ENSG00000135677

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000258145 ENSG00000135677 HEK293_OSMI2_2hA HEK293_TMG_2hB GNS protein_coding protein_coding 26.80159 20.33275 29.33471 1.919829 1.115769 0.5285861 24.5453938 18.295342 27.69658037 2.030036 0.36354844 0.597964 0.91743333 0.90253333 0.946033333 0.0435 0.850278347 0.006679992 FALSE  
ENST00000418919 ENSG00000135677 HEK293_OSMI2_2hA HEK293_TMG_2hB GNS protein_coding protein_coding 26.80159 20.33275 29.33471 1.919829 1.115769 0.5285861 0.8561053 1.964707 0.07989851 1.325259 0.03998879 -4.457197 0.03870833 0.09393333 0.002633333 -0.0913 0.006679992 0.006679992 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000135677 E001 1.7713012 0.0148598042 9.538805e-03 2.763358e-02 12 64713445 64713448 4 - 0.179 0.674 2.866
ENSG00000135677 E002 2111.7820575 0.0040345792 1.887641e-14 5.967093e-13 12 64713449 64716461 3013 - 3.210 3.435 0.746
ENSG00000135677 E003 113.5833680 0.0022424444 1.475784e-02 3.994554e-02 12 64716462 64716474 13 - 2.063 2.045 -0.061
ENSG00000135677 E004 191.4557730 0.0004799114 1.830891e-01 3.050712e-01 12 64716475 64716608 134 - 2.262 2.307 0.150
ENSG00000135677 E005 242.4756107 0.0001889642 4.668252e-01 6.063638e-01 12 64716609 64716819 211 - 2.355 2.419 0.214
ENSG00000135677 E006 99.3754213 0.0002827537 9.715336e-01 9.862033e-01 12 64720022 64720038 17 - 1.963 2.045 0.275
ENSG00000135677 E007 100.6518712 0.0003209115 9.080661e-01 9.456428e-01 12 64720039 64720050 12 - 1.970 2.047 0.256
ENSG00000135677 E008 192.2335418 0.0002368901 2.710915e-01 4.097845e-01 12 64720051 64720182 132 - 2.261 2.314 0.175
ENSG00000135677 E009 188.8207448 0.0012193632 1.446815e-01 2.544015e-01 12 64721595 64721705 111 - 2.259 2.295 0.118
ENSG00000135677 E010 0.3030308 0.3706965756 4.046538e-01   12 64722833 64723005 173 - 0.180 0.000 -9.810
ENSG00000135677 E011 197.4187402 0.0002356168 3.625280e-06 2.664798e-05 12 64723006 64723113 108 - 2.311 2.272 -0.129
ENSG00000135677 E012 194.2107042 0.0002412544 1.712402e-06 1.351988e-05 12 64728956 64729057 102 - 2.306 2.262 -0.147
ENSG00000135677 E013 0.7468662 0.0589749969 8.968040e-01 9.380659e-01 12 64729058 64729218 161 - 0.247 0.261 0.105
ENSG00000135677 E014 178.8634084 0.0008244226 1.118412e-06 9.191785e-06 12 64737004 64737107 104 - 2.279 2.215 -0.213
ENSG00000135677 E015 112.3630809 0.0037568712 1.375484e-03 5.226740e-03 12 64739381 64739403 23 - 2.079 2.016 -0.213
ENSG00000135677 E016 167.1000044 0.0002500411 2.950109e-07 2.734426e-06 12 64739404 64739499 96 - 2.250 2.186 -0.211
ENSG00000135677 E017 163.2274835 0.0033555358 2.832086e-05 1.694449e-04 12 64740606 64740688 83 - 2.247 2.160 -0.289
ENSG00000135677 E018 198.5987415 0.0024186506 2.330700e-05 1.423096e-04 12 64743141 64743308 168 - 2.324 2.257 -0.221
ENSG00000135677 E019 118.6606976 0.0034513453 2.368430e-02 5.916062e-02 12 64744809 64744907 99 - 2.082 2.063 -0.064
ENSG00000135677 E020 94.1698782 0.0003350978 3.764351e-04 1.683097e-03 12 64745659 64745724 66 - 1.997 1.945 -0.174
ENSG00000135677 E021 0.8084787 0.1344851478 3.614024e-01 5.057850e-01 12 64745725 64746238 514 - 0.305 0.152 -1.284
ENSG00000135677 E022 176.5977956 0.0008268818 6.809959e-14 1.981152e-12 12 64747712 64747918 207 - 2.302 2.156 -0.489
ENSG00000135677 E023 97.0893432 0.0107282787 1.818204e-04 8.857799e-04 12 64752698 64752757 60 - 2.046 1.892 -0.515
ENSG00000135677 E024 5.6985123 0.0041727109 2.663116e-01 4.044859e-01 12 64752758 64752844 87 - 0.853 0.777 -0.298
ENSG00000135677 E025 0.0000000       12 64752845 64752971 127 -      
ENSG00000135677 E026 0.2214452 0.0563815699 3.632735e-01   12 64756667 64756762 96 - 0.000 0.151 9.993
ENSG00000135677 E027 148.4969138 0.0070884787 3.880076e-05 2.243389e-04 12 64759085 64759431 347 - 2.220 2.093 -0.425