ENSG00000135686

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000325279 ENSG00000135686 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL36 protein_coding retained_intron 9.723332 11.28252 7.781092 1.07941 0.3525091 -0.5354706 1.2289104 1.3059140 0.8467810 0.1606013 0.16887299 -0.6190669 0.12862083 0.11836667 0.1078333 -0.01053333 0.91949753 0.02385849 FALSE TRUE
ENST00000564159 ENSG00000135686 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL36 protein_coding retained_intron 9.723332 11.28252 7.781092 1.07941 0.3525091 -0.5354706 1.1100023 0.9359826 1.0909921 0.1701127 0.07936614 0.2189186 0.11797500 0.08263333 0.1403000 0.05766667 0.08478133 0.02385849 FALSE TRUE
ENST00000564996 ENSG00000135686 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL36 protein_coding protein_coding 9.723332 11.28252 7.781092 1.07941 0.3525091 -0.5354706 3.8374486 4.3186964 3.7650505 0.2327861 0.14765415 -0.1974366 0.40377500 0.38910000 0.4865667 0.09746667 0.36640970 0.02385849 FALSE TRUE
ENST00000567410 ENSG00000135686 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL36 protein_coding protein_coding 9.723332 11.28252 7.781092 1.07941 0.3525091 -0.5354706 0.6358851 0.3834533 0.8535623 0.1393193 0.20734139 1.1341078 0.06499167 0.03246667 0.1089667 0.07650000 0.03743420 0.02385849 FALSE TRUE
MSTRG.13154.3 ENSG00000135686 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL36 protein_coding   9.723332 11.28252 7.781092 1.07941 0.3525091 -0.5354706 2.6987059 4.0431895 1.1034493 0.7392838 0.29267249 -1.8640217 0.26195417 0.35190000 0.1416000 -0.21030000 0.02385849 0.02385849 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000135686 E001 1.6210214 0.0084963996 2.038042e-01 3.309711e-01 16 84648511 84648518 8 + 0.533 0.313 -1.195
ENSG00000135686 E002 5.3488568 0.0625648342 1.034913e-01 1.958144e-01 16 84648519 84648534 16 + 0.937 0.672 -1.048
ENSG00000135686 E003 14.5695893 0.0387382128 4.375199e-01 5.794864e-01 16 84648535 84648611 77 + 1.223 1.128 -0.339
ENSG00000135686 E004 12.3070292 0.0350207445 5.893486e-01 7.124192e-01 16 84648612 84648649 38 + 1.044 1.118 0.270
ENSG00000135686 E005 5.3583850 0.0030256086 3.427178e-04 1.551011e-03 16 84648650 84648887 238 + 1.027 0.560 -1.873
ENSG00000135686 E006 0.1817044 0.0391195507 7.039128e-01   16 84648922 84648965 44 + 0.000 0.102 9.210
ENSG00000135686 E007 0.4751703 0.0220427107 8.291670e-01 8.927058e-01 16 84648966 84649066 101 + 0.147 0.184 0.388
ENSG00000135686 E008 1.9747766 0.1910470474 4.699121e-01 6.091320e-01 16 84649067 84649318 252 + 0.343 0.524 0.957
ENSG00000135686 E009 28.4323978 0.0051179793 4.297667e-01 5.724367e-01 16 84650852 84650930 79 + 1.407 1.463 0.193
ENSG00000135686 E010 257.7032368 0.0038947899 6.103231e-02 1.283711e-01 16 84656871 84657902 1032 + 2.349 2.412 0.209
ENSG00000135686 E011 31.8471565 0.0067759638 3.697101e-01 5.141075e-01 16 84657903 84657913 11 + 1.452 1.517 0.222
ENSG00000135686 E012 42.4419461 0.0005794330 4.250317e-01 5.679771e-01 16 84657914 84657944 31 + 1.589 1.630 0.139
ENSG00000135686 E013 56.5599777 0.0113335027 8.578020e-03 2.523416e-02 16 84657945 84659538 1594 + 1.841 1.663 -0.602
ENSG00000135686 E014 11.1027642 0.0071351612 7.161034e-01 8.118388e-01 16 84659539 84659630 92 + 1.027 1.064 0.133
ENSG00000135686 E015 11.6789599 0.0104184793 7.978406e-01 8.710022e-01 16 84659631 84659759 129 + 1.059 1.083 0.089
ENSG00000135686 E016 100.4182782 0.0014253997 1.717444e-01 2.904782e-01 16 84659760 84659917 158 + 1.948 1.999 0.172
ENSG00000135686 E017 199.9693158 0.0002091274 2.216482e-06 1.707004e-05 16 84661578 84662104 527 + 2.201 2.319 0.392
ENSG00000135686 E018 132.7750435 0.0002745838 1.717905e-06 1.355485e-05 16 84662105 84663027 923 + 2.194 2.046 -0.495
ENSG00000135686 E019 30.0721472 0.0006748974 3.654404e-03 1.216788e-02 16 84663028 84663165 138 + 1.580 1.393 -0.641
ENSG00000135686 E020 662.0292641 0.0042968227 4.881592e-01 6.257675e-01 16 84663166 84667686 4521 + 2.812 2.796 -0.056
ENSG00000135686 E021 0.1817044 0.0391195507 7.039128e-01   16 84674010 84674066 57 + 0.000 0.102 9.210