ENSG00000135870

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000258349 ENSG00000135870 HEK293_OSMI2_2hA HEK293_TMG_2hB RC3H1 protein_coding protein_coding 2.349791 1.23178 3.473428 0.3363313 0.1541397 1.488098 1.4951444 0.94362091 2.0237802 0.27701594 0.4564248 1.092676 0.6490167 0.76060000 0.57410000 -0.186500000 0.44180722 0.04997653 FALSE TRUE
ENST00000367694 ENSG00000135870 HEK293_OSMI2_2hA HEK293_TMG_2hB RC3H1 protein_coding protein_coding 2.349791 1.23178 3.473428 0.3363313 0.1541397 1.488098 0.2428735 0.02733421 0.4791262 0.01390938 0.1086240 3.711637 0.0739125 0.02156667 0.14050000 0.118933333 0.04997653 0.04997653 FALSE TRUE
ENST00000367696 ENSG00000135870 HEK293_OSMI2_2hA HEK293_TMG_2hB RC3H1 protein_coding protein_coding 2.349791 1.23178 3.473428 0.3363313 0.1541397 1.488098 0.3847914 0.24581833 0.6281118 0.08542714 0.4781703 1.318690 0.1897542 0.20106667 0.19223333 -0.008833333 0.78750378 0.04997653 FALSE TRUE
MSTRG.2608.5 ENSG00000135870 HEK293_OSMI2_2hA HEK293_TMG_2hB RC3H1 protein_coding   2.349791 1.23178 3.473428 0.3363313 0.1541397 1.488098 0.1905490 0.01500664 0.2544795 0.01500664 0.1501874 3.402773 0.0621625 0.01676667 0.06976667 0.053000000 0.61626919 0.04997653 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000135870 E001 1.3284849 0.011106020 9.969123e-03 2.868762e-02 1 173931084 173931213 130 - 0.118 0.705 3.704
ENSG00000135870 E002 412.3784349 0.035587790 1.839483e-06 1.442284e-05 1 173931214 173938410 7197 - 2.384 2.846 1.538
ENSG00000135870 E003 11.5604877 0.002576365 5.513614e-02 1.182326e-01 1 173938411 173938575 165 - 1.015 1.048 0.121
ENSG00000135870 E004 7.0823997 0.046722703 1.121162e-01 2.086432e-01 1 173938576 173938592 17 - 0.850 0.805 -0.176
ENSG00000135870 E005 24.0695286 0.002799261 1.356930e-01 2.420818e-01 1 173938593 173938871 279 - 1.288 1.427 0.484
ENSG00000135870 E006 5.6289376 0.002984562 2.544637e-01 3.911531e-01 1 173938872 173938874 3 - 0.742 0.808 0.262
ENSG00000135870 E007 21.2108568 0.020212392 1.749719e-01 2.946297e-01 1 173941265 173941380 116 - 1.245 1.342 0.339
ENSG00000135870 E008 0.1515154 0.045827063 1.000000e+00   1 173941381 173941449 69 - 0.063 0.000 -16.097
ENSG00000135870 E009 22.2157986 0.000929722 2.555572e-01 3.924584e-01 1 173943442 173943588 147 - 1.248 1.417 0.588
ENSG00000135870 E010 8.7466393 0.002080849 2.705518e-02 6.610634e-02 1 173943589 173943615 27 - 0.929 0.891 -0.143
ENSG00000135870 E011 15.7531818 0.001178169 5.805509e-04 2.462026e-03 1 173946476 173946608 133 - 1.168 1.074 -0.339
ENSG00000135870 E012 8.0262888 0.002247048 3.068757e-02 7.330771e-02 1 173946746 173946836 91 - 0.896 0.852 -0.170
ENSG00000135870 E013 15.5654229 0.091183075 3.751561e-02 8.643309e-02 1 173947369 173947582 214 - 1.168 1.004 -0.594
ENSG00000135870 E014 13.9225549 0.001357648 1.830223e-04 8.907876e-04 1 173951986 173952138 153 - 1.125 0.961 -0.598
ENSG00000135870 E015 0.0000000       1 173961033 173961076 44 -      
ENSG00000135870 E016 16.5348072 0.097576609 3.824381e-02 8.780967e-02 1 173961077 173961244 168 - 1.195 1.028 -0.600
ENSG00000135870 E017 25.7532577 0.027621888 1.445234e-04 7.232545e-04 1 173961725 173962095 371 - 1.388 1.163 -0.787
ENSG00000135870 E018 19.3441036 0.001435731 3.033738e-05 1.803196e-04 1 173963973 173964187 215 - 1.259 1.121 -0.491
ENSG00000135870 E019 15.4753936 0.001515678 2.631188e-03 9.163334e-03 1 173964839 173965120 282 - 1.149 1.098 -0.184
ENSG00000135870 E020 8.9501666 0.002649908 5.134513e-05 2.885119e-04 1 173970505 173970617 113 - 0.981 0.643 -1.339
ENSG00000135870 E021 9.3725839 0.021058597 1.486029e-02 4.017372e-02 1 173972509 173972627 119 - 0.967 0.851 -0.440
ENSG00000135870 E022 13.5172497 0.002600878 2.663786e-02 6.524023e-02 1 173978488 173978620 133 - 1.082 1.098 0.058
ENSG00000135870 E023 14.3966904 0.008694246 4.045501e-02 9.186021e-02 1 173980809 173980948 140 - 1.104 1.122 0.064
ENSG00000135870 E024 10.0621647 0.001982594 6.982202e-03 2.117699e-02 1 173980949 173981009 61 - 0.982 0.891 -0.338
ENSG00000135870 E025 14.4058306 0.004781342 4.140441e-04 1.828297e-03 1 173982727 173982902 176 - 1.140 0.992 -0.537
ENSG00000135870 E026 0.0000000       1 173982903 173983033 131 -      
ENSG00000135870 E027 17.2481511 0.001278274 2.761830e-06 2.084295e-05 1 173983418 173983657 240 - 1.224 0.992 -0.837
ENSG00000135870 E028 11.1130079 0.004790975 1.736757e-03 6.398371e-03 1 173984499 173984619 121 - 1.035 0.891 -0.535
ENSG00000135870 E029 14.9870113 0.001380334 1.959551e-03 7.099196e-03 1 173992755 173993135 381 - 1.140 1.074 -0.237
ENSG00000135870 E030 6.0966464 0.087524967 9.044239e-02 1.758236e-01 1 174022096 174022358 263 - 0.809 0.646 -0.669