ENSG00000135930

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000258416 ENSG00000135930 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF4E2 protein_coding protein_coding 91.50038 118.5676 78.2484 16.87648 3.246765 -0.5995144 63.149704 83.275314 53.776045 13.522133 2.4129503 -0.6308302 0.68752500 0.69773333 0.68716667 -0.01056667 0.88540439 0.01376415 FALSE  
ENST00000409098 ENSG00000135930 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF4E2 protein_coding protein_coding 91.50038 118.5676 78.2484 16.87648 3.246765 -0.5995144 5.397554 3.503279 7.124214 1.308815 0.8555177 1.0219364 0.06216667 0.02736667 0.09086667 0.06350000 0.01376415 0.01376415 FALSE  
ENST00000409322 ENSG00000135930 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF4E2 protein_coding protein_coding 91.50038 118.5676 78.2484 16.87648 3.246765 -0.5995144 11.059734 17.607744 7.317910 1.432765 0.7825465 -1.2655554 0.11724583 0.15326667 0.09306667 -0.06020000 0.05641419 0.01376415 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000135930 E001 4.0502545 0.0151004346 5.654912e-01 6.925862e-01 2 232550601 232550658 58 + 0.633 0.728 0.399
ENSG00000135930 E002 6.5766642 0.0313503981 9.471196e-01 9.707854e-01 2 232550659 232550663 5 + 0.856 0.869 0.050
ENSG00000135930 E003 12.1690585 0.0149490075 8.591903e-01 9.131563e-01 2 232550664 232550673 10 + 1.084 1.100 0.058
ENSG00000135930 E004 14.7027533 0.0131823561 6.187921e-01 7.363448e-01 2 232550674 232550676 3 + 1.139 1.188 0.174
ENSG00000135930 E005 30.3702524 0.0086888397 2.280808e-01 3.601308e-01 2 232550677 232550682 6 + 1.411 1.503 0.318
ENSG00000135930 E006 44.3289297 0.0006125518 8.962754e-01 9.377095e-01 2 232550683 232550688 6 + 1.638 1.637 -0.003
ENSG00000135930 E007 47.4438846 0.0010450799 5.103027e-01 6.455350e-01 2 232550689 232550690 2 + 1.685 1.655 -0.102
ENSG00000135930 E008 47.4438846 0.0010450799 5.103027e-01 6.455350e-01 2 232550691 232550691 1 + 1.685 1.655 -0.102
ENSG00000135930 E009 101.4702835 0.0033714443 6.346394e-03 1.952428e-02 2 232550692 232550703 12 + 2.068 1.945 -0.411
ENSG00000135930 E010 384.9631600 0.0016091147 4.183716e-02 9.442717e-02 2 232550704 232550744 41 + 2.602 2.547 -0.185
ENSG00000135930 E011 0.5953508 0.0187134223 6.233062e-01 7.399225e-01 2 232550806 232550806 1 + 0.261 0.180 -0.685
ENSG00000135930 E012 0.5953508 0.0187134223 6.233062e-01 7.399225e-01 2 232550807 232550835 29 + 0.261 0.180 -0.685
ENSG00000135930 E013 8.3767980 0.0195361537 2.499493e-01 3.858519e-01 2 232550836 232551218 383 + 1.037 0.887 -0.560
ENSG00000135930 E014 0.1451727 0.0434144725 3.149916e-01   2 232553855 232553887 33 + 0.150 0.000 -9.323
ENSG00000135930 E015 666.4951048 0.0003404664 5.760434e-03 1.798298e-02 2 232556416 232556530 115 + 2.833 2.793 -0.133
ENSG00000135930 E016 2.5621772 0.1161471334 2.447694e-01 3.797682e-01 2 232556531 232557528 998 + 0.674 0.475 -0.906
ENSG00000135930 E017 1.0768638 0.0131335230 7.869529e-01 8.635800e-01 2 232557529 232557883 355 + 0.350 0.307 -0.270
ENSG00000135930 E018 687.7124871 0.0004467879 8.237185e-01 8.889287e-01 2 232557884 232558018 135 + 2.817 2.824 0.023
ENSG00000135930 E019 4.5275672 0.0035392202 2.702203e-02 6.604367e-02 2 232558019 232558821 803 + 0.903 0.611 -1.185
ENSG00000135930 E020 2.6850755 0.1425593139 3.148405e-02 7.485037e-02 2 232558822 232559813 992 + 0.802 0.353 -2.094
ENSG00000135930 E021 680.4643427 0.0001262496 4.336689e-02 9.723444e-02 2 232564247 232564351 105 + 2.796 2.831 0.117
ENSG00000135930 E022 682.2205102 0.0003870237 1.787025e-01 2.995192e-01 2 232566829 232566943 115 + 2.802 2.829 0.090
ENSG00000135930 E023 374.6962207 0.0021119028 3.278793e-01 4.713488e-01 2 232566944 232566981 38 + 2.536 2.569 0.111
ENSG00000135930 E024 244.2555175 0.0028714773 3.201435e-01 4.632582e-01 2 232567078 232567081 4 + 2.348 2.386 0.126
ENSG00000135930 E025 706.8221120 0.0004160231 1.659792e-08 1.964407e-07 2 232567082 232567214 133 + 2.769 2.866 0.320
ENSG00000135930 E026 179.7011016 0.0068357720 1.157012e-01 2.138171e-01 2 232567215 232568944 1730 + 2.188 2.260 0.241
ENSG00000135930 E027 671.6663804 0.0015088976 1.608505e-15 5.921618e-14 2 232568945 232569210 266 + 2.681 2.863 0.606
ENSG00000135930 E028 1.6251861 0.0085929300 8.962025e-01 9.376746e-01 2 232569741 232569833 93 + 0.423 0.405 -0.098
ENSG00000135930 E029 7.8741856 0.0044465461 6.172646e-04 2.596738e-03 2 232572016 232574073 2058 + 1.139 0.750 -1.465
ENSG00000135930 E030 0.9858280 0.0610418497 8.220037e-01 8.877736e-01 2 232574074 232574136 63 + 0.261 0.307 0.317
ENSG00000135930 E031 1.3953098 0.0101772518 8.678220e-02 1.702543e-01 2 232574137 232574262 126 + 0.541 0.248 -1.682
ENSG00000135930 E032 25.9093235 0.0010205349 1.750507e-01 2.947440e-01 2 232574263 232574341 79 + 1.469 1.382 -0.300
ENSG00000135930 E033 1002.5300995 0.0004591831 5.183455e-32 9.999899e-30 2 232580904 232583644 2741 + 3.082 2.923 -0.528