0001186676 1.388805e-02 3.797212e-02 12 108565321 108565431 111 + 2.683 2.727 0.147
ENSG00000136003 E014 478.147059 0.0005925312 1.712177e-08 2.021355e-07 12 108567190 108567268 79 + 2.597 2.709 0.374
ENSG00000136003 E015 18.322362 0.0011104017 3.046207e-03 1.040242e-02 12 108567269 108567657 389 + 1.402 1.176 -0.793
ENSG00000136003 E016 10.012445 0.0017280322 1.281590e-02 3.548003e-02 12 108567658 108567733 76 + 1.165 0.918 -0.906
ENSG00000136003 E017 5.915942 0.0047336498 3.829672e-02 8.790664e-02 12 108567734 108567743 10 + 0.970 0.716 -0.990
ENSG00000136003 E018 7.676708 0.0508129076 3.319471e-01 4.755761e-01 12 108567744 108567757 14 + 1.003 0.871 -0.498
ENSG00000136003 E019 10.261876 0.0152366601 5.997412e-02 1.265613e-01 12 108567758 108567857 100 + 1.154 0.946 -0.757
ENSG00000136003 E020 7.167349 0.0023825145 1.024667e-01 1.942689e-01 12 108567858 108567919 62 + 1.004 0.819 -0.700
ENSG00000136003 E021 10.281644 0.0017206876 9.788577e-01 9.908412e-01 12 108567920 108568016 97 + 1.035 1.044 0.033
ENSG00000136003 E022 45.085679 0.0005616709 6.530535e-01 7.636364e-01 12 108568017 108568830 814 + 1.663 1.647 -0.056
ENSG00000136003 E023 965.403468 0.0016974529 1.705033e-09 2.420483e-08 12 108568831 108569368 538 + 2.899 3.013 0.379