ENSG00000135932

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000258418 ENSG00000135932 HEK293_OSMI2_2hA HEK293_TMG_2hB CAB39 protein_coding protein_coding 19.28147 8.343306 32.59775 0.2976194 1.261359 1.964795 8.338914 1.538704 19.20104 0.2585255 0.4000867 3.6328020 0.3538750 0.1844000 0.5918333 0.4074333 1.543249e-08 1.543249e-08 FALSE TRUE
MSTRG.20034.5 ENSG00000135932 HEK293_OSMI2_2hA HEK293_TMG_2hB CAB39 protein_coding   19.28147 8.343306 32.59775 0.2976194 1.261359 1.964795 10.060769 6.604370 11.68126 0.3591799 1.9466100 0.8217547 0.5971667 0.7918333 0.3547000 -0.4371333 7.732740e-07 1.543249e-08 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000135932 E001 31.4930281 0.0006465388 1.584916e-12 3.756976e-11 2 230712842 230713082 241 + 1.467 0.802 -2.407
ENSG00000135932 E002 66.1172792 0.0041090763 4.164505e-08 4.564116e-07 2 230713083 230713230 148 + 1.751 1.488 -0.895
ENSG00000135932 E003 1.1696869 0.1872268832 1.866741e-01 3.095629e-01 2 230713231 230713260 30 + 0.324 0.000 -10.573
ENSG00000135932 E004 1.6274528 0.0089143915 9.739119e-01 9.877336e-01 2 230713548 230713802 255 + 0.324 0.403 0.460
ENSG00000135932 E005 3.7268682 0.0053076286 5.248238e-01 6.582552e-01 2 230713803 230714058 256 + 0.544 0.746 0.873
ENSG00000135932 E006 0.8772652 0.0933620817 2.518802e-01 3.881780e-01 2 230720480 230720578 99 + 0.263 0.000 -10.359
ENSG00000135932 E007 100.6051506 0.0003336006 2.170475e-11 4.279662e-10 2 230759959 230760115 157 + 1.924 1.717 -0.699
ENSG00000135932 E008 0.0000000       2 230779006 230779450 445 +      
ENSG00000135932 E009 63.8870705 0.0003824601 7.536725e-05 4.060200e-04 2 230790872 230790897 26 + 1.718 1.609 -0.369
ENSG00000135932 E010 134.2186257 0.0002518637 2.713029e-08 3.081165e-07 2 230790898 230791036 139 + 2.033 1.933 -0.336
ENSG00000135932 E011 139.1855775 0.0002683108 4.886571e-06 3.487460e-05 2 230793213 230793331 119 + 2.042 1.991 -0.173
ENSG00000135932 E012 177.7138487 0.0002045283 2.531282e-10 4.163791e-09 2 230798729 230798897 169 + 2.155 2.059 -0.318
ENSG00000135932 E013 1.4197372 0.0965849791 8.362616e-01 8.976149e-01 2 230798898 230799124 227 + 0.295 0.403 0.649
ENSG00000135932 E014 122.5310010 0.0002495282 1.862548e-09 2.624427e-08 2 230810263 230810322 60 + 2.000 1.866 -0.449
ENSG00000135932 E015 0.1472490 0.0448877354 1.000000e+00   2 230813983 230814048 66 + 0.056 0.000 -7.961
ENSG00000135932 E016 105.0057681 0.0003163853 2.451692e-04 1.155186e-03 2 230814049 230814114 66 + 1.918 1.880 -0.129
ENSG00000135932 E017 202.4277894 0.0001958247 1.889912e-07 1.819669e-06 2 230817754 230817897 144 + 2.201 2.162 -0.132
ENSG00000135932 E018 2.3981678 0.0061113871 4.522253e-01 5.931001e-01 2 230817898 230818182 285 + 0.469 0.402 -0.349
ENSG00000135932 E019 1343.7407469 0.0026127112 3.260272e-34 7.578513e-32 2 230818516 230821075 2560 + 2.949 3.230 0.935