ENSG00000135945

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000258428 ENSG00000135945 HEK293_OSMI2_2hA HEK293_TMG_2hB REV1 protein_coding protein_coding 8.960835 3.041732 13.13628 0.3806133 0.2994986 2.106955 1.3041248 0.00000000 1.9716579 0.00000000 0.38796445 7.6305641 0.20515000 0.00000000 0.15146667 0.15146667 4.438682e-07 7.05079e-12 FALSE TRUE
ENST00000393445 ENSG00000135945 HEK293_OSMI2_2hA HEK293_TMG_2hB REV1 protein_coding protein_coding 8.960835 3.041732 13.13628 0.3806133 0.2994986 2.106955 1.4262372 1.53713453 2.3529042 0.03779768 0.43638044 0.6109625 0.16525833 0.52443333 0.17786667 -0.34656667 1.784458e-03 7.05079e-12 FALSE TRUE
ENST00000413697 ENSG00000135945 HEK293_OSMI2_2hA HEK293_TMG_2hB REV1 protein_coding nonsense_mediated_decay 8.960835 3.041732 13.13628 0.3806133 0.2994986 2.106955 0.6530328 0.25098383 1.0777927 0.04445028 0.12199839 2.0593713 0.07383750 0.08320000 0.08170000 -0.00150000 1.000000e+00 7.05079e-12 TRUE TRUE
ENST00000465086 ENSG00000135945 HEK293_OSMI2_2hA HEK293_TMG_2hB REV1 protein_coding retained_intron 8.960835 3.041732 13.13628 0.3806133 0.2994986 2.106955 0.5381273 0.09579541 0.5549112 0.04923047 0.11606847 2.4167471 0.05730000 0.02793333 0.04186667 0.01393333 8.413992e-01 7.05079e-12   FALSE
ENST00000465835 ENSG00000135945 HEK293_OSMI2_2hA HEK293_TMG_2hB REV1 protein_coding processed_transcript 8.960835 3.041732 13.13628 0.3806133 0.2994986 2.106955 1.2683967 0.00000000 2.3621992 0.00000000 0.27169629 7.8900813 0.09545000 0.00000000 0.17916667 0.17916667 7.050790e-12 7.05079e-12 FALSE TRUE
ENST00000473819 ENSG00000135945 HEK293_OSMI2_2hA HEK293_TMG_2hB REV1 protein_coding retained_intron 8.960835 3.041732 13.13628 0.3806133 0.2994986 2.106955 0.5450340 0.31413781 0.8213495 0.21004270 0.27813474 1.3588478 0.05613333 0.09080000 0.06336667 -0.02743333 9.826716e-01 7.05079e-12 FALSE TRUE
ENST00000477121 ENSG00000135945 HEK293_OSMI2_2hA HEK293_TMG_2hB REV1 protein_coding retained_intron 8.960835 3.041732 13.13628 0.3806133 0.2994986 2.106955 0.4003263 0.10192140 0.2419204 0.05154200 0.05111468 1.1704822 0.05014583 0.02976667 0.01856667 -0.01120000 5.926730e-01 7.05079e-12 FALSE FALSE
MSTRG.18938.4 ENSG00000135945 HEK293_OSMI2_2hA HEK293_TMG_2hB REV1 protein_coding   8.960835 3.041732 13.13628 0.3806133 0.2994986 2.106955 0.7816736 0.25605283 1.4099864 0.01138655 0.28133562 2.4160924 0.08187917 0.08830000 0.10813333 0.01983333 8.715831e-01 7.05079e-12 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000135945 E001 0.2934659 0.0291156507 3.516962e-01   2 99400475 99400476 2 - 0.069 0.208 1.839
ENSG00000135945 E002 19.1914916 0.0009837793 5.510794e-03 1.731579e-02 2 99400477 99400775 299 - 1.179 1.409 0.805
ENSG00000135945 E003 17.3385588 0.0135613208 1.699729e-01 2.881688e-01 2 99400776 99400887 112 - 1.164 1.303 0.490
ENSG00000135945 E004 11.3569738 0.0021376646 1.928894e-01 3.174004e-01 2 99400888 99400937 50 - 1.005 1.144 0.504
ENSG00000135945 E005 6.2941521 0.0369306362 7.105192e-03 2.150011e-02 2 99400938 99400949 12 - 0.680 1.078 1.533
ENSG00000135945 E006 47.8988947 0.0005279878 1.110425e-11 2.296330e-10 2 99400950 99401285 336 - 1.511 1.867 1.207
ENSG00000135945 E007 40.1882301 0.0012948811 6.627057e-08 6.969834e-07 2 99401286 99401352 67 - 1.452 1.770 1.082
ENSG00000135945 E008 3.6128400 0.0080603017 2.372399e-02 5.924249e-02 2 99401470 99402243 774 - 0.510 0.854 1.460
ENSG00000135945 E009 21.9924939 0.0109808726 1.018636e-01 1.933351e-01 2 99402244 99402258 15 - 1.260 1.409 0.522
ENSG00000135945 E010 32.8608703 0.0209376206 9.250997e-02 1.790405e-01 2 99402259 99402346 88 - 1.425 1.594 0.581
ENSG00000135945 E011 6.5105804 0.0025358806 5.048401e-01 6.407110e-01 2 99402347 99402529 183 - 0.801 0.891 0.346
ENSG00000135945 E012 5.5766992 0.0029261374 7.329156e-01 8.242674e-01 2 99402530 99402643 114 - 0.777 0.725 -0.210
ENSG00000135945 E013 57.7445479 0.0005131928 3.396084e-01 4.835393e-01 2 99402644 99402800 157 - 1.697 1.747 0.166
ENSG00000135945 E014 43.9240220 0.0004734615 8.450708e-01 9.036147e-01 2 99402889 99403039 151 - 1.589 1.600 0.037
ENSG00000135945 E015 18.3886062 0.0009646498 4.718735e-01 6.109545e-01 2 99403040 99403055 16 - 1.212 1.276 0.223
ENSG00000135945 E016 25.3844891 0.0008093309 5.420738e-01 6.729221e-01 2 99403056 99403106 51 - 1.352 1.398 0.160
ENSG00000135945 E017 2.8944295 0.0061610204 9.359021e-01 9.636812e-01 2 99403107 99403430 324 - 0.554 0.539 -0.067
ENSG00000135945 E018 39.5249958 0.0005843914 1.505884e-01 2.624818e-01 2 99403695 99403815 121 - 1.525 1.613 0.301
ENSG00000135945 E019 75.8756840 0.0003586060 6.776183e-01 7.822837e-01 2 99404444 99404677 234 - 1.823 1.841 0.061
ENSG00000135945 E020 34.0108244 0.0025551740 1.340900e-01 2.398383e-01 2 99405910 99405927 18 - 1.455 1.558 0.352
ENSG00000135945 E021 29.0694468 0.0007422824 1.432006e-01 2.523786e-01 2 99405928 99405932 5 - 1.390 1.494 0.357
ENSG00000135945 E022 57.2972128 0.0005367956 3.592046e-01 5.035424e-01 2 99405933 99406083 151 - 1.695 1.742 0.160
ENSG00000135945 E023 29.5206350 0.0016587385 4.642826e-01 6.040169e-01 2 99406084 99406106 23 - 1.414 1.467 0.184
ENSG00000135945 E024 0.6017953 0.1637331508 4.123660e-01 5.559300e-01 2 99406107 99406324 218 - 0.228 0.000 -11.058
ENSG00000135945 E025 46.4011719 0.0025322162 1.877525e-01 3.109028e-01 2 99406325 99406431 107 - 1.636 1.550 -0.295
ENSG00000135945 E026 30.8294414 0.0013169970 9.511493e-01 9.734446e-01 2 99406432 99406490 59 - 1.444 1.439 -0.018
ENSG00000135945 E027 3.6472332 0.0517721674 9.663362e-01 9.828654e-01 2 99406491 99406928 438 - 0.612 0.613 0.006
ENSG00000135945 E028 39.9652452 0.0005603882 3.221184e-02 7.625949e-02 2 99408029 99408131 103 - 1.585 1.439 -0.501
ENSG00000135945 E029 1.5855339 0.0091320969 2.003901e-01 3.267038e-01 2 99408132 99408403 272 - 0.308 0.539 1.255
ENSG00000135945 E030 50.3313322 0.0005462938 2.558768e-02 6.309396e-02 2 99410695 99410867 173 - 1.679 1.543 -0.465
ENSG00000135945 E031 38.6117853 0.0005664877 2.611328e-01 3.987589e-01 2 99412731 99412840 110 - 1.553 1.476 -0.262
ENSG00000135945 E032 38.6888066 0.0046760996 6.975384e-02 1.429616e-01 2 99412841 99412951 111 - 1.570 1.430 -0.480
ENSG00000135945 E033 0.1472490 0.0432519006 1.000000e+00   2 99412952 99412953 2 - 0.069 0.000 -9.270
ENSG00000135945 E034 23.9171016 0.0007981989 4.526934e-02 1.006849e-01 2 99418828 99418831 4 - 1.375 1.198 -0.618
ENSG00000135945 E035 44.4491232 0.0006307999 2.916389e-02 7.031514e-02 2 99418832 99418947 116 - 1.628 1.485 -0.487
ENSG00000135945 E036 52.6275914 0.0015673368 4.663793e-02 1.031879e-01 2 99421499 99421653 155 - 1.696 1.572 -0.420
ENSG00000135945 E037 42.4481217 0.0286177973 8.091722e-02 1.609659e-01 2 99424152 99424280 129 - 1.617 1.433 -0.631
ENSG00000135945 E038 2.8891164 0.0145831896 2.761019e-01 4.155083e-01 2 99424281 99424634 354 - 0.486 0.672 0.841
ENSG00000135945 E039 1.0404339 0.0119685750 6.212724e-01 7.382522e-01 2 99424781 99424791 11 - 0.308 0.208 -0.745
ENSG00000135945 E040 3.2685492 0.0286293328 7.834683e-01 8.610429e-01 2 99424792 99424899 108 - 0.594 0.540 -0.247
ENSG00000135945 E041 31.9582149 0.0045848283 3.420427e-02 8.015308e-02 2 99429840 99429945 106 - 1.495 1.317 -0.616
ENSG00000135945 E042 6.7025838 0.0145968683 1.752576e-03 6.448499e-03 2 99429946 99429948 3 - 0.924 0.349 -2.584
ENSG00000135945 E043 3.8953235 0.0068549566 4.764037e-01 6.150262e-01 2 99431741 99431800 60 - 0.664 0.540 -0.551
ENSG00000135945 E044 5.2489168 0.0257957393 8.880194e-01 9.324241e-01 2 99431801 99431940 140 - 0.752 0.725 -0.107
ENSG00000135945 E045 34.8206932 0.0005997637 3.676398e-03 1.222929e-02 2 99434332 99434448 117 - 1.536 1.316 -0.759
ENSG00000135945 E046 0.1472490 0.0432519006 1.000000e+00   2 99435475 99435833 359 - 0.069 0.000 -9.270
ENSG00000135945 E047 16.4036902 0.0110702201 4.292848e-02 9.640892e-02 2 99435834 99435836 3 - 1.226 0.985 -0.870
ENSG00000135945 E048 31.8700410 0.0006966715 5.819589e-05 3.225770e-04 2 99435837 99435941 105 - 1.516 1.181 -1.166
ENSG00000135945 E049 0.7490443 0.0779204142 2.712040e-01 4.099246e-01 2 99436647 99436791 145 - 0.270 0.000 -11.470
ENSG00000135945 E050 3.3654471 0.0045639968 5.506886e-01 6.802928e-01 2 99438574 99438600 27 - 0.574 0.671 0.425
ENSG00000135945 E051 61.6915129 0.0033114269 3.982964e-01 5.420265e-01 2 99438601 99438817 217 - 1.748 1.697 -0.175
ENSG00000135945 E052 112.0823965 0.0002784911 8.139019e-01 8.820507e-01 2 99438818 99439310 493 - 1.993 2.001 0.027
ENSG00000135945 E053 59.8343535 0.0031065955 2.493285e-01 3.851290e-01 2 99442317 99442469 153 - 1.742 1.673 -0.233
ENSG00000135945 E054 56.8453447 0.0007057384 9.806143e-01 9.918587e-01 2 99449336 99449504 169 - 1.704 1.701 -0.008
ENSG00000135945 E055 9.6295647 0.0110252699 8.921448e-01 9.351076e-01 2 99451345 99451501 157 - 0.975 0.955 -0.074
ENSG00000135945 E056 38.5201127 0.0007941283 4.071076e-01 5.507181e-01 2 99462496 99462622 127 - 1.521 1.572 0.177
ENSG00000135945 E057 0.2966881 0.0290785164 1.000000e+00   2 99462623 99462788 166 - 0.128 0.000 -10.250
ENSG00000135945 E058 25.8453185 0.0007419379 2.100344e-01 3.385782e-01 2 99464922 99464985 64 - 1.393 1.290 -0.361
ENSG00000135945 E059 26.1085389 0.0029544148 3.685785e-02 8.519637e-02 2 99489817 99490035 219 - 1.414 1.231 -0.640