ENSG00000135966

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000393359 ENSG00000135966 HEK293_OSMI2_2hA HEK293_TMG_2hB TGFBRAP1 protein_coding protein_coding 12.24302 13.48203 11.1493 0.5647854 0.143086 -0.2738601 1.848584 0.000000 6.2862520 0.0000000 0.8696677 9.298349 0.1640375 0.00000000 0.5622000 0.56220000 5.545854e-23 5.545854e-23 FALSE TRUE
ENST00000595531 ENSG00000135966 HEK293_OSMI2_2hA HEK293_TMG_2hB TGFBRAP1 protein_coding protein_coding 12.24302 13.48203 11.1493 0.5647854 0.143086 -0.2738601 7.382731 11.796089 1.6129089 0.2209398 0.8825674 -2.862877 0.5848333 0.87670000 0.1464000 -0.73030000 2.948557e-02 5.545854e-23 FALSE TRUE
MSTRG.18973.3 ENSG00000135966 HEK293_OSMI2_2hA HEK293_TMG_2hB TGFBRAP1 protein_coding   12.24302 13.48203 11.1493 0.5647854 0.143086 -0.2738601 1.434283 1.318974 0.2296141 0.4271631 0.2296141 -2.471528 0.1173417 0.09553333 0.0201000 -0.07543333 1.456673e-01 5.545854e-23 TRUE TRUE
MSTRG.18973.4 ENSG00000135966 HEK293_OSMI2_2hA HEK293_TMG_2hB TGFBRAP1 protein_coding   12.24302 13.48203 11.1493 0.5647854 0.143086 -0.2738601 1.315338 0.265251 3.0205276 0.1746003 0.1979480 3.460749 0.1124875 0.02076667 0.2713667 0.25060000 3.267486e-03 5.545854e-23 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000135966 E001 3.0485914 0.0056324855 4.147592e-02 9.376477e-02 2 105264391 105264413 23 - 0.329 0.693 1.797
ENSG00000135966 E002 575.5192379 0.0051763493 4.317714e-07 3.868278e-06 2 105264414 105266086 1673 - 2.618 2.813 0.649
ENSG00000135966 E003 184.7952389 0.0044138056 2.640460e-04 1.233119e-03 2 105266087 105266225 139 - 2.136 2.317 0.605
ENSG00000135966 E004 366.8970664 0.0002334269 1.079800e-06 8.899153e-06 2 105266226 105266883 658 - 2.474 2.598 0.413
ENSG00000135966 E005 144.2640701 0.0002863093 7.448494e-01 8.330344e-01 2 105266884 105267120 237 - 2.136 2.157 0.070
ENSG00000135966 E006 65.6335342 0.0003923208 6.438920e-01 7.563315e-01 2 105267121 105267158 38 - 1.806 1.816 0.034
ENSG00000135966 E007 29.3132723 0.0006662416 2.826720e-01 4.227061e-01 2 105267159 105267196 38 - 1.494 1.455 -0.134
ENSG00000135966 E008 182.4661452 0.0014287117 3.709756e-02 8.565745e-02 2 105267197 105267559 363 - 2.272 2.239 -0.111
ENSG00000135966 E009 174.1299851 0.0037161849 9.437314e-02 1.819437e-01 2 105269272 105269705 434 - 2.250 2.216 -0.115
ENSG00000135966 E010 92.9040589 0.0003748229 4.038482e-01 5.476228e-01 2 105272855 105273014 160 - 1.918 1.981 0.208
ENSG00000135966 E011 104.1872403 0.0003403842 2.024927e-01 3.293325e-01 2 105273544 105273690 147 - 2.017 2.003 -0.048
ENSG00000135966 E012 109.4916835 0.0009238612 6.427717e-03 1.973436e-02 2 105275560 105275703 144 - 2.072 2.004 -0.227
ENSG00000135966 E013 59.6847967 0.0004410286 7.709839e-02 1.548402e-01 2 105277614 105277671 58 - 1.801 1.750 -0.171
ENSG00000135966 E014 168.7087257 0.0010062517 2.421706e-02 6.025939e-02 2 105280382 105280723 342 - 2.240 2.202 -0.126
ENSG00000135966 E015 73.0992030 0.0063021959 6.188166e-01 7.363648e-01 2 105284316 105284398 83 - 1.855 1.857 0.008
ENSG00000135966 E016 105.9778334 0.0056868337 2.583624e-01 3.956063e-01 2 105296356 105296510 155 - 2.032 2.005 -0.090
ENSG00000135966 E017 107.0090906 0.0005812565 1.823360e-04 8.879202e-04 2 105298511 105298705 195 - 2.080 1.979 -0.338
ENSG00000135966 E018 119.9094188 0.0003166940 4.918895e-10 7.709688e-09 2 105307614 105307917 304 - 2.167 1.998 -0.568
ENSG00000135966 E019 92.6946392 0.0003042213 3.285518e-16 1.324886e-14 2 105307918 105308315 398 - 2.104 1.838 -0.892
ENSG00000135966 E020 6.6321554 0.0321196396 1.468345e-03 5.534470e-03 2 105308316 105308318 3 - 1.090 0.665 -1.641
ENSG00000135966 E021 0.0000000       2 105308319 105308321 3 -      
ENSG00000135966 E022 0.5138669 0.0203237348 3.773024e-01 5.216289e-01 2 105328280 105328313 34 - 0.244 0.108 -1.425
ENSG00000135966 E023 15.8330790 0.0016172053 1.269952e-16 5.395572e-15 2 105329625 105329735 111 - 1.515 0.802 -2.572