ENSG00000135924

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000336576 ENSG00000135924 HEK293_OSMI2_2hA HEK293_TMG_2hB DNAJB2 protein_coding protein_coding 21.68991 34.08367 13.65144 1.487324 0.8355036 -1.319395 4.6535988 8.464143 2.354373 0.5330567 0.2842647 -1.841610 0.1965625 0.2486667 0.17513333 -0.07353333 2.435574e-01 9.907728e-10 FALSE TRUE
ENST00000392086 ENSG00000135924 HEK293_OSMI2_2hA HEK293_TMG_2hB DNAJB2 protein_coding protein_coding 21.68991 34.08367 13.65144 1.487324 0.8355036 -1.319395 10.6304103 14.086047 6.279710 0.6512383 0.1644080 -1.164225 0.4886292 0.4153000 0.46270000 0.04740000 6.648911e-01 9.907728e-10 FALSE TRUE
ENST00000463463 ENSG00000135924 HEK293_OSMI2_2hA HEK293_TMG_2hB DNAJB2 protein_coding processed_transcript 21.68991 34.08367 13.65144 1.487324 0.8355036 -1.319395 0.4582613 0.000000 1.499655 0.0000000 0.8033642 7.238075 0.0331625 0.0000000 0.10433333 0.10433333 1.042698e-01 9.907728e-10   FALSE
ENST00000472019 ENSG00000135924 HEK293_OSMI2_2hA HEK293_TMG_2hB DNAJB2 protein_coding retained_intron 21.68991 34.08367 13.65144 1.487324 0.8355036 -1.319395 3.6041680 10.208228 1.076921 1.4275228 0.0386759 -3.232826 0.1500125 0.2974000 0.07913333 -0.21826667 9.907728e-10 9.907728e-10   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000135924 E001 1.3148701 0.0553283388 3.962244e-02 9.033024e-02 2 219279342 219279343 2 + 0.573 0.199 -2.232
ENSG00000135924 E002 2.0104414 0.1722207076 7.386541e-02 1.496896e-01 2 219279344 219279347 4 + 0.683 0.299 -1.949
ENSG00000135924 E003 3.2605760 0.0583967791 1.807740e-01 3.021677e-01 2 219279348 219279365 18 + 0.731 0.498 -1.026
ENSG00000135924 E004 3.8056891 0.1453139812 4.401090e-01 5.818132e-01 2 219279366 219279366 1 + 0.730 0.576 -0.659
ENSG00000135924 E005 3.9572045 0.0796375227 2.464159e-01 3.817569e-01 2 219279367 219279368 2 + 0.773 0.574 -0.842
ENSG00000135924 E006 21.1831057 0.0141882442 1.859177e-02 4.843648e-02 2 219279369 219279449 81 + 1.418 1.210 -0.729
ENSG00000135924 E007 22.3246112 0.0099572248 9.031596e-02 1.756196e-01 2 219279450 219279464 15 + 1.390 1.253 -0.478
ENSG00000135924 E008 37.5229872 0.0073062088 2.015598e-01 3.281308e-01 2 219279465 219279515 51 + 1.564 1.486 -0.268
ENSG00000135924 E009 29.1102438 0.0029524243 1.297520e-01 2.338143e-01 2 219279516 219279516 1 + 1.470 1.374 -0.331
ENSG00000135924 E010 28.6673534 0.0024347825 1.035882e-01 1.959587e-01 2 219279517 219279518 2 + 1.470 1.367 -0.357
ENSG00000135924 E011 2.5870530 0.0093248409 5.871837e-01 7.105276e-01 2 219279519 219279587 69 + 0.573 0.494 -0.370
ENSG00000135924 E012 3.6220746 0.0472805345 8.393281e-02 1.657008e-01 2 219279588 219279687 100 + 0.812 0.541 -1.146
ENSG00000135924 E013 4.2047399 0.0874231176 7.315487e-02 1.485657e-01 2 219279688 219279797 110 + 0.879 0.582 -1.222
ENSG00000135924 E014 77.6157554 0.0037729571 1.465498e-02 3.971632e-02 2 219279798 219279898 101 + 1.897 1.787 -0.369
ENSG00000135924 E015 1.2439081 0.1872480083 8.500630e-02 1.674119e-01 2 219279899 219279905 7 + 0.572 0.198 -2.245
ENSG00000135924 E016 1.2439081 0.1872480083 8.500630e-02 1.674119e-01 2 219279906 219279919 14 + 0.572 0.198 -2.245
ENSG00000135924 E017 2.0889185 0.1985260259 6.606787e-02 1.368267e-01 2 219279920 219279975 56 + 0.730 0.295 -2.167
ENSG00000135924 E018 1.3234029 0.0805706323 4.548277e-02 1.010816e-01 2 219279976 219280059 84 + 0.573 0.200 -2.230
ENSG00000135924 E019 0.6537541 0.5083673911 4.480916e-01 5.894130e-01 2 219280060 219280170 111 + 0.320 0.143 -1.484
ENSG00000135924 E020 73.5132685 0.0008694737 2.075818e-03 7.459217e-03 2 219280578 219280612 35 + 1.884 1.760 -0.421
ENSG00000135924 E021 99.3615805 0.0003709742 3.337133e-03 1.125449e-02 2 219280613 219280687 75 + 1.996 1.899 -0.326
ENSG00000135924 E022 3.7559112 0.0043790735 2.651145e-05 1.597594e-04 2 219280688 219280839 152 + 0.990 0.406 -2.504
ENSG00000135924 E023 15.5024834 0.0042524839 2.794790e-11 5.410982e-10 2 219280840 219281717 878 + 1.501 0.922 -2.059
ENSG00000135924 E024 102.1701384 0.0002924855 2.526910e-02 6.242623e-02 2 219281718 219281771 54 + 1.989 1.921 -0.229
ENSG00000135924 E025 78.9834646 0.0031826215 2.463418e-01 3.816607e-01 2 219281939 219281959 21 + 1.859 1.820 -0.130
ENSG00000135924 E026 140.3145873 0.0003323039 2.852449e-02 6.906534e-02 2 219281960 219282061 102 + 2.115 2.061 -0.182
ENSG00000135924 E027 2.9474325 0.0053068420 5.935611e-04 2.510120e-03 2 219282062 219282478 417 + 0.879 0.371 -2.283
ENSG00000135924 E028 2.8127552 0.0317024446 2.973757e-03 1.018493e-02 2 219282479 219282697 219 + 0.847 0.371 -2.163
ENSG00000135924 E029 1.2941444 0.0102320805 2.814996e-02 6.830954e-02 2 219282698 219282741 44 + 0.573 0.199 -2.241
ENSG00000135924 E030 2.6290737 0.0058044843 9.294198e-03 2.701967e-02 2 219282742 219282836 95 + 0.773 0.372 -1.866
ENSG00000135924 E031 171.0356479 0.0002230467 4.315764e-02 9.683289e-02 2 219282837 219282929 93 + 2.189 2.147 -0.141
ENSG00000135924 E032 3.6254858 0.0060673319 9.823878e-04 3.899642e-03 2 219282930 219283063 134 + 0.910 0.438 -2.034
ENSG00000135924 E033 4.3522135 0.0038376255 2.918197e-01 4.327284e-01 2 219283064 219283132 69 + 0.773 0.632 -0.587
ENSG00000135924 E034 189.4221149 0.0002511000 4.370052e-01 5.790391e-01 2 219283133 219283235 103 + 2.204 2.201 -0.011
ENSG00000135924 E035 3.0163156 0.0052096566 8.114133e-02 1.613262e-01 2 219283236 219283418 183 + 0.731 0.467 -1.181
ENSG00000135924 E036 180.3558532 0.0028645074 9.208205e-01 9.540634e-01 2 219283419 219283489 71 + 2.160 2.184 0.078
ENSG00000135924 E037 247.1803470 0.0001947769 6.081224e-02 1.280049e-01 2 219284632 219284835 204 + 2.262 2.331 0.230
ENSG00000135924 E038 93.0209617 0.0003134739 4.790376e-03 1.534792e-02 2 219284836 219285262 427 + 1.776 1.922 0.493
ENSG00000135924 E039 130.9454570 0.0044908306 6.572557e-02 1.362474e-01 2 219285263 219285776 514 + 1.956 2.064 0.363
ENSG00000135924 E040 35.1001831 0.0005971450 1.552641e-03 5.806679e-03 2 219285777 219285800 24 + 1.268 1.527 0.897
ENSG00000135924 E041 76.0002311 0.0003706190 6.956243e-03 2.110941e-02 2 219285801 219285986 186 + 1.682 1.837 0.521
ENSG00000135924 E042 274.0304302 0.0031520716 1.325680e-04 6.701698e-04 2 219285987 219286265 279 + 2.233 2.394 0.538
ENSG00000135924 E043 499.1202602 0.0052238133 5.544578e-04 2.363698e-03 2 219286266 219286898 633 + 2.505 2.650 0.481